Vladimir Radosavljevic, Dimitrije Glisic, Jelena Maksimovic-Zoric, Ljubisa Veljovic, Bozidar Savic
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引用次数: 0
摘要
2022 年 4 月,一家鲤鱼养殖场的 1 龄鲤鱼大量死亡。除死亡率高外,病鱼的皮肤和鳍上出现乳头状增生,这是鲤鱼痘的特征。为了调查可能的病毒原因,我们采集了组织样本,并使用标准程序提取核酸。通过实时聚合酶链式反应(real-time PCR),在鳃和肾的集合样本中检测到了鲤鱼水肿病毒(CEV)。在有乳头状增生的皮肤组织样本中,通过针对鲤科疱疹病毒 DNA 聚合酶保守区的传统 PCR 方法检测到了鲤科疱疹病毒 1(CyHV1)。PCR 产物通过琼脂糖凝胶电泳显现,CyHV1 DNA 的存在通过桑格测序得到证实。这是塞尔维亚首次从分子角度证实鲤鱼感染了 CyHV1。
First molecular confirmation of cyprinid herpesvirus 1 (CyHV1) in diseased carp in Serbia.
Mass mortality of farmed 1 yr old common carp Cyprinus carpio occurred at a carp farm in April 2022. In addition to high mortality, diseased fish exhibited papillomatous growths on the skin and fins, characteristic of carp pox. To investigate a possible viral cause, tissue samples were collected and nucleic acid was extracted using standard procedures. In a pooled sample from the gills and kidneys, carp edema virus (CEV) was detected by real-time PCR. In a skin tissue sample with papillomatous growths, cyprinid herpesvirus 1 (CyHV1) was detected by a conventional PCR targeting a conserved region of the DNA polymerase of cyprinid herpesviruses. PCR products were visualized through agarose gel electrophoresis, and the presence of CyHV1 DNA was confirmed by Sanger sequencing. This represents the first molecular confirmation of CyHV1 in common carp in Serbia.
期刊介绍:
DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically:
-Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens
-Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)-
Diseases due to internal circumstances (innate, idiopathic, genetic)-
Diseases due to proliferative disorders (neoplasms)-
Disease diagnosis, treatment and prevention-
Molecular aspects of diseases-
Nutritional disorders-
Stress and physical injuries-
Epidemiology/epizootiology-
Parasitology-
Toxicology-
Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)-
Diseases as indicators of humanity''s detrimental impact on nature-
Genomics, proteomics and metabolomics of disease-
Immunology and disease prevention-
Animal welfare-
Zoonosis