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引用次数: 0
摘要
摘要ABSTRACTCoxsackievirus B4(CVB4)与手足口病、心肌炎、脑膜炎、胰腺炎和 1 型糖尿病等一系列急性和慢性疾病有关,每年影响着全球数百万幼儿。然而,目前还没有预防 CVB4 感染的疫苗。在此,我们报告了 CVB4 病毒颗粒灭活疫苗的研发情况。我们从CVB4感染的细胞培养物中制备了两种灭活的CVB4颗粒作为疫苗抗原,包括由VP1、VP3、VP2和VP4亚基蛋白组成的F颗粒(又称成熟病毒体)和由VP1、VP3和未分化VP0组成的E颗粒(又称空壳)。灭活的 CVB4 F 颗粒和 E 颗粒都能有效激发小鼠体内的中和抗体,尽管在第三次免疫后,E 颗粒疫苗的中和抗体滴度略低。重要的是,我们证明了被动转移抗 F 粒子或抗 E 粒子血清可完全保护受体小鼠免受致命的 CVB4 挑战。我们的研究不仅确定了 CVB4 F 颗粒和 E 颗粒灭活疫苗的免疫原性和保护效力,还揭示了中和抗体在抗 CVB4 保护性免疫中的核心作用,从而为加速 CVB4 灭活疫苗的开发提供了重要信息。
Immunogenicity and protective efficacy of inactivated coxsackievirus B4 viral particles.
Coxsackievirus B4 (CVB4) is associated with a range of acute and chronic diseases such as hand, foot, and mouth disease, myocarditis, meningitis, pancreatitis, and type 1 diabetes, affecting millions of young children annually around the world. However, no vaccine is currently available for preventing CVB4 infection. Here, we report the development of inactivated viral particle vaccines for CVB4. Two types of inactivated CVB4 particles were prepared from CVB4-infected cell cultures as vaccine antigens, including F-particle (also called mature virion) consisting of VP1, VP3, VP2, and VP4 subunit proteins, and E-particle (also called empty capsid) which is made of VP1, VP3, and uncleaved VP0. Both the inactivated CVB4 F-particle and E-particle were able to potently elicit neutralizing antibodies in mice, despite slightly lower neutralizing antibody titres seen with the E-particle vaccine after the third immunization. Importantly, we demonstrated that passive transfer of either anti-F-particle or anti-E-particle sera could completely protect the recipient mice from lethal CVB4 challenge. Our study not only defines the immunogenicity and protective efficacy of inactivated CVB4 F-particle and E-particle but also reveals the central role of neutralizing antibodies in anti-CVB4 protective immunity, thus providing important information that may accelerate the development of inactivated CVB4 vaccines.
期刊介绍:
Emerging Microbes & Infections is a peer-reviewed, open-access journal dedicated to publishing research at the intersection of emerging immunology and microbiology viruses.
The journal's mission is to share information on microbes and infections, particularly those gaining significance in both biological and clinical realms due to increased pathogenic frequency. Emerging Microbes & Infections is committed to bridging the scientific gap between developed and developing countries.
This journal addresses topics of critical biological and clinical importance, including but not limited to:
- Epidemic surveillance
- Clinical manifestations
- Diagnosis and management
- Cellular and molecular pathogenesis
- Innate and acquired immune responses between emerging microbes and their hosts
- Drug discovery
- Vaccine development research
Emerging Microbes & Infections invites submissions of original research articles, review articles, letters, and commentaries, fostering a platform for the dissemination of impactful research in the field.