{"title":"使用 DNA 完整性编号 (DIN) 分析乳腺肿瘤和病变细针穿刺后液基细胞学样本中的 DNA 质量。","authors":"Akiyoshi Hoshino, Yoshiyasu Oana, Yasuyo Ohi, Yukari Maeda, Masako Omori, Yuki Takada, Tadashi Ikeda, Keiichi Sotome, Hinako Maeda, Takako Yanagisawa, Osamu Takeuchi, Satoshi Kuronuma, Takafumi Sangai, Yukiko Shibahara, Yoshiki Murakumo, Makoto Saegusa, Naoki Kanomata, Satoi Nagasawa, Rin Yamaguchi, Masayuki Yoshida, Yuji Kozuka, Hiroshi Matsumoto, Koichiro Tsugawa, Ichiro Maeda","doi":"10.1159/000538071","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Cancer genome analysis using next-generation sequencing requires adequate and high-quality DNA samples. Genomic analyses were conventionally performed using formalin-fixed paraffin-embedded sections rather than cytology samples such as cell block or smear specimens. Specimens collected from liquid-based cytology (LBC) have the potential to be sources of high-quality DNA suitable for genetic analysis even after long-term storage.</p><p><strong>Methods: </strong>We collected breast tumor/lesion fractions from 92 residual LBC specimens using fine-needle aspiration (FNA) biopsy, including breast carcinoma (1 invasive carcinoma and 4 ductal carcinomas in situ), papillomatous lesion (5 intraductal papillomas), and fibroepithelial lesion (19 phyllodes tumors and 53 fibroadenomas) samples, and others (1 ductal adenoma, 1 hamartoma, 1 fibrocystic disease, and 7 unknown). DNA was extracted from all samples and subjected to DNA integrity number (DIN) score analysis.</p><p><strong>Results: </strong>Average DIN score collected from 92 LBC specimens was significantly higher score. In addition, high-quality DNA with high DIN values (7.39 ± 0.80) was successfully extracted more than 12 months after storage of residual LBC specimens.</p><p><strong>Conclusion: </strong>Residual LBC specimens collected from FNA of the breast were verified to carry high-quality DNA and could serve as an alternate source for genetic analysis.</p>","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Using the DNA Integrity Number to Analyze DNA Quality in Specimens Collected from Liquid-Based Cytology after Fine-Needle Aspiration of Breast Tumors and Lesions.\",\"authors\":\"Akiyoshi Hoshino, Yoshiyasu Oana, Yasuyo Ohi, Yukari Maeda, Masako Omori, Yuki Takada, Tadashi Ikeda, Keiichi Sotome, Hinako Maeda, Takako Yanagisawa, Osamu Takeuchi, Satoshi Kuronuma, Takafumi Sangai, Yukiko Shibahara, Yoshiki Murakumo, Makoto Saegusa, Naoki Kanomata, Satoi Nagasawa, Rin Yamaguchi, Masayuki Yoshida, Yuji Kozuka, Hiroshi Matsumoto, Koichiro Tsugawa, Ichiro Maeda\",\"doi\":\"10.1159/000538071\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Cancer genome analysis using next-generation sequencing requires adequate and high-quality DNA samples. Genomic analyses were conventionally performed using formalin-fixed paraffin-embedded sections rather than cytology samples such as cell block or smear specimens. Specimens collected from liquid-based cytology (LBC) have the potential to be sources of high-quality DNA suitable for genetic analysis even after long-term storage.</p><p><strong>Methods: </strong>We collected breast tumor/lesion fractions from 92 residual LBC specimens using fine-needle aspiration (FNA) biopsy, including breast carcinoma (1 invasive carcinoma and 4 ductal carcinomas in situ), papillomatous lesion (5 intraductal papillomas), and fibroepithelial lesion (19 phyllodes tumors and 53 fibroadenomas) samples, and others (1 ductal adenoma, 1 hamartoma, 1 fibrocystic disease, and 7 unknown). DNA was extracted from all samples and subjected to DNA integrity number (DIN) score analysis.</p><p><strong>Results: </strong>Average DIN score collected from 92 LBC specimens was significantly higher score. In addition, high-quality DNA with high DIN values (7.39 ± 0.80) was successfully extracted more than 12 months after storage of residual LBC specimens.</p><p><strong>Conclusion: </strong>Residual LBC specimens collected from FNA of the breast were verified to carry high-quality DNA and could serve as an alternate source for genetic analysis.</p>\",\"PeriodicalId\":6959,\"journal\":{\"name\":\"Acta Cytologica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Cytologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1159/000538071\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/3/29 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"PATHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Cytologica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000538071","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/29 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"PATHOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景：使用新一代测序技术进行癌症基因组分析需要足够的高质量 DNA 样本。基因组分析通常使用福尔马林固定石蜡包埋（FFPE）切片，而不是细胞学样本，如细胞块或涂片标本。从液基细胞学（LBC）中收集的标本有可能成为高质量 DNA 的来源，即使经过长期储存也适合进行基因分析：我们使用细针穿刺活检术（FNA）从 92 个残留的 LBC 标本中收集了乳腺肿瘤/病变部分，包括乳腺癌（1 个浸润癌和 4 个导管原位癌）、乳头状瘤病变（5 个导管内乳头状瘤）和纤维上皮病变（19 个植物瘤和 53 个纤维腺瘤）样本，以及其他样本（1 个导管腺瘤、1 个火腿肠瘤、1 个纤维囊性疾病和 7 个未知样本）。从所有样本中提取 DNA 并进行 DNA 完整性编号（DIN）评分分析：结果：从 92 份 LBC 标本中收集到的平均 DIN 分数明显更高。此外，残留的 LBC 标本在保存 12 个月后，成功提取出了高质量的 DNA，其 DIN 值很高（7.39 ± 0.80）：结论：从乳腺 FNA 中采集的残留 LBC 标本经证实携带高质量 DNA，可作为基因分析的替代来源。
Using the DNA Integrity Number to Analyze DNA Quality in Specimens Collected from Liquid-Based Cytology after Fine-Needle Aspiration of Breast Tumors and Lesions.
Introduction: Cancer genome analysis using next-generation sequencing requires adequate and high-quality DNA samples. Genomic analyses were conventionally performed using formalin-fixed paraffin-embedded sections rather than cytology samples such as cell block or smear specimens. Specimens collected from liquid-based cytology (LBC) have the potential to be sources of high-quality DNA suitable for genetic analysis even after long-term storage.
Methods: We collected breast tumor/lesion fractions from 92 residual LBC specimens using fine-needle aspiration (FNA) biopsy, including breast carcinoma (1 invasive carcinoma and 4 ductal carcinomas in situ), papillomatous lesion (5 intraductal papillomas), and fibroepithelial lesion (19 phyllodes tumors and 53 fibroadenomas) samples, and others (1 ductal adenoma, 1 hamartoma, 1 fibrocystic disease, and 7 unknown). DNA was extracted from all samples and subjected to DNA integrity number (DIN) score analysis.
Results: Average DIN score collected from 92 LBC specimens was significantly higher score. In addition, high-quality DNA with high DIN values (7.39 ± 0.80) was successfully extracted more than 12 months after storage of residual LBC specimens.
Conclusion: Residual LBC specimens collected from FNA of the breast were verified to carry high-quality DNA and could serve as an alternate source for genetic analysis.
期刊介绍：
With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.
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