小鼠岛叶皮层各亚区神经元和突触特性的性别差异

IF 4.9 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM Biology of Sex Differences Pub Date : 2024-04-01 DOI:10.1186/s13293-024-00593-4
Daniela Iezzi, Alba Cáceres-Rodríguez, Benjamin Strauss, Pascale Chavis, Olivier J Manzoni
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引用次数: 0

摘要

背景:岛叶皮层(IC)在处理感知间信息和情感信息方面发挥着关键作用,为行为和认知方面的性别差异提供了启示。岛叶皮层由两个不同的亚区组成:前岛叶皮层(aIC)和后岛叶皮层(pIC),前者负责处理情感和社交信号,后者则专门处理内感知和痛觉。岛叶皮层内的锥体投射神经元整合多模态感觉输入,影响行为和认知。尽管以前的研究侧重于神经元连接性和转录组学,但在了解锥体神经元在不同亚区域和不同性别之间的特征方面还存在差距:方法:牺牲成年雌雄 C57Bl/6J 小鼠,收集含有 IC 的组织,进行体外切片电生理学记录,研究 aIC 和 pIC 亚区域内突触可塑性和传递的基线性别差异:男性和女性的 aIC 和 pIC 神经元之间存在明显差异:与 pIC 神经元相比,aIC 神经元表现出更大的尺寸、更高的超极化和更高的流变基等显著特征。此外,我们还观察到神经元兴奋性的变化与性别有关,男性 pIC 神经元的兴奋性水平高于女性。我们还发现雌雄小鼠的兴奋性和抑制性突触活动以及兴奋和抑制之间的平衡存在特定区域差异。与pIC相比,成年雌性小鼠在aIC表现出更大的突触强度和最大反应。最后,雄性小鼠的突触长期电位发生在两个亚区域,但雌性小鼠的突触长期电位发生在 aIC:我们得出的结论是,IC 亚区的突触可塑性和兴奋传递存在性别差异,IC 锥体神经元在性别上的不同特性可能会导致男性和女性在行为和认知上的差异。
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Sexual differences in neuronal and synaptic properties across subregions of the mouse insular cortex.

Background: The insular cortex (IC) plays a pivotal role in processing interoceptive and emotional information, offering insights into sex differences in behavior and cognition. The IC comprises two distinct subregions: the anterior insular cortex (aIC), that processes emotional and social signals, and the posterior insular cortex (pIC), specialized in interoception and perception of pain. Pyramidal projection neurons within the IC integrate multimodal sensory inputs, influencing behavior and cognition. Despite previous research focusing on neuronal connectivity and transcriptomics, there has been a gap in understanding pyramidal neurons characteristics across subregions and between sexes.

Methods: Adult male and female C57Bl/6J mice were sacrificed and tissue containing the IC was collected for ex vivo slice electrophysiology recordings that examined baseline sex differences in synaptic plasticity and transmission within aIC and pIC subregions.

Results: Clear differences emerged between aIC and pIC neurons in both males and females: aIC neurons exhibited distinctive features such as larger size, increased hyperpolarization, and a higher rheobase compared to their pIC counterparts. Furthermore, we observed variations in neuronal excitability linked to sex, with male pIC neurons displaying a greater level of excitability than their female counterparts. We also identified region-specific differences in excitatory and inhibitory synaptic activity and the balance between excitation and inhibition in both male and female mice. Adult females demonstrated greater synaptic strength and maximum response in the aIC compared to the pIC. Lastly, synaptic long-term potentiation occurred in both subregions in males but was specific to the aIC in females.

Conclusions: We conclude that there are sex differences in synaptic plasticity and excitatory transmission in IC subregions, and that distinct properties of IC pyramidal neurons between sexes could contribute to differences in behavior and cognition between males and females.

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来源期刊
Biology of Sex Differences
Biology of Sex Differences ENDOCRINOLOGY & METABOLISM-GENETICS & HEREDITY
CiteScore
12.10
自引率
1.30%
发文量
69
审稿时长
14 weeks
期刊介绍: Biology of Sex Differences is a unique scientific journal focusing on sex differences in physiology, behavior, and disease from molecular to phenotypic levels, incorporating both basic and clinical research. The journal aims to enhance understanding of basic principles and facilitate the development of therapeutic and diagnostic tools specific to sex differences. As an open-access journal, it is the official publication of the Organization for the Study of Sex Differences and co-published by the Society for Women's Health Research. Topical areas include, but are not limited to sex differences in: genomics; the microbiome; epigenetics; molecular and cell biology; tissue biology; physiology; interaction of tissue systems, in any system including adipose, behavioral, cardiovascular, immune, muscular, neural, renal, and skeletal; clinical studies bearing on sex differences in disease or response to therapy.
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