标准实验室条件下蓝贻贝肠道常驻微生物群的元转录组

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Current Research in Biotechnology Pub Date : 2024-01-01 DOI:10.1016/j.crbiot.2024.100208
Tyler W. Griffin , Lisa M. Nigro , Hannah I. Collins , Bridget A. Holohan , J. Evan Ward
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引用次数: 0

摘要

涉及 RNA 测序(RNA-seq)的元转录组学方法是评估栖息在动物组织中转录活跃的微生物基因表达谱的有力工具。双壳软体动物与所有无脊椎动物一样,都是全生物体,是宿主动物与原核和真核共生体相互作用的场所。本通讯描述了在标准实验室条件下蓝贻贝(Mytilus edulis)肠道常驻微生物的元转录组概况。本文所述的八种贻贝均在隔离的微生态系统中饲养了 14 天,然后对其肠道组织进行取样并进行 RNA 序列分析。随后删除与贻贝基因组对齐的 mRNA 读数,并对非宿主读数进行注释,以进行功能和通路分析。在实验室条件下,常驻肠道微生物群表达的基因主要与有氧能量过程有关,其他显著贡献来自代谢和蛋白质加工基因。在通路水平上,常驻肠道微生物群落表达的最丰富的完整通路与有氧细胞呼吸、核苷酸生物合成和分解代谢有关。这些数据代表了来自贻贝肠道的新型基线微生物基因表达信息,对于未来研究贻贝全生物体和双壳贝类微生物生态学至关重要。我们非常鼓励公众检索这些元转录组图谱并对其进行二次分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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The metatranscriptome of resident microbiota in the gut of blue mussels, Mytilus edulis, under standard laboratory conditions

Metatranscriptomic methods involving RNA sequencing (RNA-seq) are powerful tools for evaluating the gene expression profiles of transcriptionally-active microbes that inhabit the tissues of animals. Bivalve molluscs, like all invertebrates, are holobionts and sites of interactions between host animals and both prokaryotic and eukaryotic symbionts. The present communication describes the metatranscriptomic profile of the resident microorganisms that inhabit the gut of blue mussels, Mytilus edulis, under standard laboratory conditions. Each of the eight mussels described herein were housed in isolated microcosms with routine husbandry for 14 days before their gut tissues were sampled and subjected to RNA-seq. Subsequent mRNA reads that aligned to the mussel genome were removed, and the non-host reads were annotated for function and pathway analyses. Under laboratory conditions, the resident gut microbiota expressed genes associated mostly with aerobic energy processes, with other notable contributions from metabolism and protein processing genes. At the pathway level, the most abundant complete pathways expressed in the resident gut microbial communities were related to aerobic cellular respiration, nucleotide biosynthesis, and catabolism. These data represent novel, baseline microbial gene expression information from the gut of mussels, which are crucial for future research examining the mussel holobiont and bivalve microbial ecology. Public retrieval and secondary analyses of these metatranscriptomic profiles are highly encouraged.

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来源期刊
Current Research in Biotechnology
Current Research in Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.70
自引率
3.60%
发文量
50
审稿时长
38 days
期刊介绍: Current Research in Biotechnology (CRBIOT) is a new primary research, gold open access journal from Elsevier. CRBIOT publishes original papers, reviews, and short communications (including viewpoints and perspectives) resulting from research in biotechnology and biotech-associated disciplines. Current Research in Biotechnology is a peer-reviewed gold open access (OA) journal and upon acceptance all articles are permanently and freely available. It is a companion to the highly regarded review journal Current Opinion in Biotechnology (2018 CiteScore 8.450) and is part of the Current Opinion and Research (CO+RE) suite of journals. All CO+RE journals leverage the Current Opinion legacy-of editorial excellence, high-impact, and global reach-to ensure they are a widely read resource that is integral to scientists' workflow.
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