带分离芽的发芽豌豆种子在食品生产中的潜在用途

Yu. I. Matveev, E. V. Averyanova
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摘要

在芽形成的过程中,种子会发生一系列生化转化,形成具有生物活性、更高溶解度和生物利用度的大分子中间代谢物,这些物质在各种食品加工应用中具有吸引力。这项研究的目的是评估从发芽的豌豆种子中分离出芽后残留产品的特性,以及调查将其用于食品生产的可能性。作为研究对象,以 Temp、Sofia 和 Spartak 品种为例,考虑了豆科豌豆蛋白的发芽时间、含量、结构和蛋白水解深度等数据。豆芽分离后豆科植物分解产物的组成主要由蛋白质边缘的分解产物决定。根据豆蛋白的结构特征及其蛋白分解方向计算出的依赖关系,确定芽形成时间为 98 小时,与实验数据(96 小时)相符。从发芽的豌豆种子中分离出芽后得到的产品,其蛋白质和肽的含量与从成熟豌豆中得到的面粉有很大不同。这不仅是由于淀粉的水解,还由于蛋白质的逐步水解,导致短链肽的含量增加,增加了面粉中蛋白质成分的溶解度,但在用作各种食品的添加剂时,特别是在液体稠度方面,还需要对其浓度进行适当的修正。
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Potential uses of sprouted pea seeds with separated sprouts in food production
In the process of sprout formation, a seed undergoes a number of biochemical transformations, forming intermediate metabolites of macromolecules that offer biological activity, higher solubility and bioavailability, which are attractive for use in various food processing applications. The purpose of the study was to evaluate the properties of the product that remains following the separation of sprouts from sprouted pea seeds, as well as investigating the possibility of its use in food production. As the objects of the study, data on the germination time,content, structure and depth of proteolysis of legumin pea protein were considered on the example of Temp, Sofia, and Spartak varieties. The composition of the decomposition products of legumin following the separation of the sprout is mainly determined by the decomposition products of the protein edges. According to the calculated dependence based on the structural characteristics of the legumin protein and the direction of its proteolysis, a sprout formation time equal to 98 hours was determined, which corresponds to the experimental data (96 hours). The product obtained from sprouted pea seeds following the separation of sprouts differs significantly in protein and peptide content from flour obtained from ripe peas. This is due not only to the hydrolysis of starch, but also to the step-by-step hydrolysis of protein, which leads to an increase in the content of short-chain peptides, increasing the solubility of the protein component of the flour, but also requiring appropriate correction of their concentration when used as additives to various food products, especially in terms of liquid consistency.
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