某些发乳成分对人体头皮菌群密度的影响

Okwelle, A.A., Amadi-Ikpa, C.N.
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引用次数: 0

摘要

这项研究评估了哈科特港部分地区某些品牌的发膏(编码为 MBB 和 SDR)对人体头皮菌群密度的影响。20 名自愿者在使用编码发膏前后对头皮进行了无菌交换采集。头皮样本采用标准微生物学程序在无菌营养琼脂、甘露醇盐琼脂、麦康凯琼脂和沙保露脱蔗糖琼脂培养基上进行培养。将所有接种的培养基平板在各自要求的温度下培养,然后对培养出的菌落进行计数。结果显示,在使用 SDR 发膏前后,异养菌的数量分别为 9.8 x 103 和 9.3 x 103CFU/ml,而在使用 MBB 头发志愿者前后,异养菌的数量分别为 1.05 x 104 和 1.07 x 104CFU/ml。同样,在使用 SDR 发膏前后,从 MBB 头发中获得的葡萄球菌计数分别为 4.5 x 103 和 3.7 x 103CFU/ml,而在使用 SDR 发膏前后,从 MBB 头发中获得的葡萄球菌计数分别为 6.2 x 103 和 5 x 102 CFU/ml。共分离出 53 个细菌,其中有两个细菌属,即表皮葡萄球菌和痢疾杆菌:表皮葡萄球菌、金黄色葡萄球菌和一种名为克鲁塞念珠菌的真菌。在 SDR 样品中,金黄色葡萄球菌和表皮葡萄球菌的出现率分别为 12%和 56%。在使用 MBB 发膏的志愿者中,表皮葡萄球菌和金黄色葡萄球菌的感染率分别为 14.8% 和 7.4%。因此,观察到 SDR 发膏对人体头皮有负面影响。SDR 发膏大大减少了头皮中葡萄球菌的正常菌群,这可能导致机会性感染的发生。这项研究建议,由于发膏的成分,SDR 发膏的使用者在使用发膏时要多加注意。
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Impact of Some Hair Cream Compositions on the Flora Density of Human Scalp
The study assessed the effect of some brands of hair creams coded MBB and SDR on the flora density of the human scalp in parts of Port Harcourt. Twenty volunteered persons subjected their scalp aseptically; for swap collection before and after application of the coded hair creams. Standard microbiological procedure was adopted to culture the scalp samples on prepared sterile nutrient agar, mannitol salt agar, MacConkey agar and Sabouraud destrose agar media. All the inoculated culture media plates were incubated in their respective temperature requirements and after which colonies that developed were counted. The result showed heterotrophic bacteria counts of 9.8 x 103 and 9.3 x 103CFU/ml for before and after application of SDR hair cream, while counts of 1.05 x 104 and 1.07 x 104CFU/ml were obtained from MBB hair volunteers before and after application respectively. Similarly, a Staphylococcal count of 4.5 x 103 and 3.7 x 103CFU/ml were obtained before and after application of SDR hair cream, while counts of 6.2 x 103 and 5 x 102 CFU/ml were obtained from MBB hair before and after application respectively. A total of 53 isolates were recovered from which two bacteria genera namely: Staphylococcus epidermidis, Staphylococcus aureus and a fungi genera named Candida krusei were identified. Staphylococcus aureus and Staphylococcus epidermidis were noted with a 12% and 56% occurrence respectively, in sample SDR volunteers. MBB cream volunteers, recovered 14.8 and 7.4 % frequency occurrence for Staphylococcus epidermidis and Staphylococcus aureus respectively. Thus, a negative effect of hair cream SDR on the human scalp was observed. The SDR hair cream significantly, reduced the Staphylococcal normal flora of the scalp, which can lead to the development of opportunistic infection. The study recommends that users of SDR cream to be mindful in application due to creams composition.
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