Koksal Gundogdu, Özgen Kılıç Erkek, G. Gündoğdu, Dilek Sayın, Gülçin Abban Mete
{"title":"通过 TGF-Beta/Smad 通路研究丝胶对大鼠实验性膝骨关节炎模型的疗效","authors":"Koksal Gundogdu, Özgen Kılıç Erkek, G. Gündoğdu, Dilek Sayın, Gülçin Abban Mete","doi":"10.17094/vetsci.1415498","DOIUrl":null,"url":null,"abstract":"OBJECTIVE: This study investigates the therapeutic efficacy of sericin in rats with knee osteoarthritis (KOA) induced with monosodium iodoacetate (MIA), focusing on evaluating its effectiveness through the TGF-β/Smad pathway. \nMATERIALS AND METHODS: The KOA model was established by injecting MIA into the knee joint and the rats were randomly divided into three groups:group 1 (control), group 2 (KOA control), and group 3 (KOA+sericin). Sericin was administered intratendinously to rats on days 1,7,14, and 21 (50 µL,0.8 g/kg/mL). After 21 days, the rats were sacrificed, and serum samples were analyzed using the ELISA method to measure TGF-β1, Smad2, and CTGF levels. Additionally, knee joint samples underwent histopathological evaluations with hematoxylin-eosin staining and immunohistochemical assessment using TGF-β1 and Smad2/3 antibodies. \nRESULTS: Serum TGF-β1 and CTGF levels were significantly increased in group 2 vs. group 1 (P","PeriodicalId":104074,"journal":{"name":"Veterinary Sciences and Practices","volume":"19 11","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation of The Efficacy of Sericin in Experimental Knee Osteoarthritis Model in Rats through the TGF-Beta/Smad Pathway\",\"authors\":\"Koksal Gundogdu, Özgen Kılıç Erkek, G. Gündoğdu, Dilek Sayın, Gülçin Abban Mete\",\"doi\":\"10.17094/vetsci.1415498\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"OBJECTIVE: This study investigates the therapeutic efficacy of sericin in rats with knee osteoarthritis (KOA) induced with monosodium iodoacetate (MIA), focusing on evaluating its effectiveness through the TGF-β/Smad pathway. \\nMATERIALS AND METHODS: The KOA model was established by injecting MIA into the knee joint and the rats were randomly divided into three groups:group 1 (control), group 2 (KOA control), and group 3 (KOA+sericin). Sericin was administered intratendinously to rats on days 1,7,14, and 21 (50 µL,0.8 g/kg/mL). After 21 days, the rats were sacrificed, and serum samples were analyzed using the ELISA method to measure TGF-β1, Smad2, and CTGF levels. Additionally, knee joint samples underwent histopathological evaluations with hematoxylin-eosin staining and immunohistochemical assessment using TGF-β1 and Smad2/3 antibodies. \\nRESULTS: Serum TGF-β1 and CTGF levels were significantly increased in group 2 vs. group 1 (P\",\"PeriodicalId\":104074,\"journal\":{\"name\":\"Veterinary Sciences and Practices\",\"volume\":\"19 11\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Sciences and Practices\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17094/vetsci.1415498\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Sciences and Practices","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17094/vetsci.1415498","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Investigation of The Efficacy of Sericin in Experimental Knee Osteoarthritis Model in Rats through the TGF-Beta/Smad Pathway
OBJECTIVE: This study investigates the therapeutic efficacy of sericin in rats with knee osteoarthritis (KOA) induced with monosodium iodoacetate (MIA), focusing on evaluating its effectiveness through the TGF-β/Smad pathway.
MATERIALS AND METHODS: The KOA model was established by injecting MIA into the knee joint and the rats were randomly divided into three groups:group 1 (control), group 2 (KOA control), and group 3 (KOA+sericin). Sericin was administered intratendinously to rats on days 1,7,14, and 21 (50 µL,0.8 g/kg/mL). After 21 days, the rats were sacrificed, and serum samples were analyzed using the ELISA method to measure TGF-β1, Smad2, and CTGF levels. Additionally, knee joint samples underwent histopathological evaluations with hematoxylin-eosin staining and immunohistochemical assessment using TGF-β1 and Smad2/3 antibodies.
RESULTS: Serum TGF-β1 and CTGF levels were significantly increased in group 2 vs. group 1 (P
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