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Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq 伊拉克尼尼微省绵羊李斯特流产的分子检测
Pub Date : 2024-03-27 DOI: 10.17094/vetsci.1415509
H. Mohammad, Maab Ibrahim Al-farwachi, Balsam Rasheed
Listeriosis is a important abortive diseases in sheep , and is considered one of the most risky bacterial zoonotic disease worldwide.The study was carried out in 50 sheep flocks placed in the Nineveh governorate, Iraq during November and December 2022, a total of 300 specimens of blood , abomasum content and brain ( 100 each)were collected from local breed ovine aborted fetues(in the last stage of gestation) were obtained to be tested for molecular detection of Listeria monocytogenes . L monocytogenes DNA was detected in a total of 61(20.3%) specimens , distributed as: 35(57.4%) ,15(24.6% and 11(18.0%) strains from fetal brain, abomasum and blood specimens respectively using direct genus-specific conventional polymerase chain reaction (prfA gene) C- PCR. Two L. monocytogenes strains ( HMB1 listeriolysin, HMB 2 listeriolysin ) deposited in GenBank under accession numbers LC769365.1,and LC769366.1. All L. monocytogenes strains were positive for three genes (InlJ, InlA, and hlyA) except actA gene was detected in 46 (75.4%) strains. In conclusion , L. monocytogenes is one of the important causal agents of abortion in sheep flocks in Nineveh province , Iraq, and most of the brain specimens were positive for listeric infection compared to the other specimens.
该研究于2022年11月至12月期间在伊拉克尼尼微省的50个羊群中进行,从当地品种的绵羊流产胎儿(处于妊娠的最后阶段)中采集了300份血液、腹腔内容物和脑标本(各100份),用于李斯特菌的分子检测。共有 61 份(20.3%)标本检测到单核细胞增多性李斯特菌 DNA,其分布情况如下使用直接属特异性传统聚合酶链反应(prfA 基因)C- PCR 技术,分别从胎儿脑、腹腔和血液标本中检测到 35 株(57.4%)、15 株(24.6%)和 11 株(18.0%)单核细胞增多性李斯特菌。两株单核细胞增生症菌株(HMB1 李斯特溶蛋白、HMB 2 李斯特溶蛋白)已存入 GenBank,登录号分别为 LC769365.1 和 LC769366.1。除了在 46 株(75.4%)菌株中检测到 actA 基因外,所有单核细胞增生梭菌菌株的三个基因(InlJ、InlA 和 hlyA)均呈阳性。总之,单增李斯特菌是导致伊拉克尼尼微省羊群流产的重要病原体之一,与其他标本相比,大多数脑标本对李斯特菌感染呈阳性反应。
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引用次数: 0
Investigation of The Efficacy of Sericin in Experimental Knee Osteoarthritis Model in Rats through the TGF-Beta/Smad Pathway 通过 TGF-Beta/Smad 通路研究丝胶对大鼠实验性膝骨关节炎模型的疗效
Pub Date : 2024-03-27 DOI: 10.17094/vetsci.1415498
Koksal Gundogdu, Özgen Kılıç Erkek, G. Gündoğdu, Dilek Sayın, Gülçin Abban Mete
OBJECTIVE: This study investigates the therapeutic efficacy of sericin in rats with knee osteoarthritis (KOA) induced with monosodium iodoacetate (MIA), focusing on evaluating its effectiveness through the TGF-β/Smad pathway. MATERIALS AND METHODS: The KOA model was established by injecting MIA into the knee joint and the rats were randomly divided into three groups:group 1 (control), group 2 (KOA control), and group 3 (KOA+sericin). Sericin was administered intratendinously to rats on days 1,7,14, and 21 (50 µL,0.8 g/kg/mL). After 21 days, the rats were sacrificed, and serum samples were analyzed using the ELISA method to measure TGF-β1, Smad2, and CTGF levels. Additionally, knee joint samples underwent histopathological evaluations with hematoxylin-eosin staining and immunohistochemical assessment using TGF-β1 and Smad2/3 antibodies. RESULTS: Serum TGF-β1 and CTGF levels were significantly increased in group 2 vs. group 1 (P
目的:本研究探讨了丝裂霉素对碘乙酸钠(MIA)诱导的膝骨关节炎(KOA)大鼠的疗效,重点是评估其通过 TGF-β/Smad 通路的有效性。材料与方法:通过向膝关节注射 MIA 建立 KOA 模型,并将大鼠随机分为三组:第 1 组(对照组)、第 2 组(KOA 对照组)和第 3 组(KOA+丝裂霉素组)。在第 1、7、14 和 21 天给大鼠鞘内注射丝裂霉素(50 µL,0.8 g/kg/mL)。21 天后,大鼠被处死,使用 ELISA 方法分析血清样本,以测量 TGF-β1、Smad2 和 CTGF 水平。此外,膝关节样本经苏木精-伊红染色进行组织病理学评估,并使用 TGF-β1 和 Smad2/3 抗体进行免疫组化评估。结果:与第一组相比,第二组的血清 TGF-β1 和 CTGF 水平明显升高(P<0.05)。
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引用次数: 0
A Preliminary Study on the Investigation of Learning Ability of Arabian Horses through Behavioural Tests 通过行为测试调查阿拉伯马学习能力的初步研究
Pub Date : 2024-02-13 DOI: 10.17094/vetsci.1416899
S. Danışan, Özlem Gücüyener Hacan
Knowing the behavioural responses of horses while planning the training processes is crucial in constituting the appropriate training program. For this purpose, behavioural tests are used. 2-8 years old, fifteen female Arabian horses participated in the study. Reactivity to Human and Fear Tests were applied within the scope of Behavioural Tests. Significant relationships (p
在计划训练过程时,了解马匹的行为反应对于制定适当的训练计划至关重要。为此,我们使用了行为测试。参与研究的是 15 匹 2 至 8 岁的雌性阿拉伯马。在行为测试中采用了对人的反应和恐惧测试。结果表明,这两项测试之间存在显著的关系(p
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引用次数: 0
Effectiveness of Hypochlorous Acid in Cats with Bacterial Cystitis 次氯酸对猫细菌性膀胱炎的疗效
Pub Date : 2023-12-20 DOI: 10.5152/vetscipract.2023.23172
Serpil Elmaci, Sercan Hüseyin Bayendur, Abuzer Acar
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引用次数: 0
Comparison of a Hybrid Intramedullary Pin with External Fixation Procedure and a Tape Splint on Tibiotarsal Fractures in Budgerigars (Melopsittacus undulatus): A Retrospective Study 混合髓内钉外固定与带式夹板治疗虎牙胫跖骨折的回顾性研究
Pub Date : 2023-08-17 DOI: 10.5152/vetscipract.2023.23102
M. Senocak, L. Yanmaz, E. Doğan, S. Okur, Ugur Ersoz, F. Turgut, Ayse GOLGELI BEDIR, Omer Tarik Orhun, Y. Kocaman, Z. Okumuş
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引用次数: 0
The Effects of Dietary Supplementation with Origanum onites Essential Oil on Growth Performance, Some Blood Parameters, Jejunal Villus Height, and Meat Quality in Broiler Chickens 饲粮中添加牛头草精油对肉鸡生长性能、部分血液指标、空肠绒毛高度和肉品质的影响
Pub Date : 2023-08-17 DOI: 10.5152/vetscipract.2023.23107
Huseyin Gurkan Sarac, M. A. Yoruk
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引用次数: 0
Molecular and Pathological Survey of Bovine Viral Diarrhea Virus in the Testis of Bulls 公牛睾丸中牛病毒性腹泻病毒的分子病理学研究
Pub Date : 2023-08-17 DOI: 10.5152/vetscipract.2023.22056
M. Ozkaraca, M. Timurkan, Y. Saglam, S. Ozdemir, H. Aydın, S. Altun
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引用次数: 0
General Beekeeping Structure in Sivas, Turkiye 土耳其锡瓦斯的一般养蜂结构
Pub Date : 2023-08-17 DOI: 10.5152/vetscipract.2023.22055
Erhan Arslan, Metin Bayraktar
{"title":"General Beekeeping Structure in Sivas, Turkiye","authors":"Erhan Arslan, Metin Bayraktar","doi":"10.5152/vetscipract.2023.22055","DOIUrl":"https://doi.org/10.5152/vetscipract.2023.22055","url":null,"abstract":"","PeriodicalId":104074,"journal":{"name":"Veterinary Sciences and Practices","volume":"200 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116435331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hypoglycemic and Hypolipidemic Activities of Aqueous Root Extract of Senna alata in Alloxan-Induced Diabetic Wistar Rats 番泻根水提物对四氧嘧啶诱导的糖尿病Wistar大鼠的降血糖和降血脂活性
Pub Date : 2023-08-17 DOI: 10.5152/vetscipract.2023.23127
S. Attama, N. Okwelum, Paul F. Egunleti, S. C. David, Timothy U. Obetta, Michael J. Aguiyi
{"title":"Hypoglycemic and Hypolipidemic Activities of Aqueous Root Extract of Senna alata in Alloxan-Induced Diabetic Wistar Rats","authors":"S. Attama, N. Okwelum, Paul F. Egunleti, S. C. David, Timothy U. Obetta, Michael J. Aguiyi","doi":"10.5152/vetscipract.2023.23127","DOIUrl":"https://doi.org/10.5152/vetscipract.2023.23127","url":null,"abstract":"","PeriodicalId":104074,"journal":{"name":"Veterinary Sciences and Practices","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127401545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression of Zonula Occludens-1 and Claudin-1 Proteins in Japanese Quails Testis 日本鹌鹑睾丸Occludens-1和Claudin-1蛋白的表达
Pub Date : 2023-07-20 DOI: 10.5152/vetscipract.2023.22070
I. Undag, H. H. Donmez
{"title":"Expression of Zonula Occludens-1 and Claudin-1 Proteins in Japanese Quails Testis","authors":"I. Undag, H. H. Donmez","doi":"10.5152/vetscipract.2023.22070","DOIUrl":"https://doi.org/10.5152/vetscipract.2023.22070","url":null,"abstract":"","PeriodicalId":104074,"journal":{"name":"Veterinary Sciences and Practices","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121316056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Veterinary Sciences and Practices
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