基于酶规划的生物分级作为环境监测和食品安全初步筛选方法的应用系统综述环境监测和食品安全

M. K. Sabullah, R. Abdullah, Roslina Jawan, Lucky Goh Poh Wah, Hartinie Marbawi, Syed Umar Faruq Syed Najmuddin, Jualang Azlan Gansau, Mohd Yunus Syukor
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引用次数: 0

摘要

本文综述了基于酶抑制的生物传感器开发过程中的最新发现。由于生物传感器具有出色的选择性和灵敏度,因此是传统分析方法的重要替代方法;传统分析方法只依赖仪器数据的生成,而不进行任何初步筛选。从根本上说,生物传感器能够将生物活性转化为可量化的信号。这些基于酶抑制的生物传感器在环境安全、食品安全和临床分析领域有着广泛的应用,因为含有重金属和农药的有毒物质是酶最有效的抑制剂。本文旨在探讨基于葡萄糖氧化酶、尿素酶、酪氨酸酶、胆碱酯酶等酶的抑制作用的生物传感器所使用的方法以及对各种抑制剂的灵敏度。
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ULASAN SISTEMATIK KEATAS PENGAPLIKASIAN BIOPENDERIA BERASASKAN PERENCATAN ENZIM SEBAGAI KAEDAH SARINGAN AWAL UNTUK PEMANTAUAN ALAM SEKITAR DAN KESELAMATAN MAKANANEMANTAUAN ALAM SEKITAR DAN KESELAMATAN MAKANAN
In this article, the latest discoveries in the development of biosensors based on enzyme inhibition are reviewed. Due to their excellent selectivity and sensitivity, they represent a significant alternative method to conventional analytical methods; which is a method of analysis that only relies on the generation of instrumentation data without any preliminary screening. Basically, biosensors are able to convert biological activity into a quantifiable signal. These enzyme inhibition-based biosensors have a wide range of applications in the fields of environmental safety, food safety, and clinical analysis since toxic substances containing heavy metals and pesticides are the most effective inhibitors of enzymes. This paper is aimed at exploring the methods used and the sensitivity to various inhibitors for biosensors based on the inhibition of enzymes such as glucose oxidase, urease, tyrosinase, cholinesterase, and other enzymes.
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