用高效液相色谱法定量测定富都省采集的地黄 19 号品种根中的梓酚含量

Thanh Loan Pham
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摘要

地黄 19 根中的有效成分梓酚是一种重要的化学成分。梓醇具有降血糖、利尿和通便的作用,可用于辅助治疗糖尿病、缺血性脑病和痴呆症,并被越南药典 V(2017 年)规定为检查药材质量的标志物。本研究旨在开发一种高效液相色谱法(HPLC)定量检测地黄 19 号品种根中梓醇含量的程序,以评估药材质量。研究结果选择了合适的色谱条件:Gemini C18色谱柱(250x4.6 mm,5 μm),检测波长为210 nm,流动相为乙腈-磷酸0.1%,流速为0.6 ml/min。峰面积与梓醇浓度呈较强的线性关系,r=0.9999,Y=21887X + 26213。该方法具有良好的准确性和重复性,相对标准偏差(RSD)小于 2%。糯稻品种 19 的根中梓醇的定量结果为 1.25%。该高效液相色谱法适用于定量检测 "芸苔素 19 "根中的梓酚含量。
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Quantification of catalpol in root of Rehmannia glutinosa varieties 19 collected in Phu Tho province by high-performance liquid chromatography
The active ingredient catalpol in the Rehmannia glutinosavariety 19 roots is an important chemical component. Catalpol has hypoglycemia, diuretic, and laxative effects; it is used to support the treatment of diabetes, ischemic cerebral, and dementia, and it is regulated by Vietnamese Pharmacopoeia V (2017) as a marker to check the quality of medicinal herbs. This study aimed to develop a procedure for quantifying catalpol in the root of Rehmannia glutinosa varieties 19 by high-performance liquid chromatography (HPLC) to evaluate the quality of medicinal herbs. The research results have selected suitable chromatographic conditions using the Gemini C18 column (250x4.6 mm, 5 μm), detector at 210 nm, mobile phase acetonitrile - phosphoric acid 0.1%, and flow rate at 0.6 ml/min. The peak area and catalpol concentration have a strong linear correlation with r=0.9999, Y = 21887X + 26213. The procedure has good accuracy and repeatability with a relative standard deviation (RSD) <2%. Quantitative results of catalpol in the root of the R. glutinosa varieties 19 reached 1.25%. The HPLC method is suitable for quantifying catalpol in the root of R. glutinosa varieties 19.
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