P. Meenaloshini , A. Thirumurugan , P.K. Mohan , T. Senthil Kumar
{"title":"从 Crateva religiosa G.Forst.树皮水醇提取物对人类卵巢癌细胞的抗癌作用","authors":"P. Meenaloshini , A. Thirumurugan , P.K. Mohan , T. Senthil Kumar","doi":"10.1016/j.hermed.2024.100870","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p>Medical research on cancer treatment is getting worldwide attention in the present day. The usage of plant sources for cancer treatment is getting much consideration because of their low toxic nature, easy availability, and with fewer side effects. This study illustrates the anticancer potential of <em>Crateva religiosa</em> G. Forst. extract against human ovarian cancer cells (PA-1).</p></div><div><h3>Methods</h3><p>Cell viability was determined using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, and acridine orange/ethidium bromide staining techniques were used to evaluate the cell death and anticancer activity. Additionally, antioxidant activities such as hydroxyl-radical scavenging, 2,2-diphenylpicrylhydrazyl, ferric reducing ability of plasma, and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate<sup>+</sup> were carried out. Moreover, phytochemical profiling and their quantification were also performed.</p></div><div><h3>Results</h3><p>The hydroalcoholic bark extract of <em>C. religiosa</em> has a strong anticancer prospective against PA-1 with a half-inhibitory concentration value of 33.27 μg/ml. The antioxidant potential of the extract showed potent activity on various antioxidants. The phytochemical profiling of the extract showed the presence of various phytochemicals and the quantification indicated the presence of a total phenol content of 2.1 ± 0.09 mg Gallic acid equivalent/g, total flavonoid content of 1.706 ± 0.01 mg CE/g, total alkaloid content of 1.237 ± 0.03 mg AE/g, and total terpenoid content of 1.004 ± 0.06 mg UAE/g.</p></div><div><h3>Conclusions</h3><p>The current findings of the study suggest the role of <em>C. religiosa</em> bark against human ovarian cancer cells (PA-1) and could be a potential drug source for the discovery of anticancer agents.</p></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"45 ","pages":"Article 100870"},"PeriodicalIF":2.2000,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In Vitro Anticancer Efficacy of Hydroalcoholic Extract From Crateva religiosa G. Forst. Bark on Human Ovarian Cancer Cells\",\"authors\":\"P. Meenaloshini , A. Thirumurugan , P.K. Mohan , T. Senthil Kumar\",\"doi\":\"10.1016/j.hermed.2024.100870\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p>Medical research on cancer treatment is getting worldwide attention in the present day. The usage of plant sources for cancer treatment is getting much consideration because of their low toxic nature, easy availability, and with fewer side effects. This study illustrates the anticancer potential of <em>Crateva religiosa</em> G. Forst. extract against human ovarian cancer cells (PA-1).</p></div><div><h3>Methods</h3><p>Cell viability was determined using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, and acridine orange/ethidium bromide staining techniques were used to evaluate the cell death and anticancer activity. Additionally, antioxidant activities such as hydroxyl-radical scavenging, 2,2-diphenylpicrylhydrazyl, ferric reducing ability of plasma, and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate<sup>+</sup> were carried out. Moreover, phytochemical profiling and their quantification were also performed.</p></div><div><h3>Results</h3><p>The hydroalcoholic bark extract of <em>C. religiosa</em> has a strong anticancer prospective against PA-1 with a half-inhibitory concentration value of 33.27 μg/ml. The antioxidant potential of the extract showed potent activity on various antioxidants. The phytochemical profiling of the extract showed the presence of various phytochemicals and the quantification indicated the presence of a total phenol content of 2.1 ± 0.09 mg Gallic acid equivalent/g, total flavonoid content of 1.706 ± 0.01 mg CE/g, total alkaloid content of 1.237 ± 0.03 mg AE/g, and total terpenoid content of 1.004 ± 0.06 mg UAE/g.</p></div><div><h3>Conclusions</h3><p>The current findings of the study suggest the role of <em>C. religiosa</em> bark against human ovarian cancer cells (PA-1) and could be a potential drug source for the discovery of anticancer agents.</p></div>\",\"PeriodicalId\":56077,\"journal\":{\"name\":\"Journal of Herbal Medicine\",\"volume\":\"45 \",\"pages\":\"Article 100870\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-03-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Herbal Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2210803324000277\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Herbal Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2210803324000277","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
In Vitro Anticancer Efficacy of Hydroalcoholic Extract From Crateva religiosa G. Forst. Bark on Human Ovarian Cancer Cells
Introduction
Medical research on cancer treatment is getting worldwide attention in the present day. The usage of plant sources for cancer treatment is getting much consideration because of their low toxic nature, easy availability, and with fewer side effects. This study illustrates the anticancer potential of Crateva religiosa G. Forst. extract against human ovarian cancer cells (PA-1).
Methods
Cell viability was determined using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, and acridine orange/ethidium bromide staining techniques were used to evaluate the cell death and anticancer activity. Additionally, antioxidant activities such as hydroxyl-radical scavenging, 2,2-diphenylpicrylhydrazyl, ferric reducing ability of plasma, and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate+ were carried out. Moreover, phytochemical profiling and their quantification were also performed.
Results
The hydroalcoholic bark extract of C. religiosa has a strong anticancer prospective against PA-1 with a half-inhibitory concentration value of 33.27 μg/ml. The antioxidant potential of the extract showed potent activity on various antioxidants. The phytochemical profiling of the extract showed the presence of various phytochemicals and the quantification indicated the presence of a total phenol content of 2.1 ± 0.09 mg Gallic acid equivalent/g, total flavonoid content of 1.706 ± 0.01 mg CE/g, total alkaloid content of 1.237 ± 0.03 mg AE/g, and total terpenoid content of 1.004 ± 0.06 mg UAE/g.
Conclusions
The current findings of the study suggest the role of C. religiosa bark against human ovarian cancer cells (PA-1) and could be a potential drug source for the discovery of anticancer agents.
期刊介绍:
The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.