高效水蚤生物絮凝的生长动力学模型

Q4 Agricultural and Biological Sciences Journal of Tropical Biodiversity and Biotechnology Pub Date : 2024-02-26 DOI:10.22146/jtbb.82196
Amalia Rahmawati, Irma Rohmawati, Istini Nurafifah, B. Sadewo, E. Suyono
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引用次数: 0

摘要

生物絮凝是一种利用细菌和微藻等絮凝剂的收获技术。其优点是无需添加化学絮凝剂。由于细菌需要特定的培养基,因此微藻絮凝剂更为有效。本研究使用 Anabaena sp. 从 Glagah 联合体中收集脂肪、蛋白质和碳水化合物。这些微藻在 300 毫升的 Bold Basal Medium 培养基中生长了 8 天。收获当天,絮凝微藻(Anabaena sp.)和非絮凝微藻(Glagah)混合以完成絮凝。收获当天,以 1:1、0.5:1 和 0.25:1 的比例将 Anabaena sp.与 Glagah 联合体混合,观察各项参数。利用波长为 750 纳米的分光光度计计算沉淀比例。使用 Bligh 和 Dyer 测量脂质。采用硫酸苯酚技术计算碳水化合物的含量。蛋白质采用布拉德福德法进行定量。生物絮凝百分比和碳水化合物含量的最佳比例为 0.25:1。脂质和蛋白质含量的最佳比例为 1:1。
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Growth Kinetic Modelling of Efficient Anabaena sp. Bioflocculation
Bioflocculation is a harvesting technique that employs flocculant agents such as bacteria and microalgae. The benefit is the absence of a chemical-added flocculant. Because bacteria need a particular medium, microalgae flocculant agents are more effective. This study used Anabaena sp. to collect fat, protein, and carbohydrates from the Glagah consortium. Three replications of those microalgae were grown in 300 ml of Bold Basal Medium culture for eight days. On the day of harvest, flocculant microalgae (Anabaena sp.) and non-flocculant microalgae (Glagah) were combined to accomplish flocculation. On the day of harvest, parameters were observed by combining Anabaena sp. with the Glagah consortium in the ratios 1: 1, 0.5: 1, and 0.25: 1. There were three times of each parameter test. Utilizing a wavelength of 750 nm, the proportion of precipitation was calculated spectrophotometrically. Bligh and Dyer were used to measure the lipids. The phenol sulfate technique was used to calculate the amount of carbohydrates. By employing the Bradford method, proteins were quantified. Biofocculation percentages and carbohydrate content were optimum on a ratio of 0.25:1. Lipid and protein content were optimum on a ratio of 1:1.
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来源期刊
Journal of Tropical Biodiversity and Biotechnology
Journal of Tropical Biodiversity and Biotechnology Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
1.10
自引率
0.00%
发文量
40
审稿时长
12 weeks
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