A. D. Prawestri, R. S. Rahayu, Wulan Septiningtyas Kurniajati, Sunardi Sunardi, Muhammad Mansur
The study to optimize in vitro propagation of the Indonesian native and critically endangered species, Nepenthes jamban, in order to support the ex-situ conservation efforts has been done. Using Murashige and Skoog (MS) as a basal media, disinfected seeds of N. jamban were germinated on five types of germination media, viz. ¼ MS, ½ MS, MS, ¼ MS+benzyl adenine (BA)+Biotin and MS+BA+Biotin. Afterwards, in vitro shoots with 6-7 leaves were inoculated on growing media, i.e., ¼ MS, ¼ MS 60 (3:1) (MS modification with a higher concentration of nitrogen), and ¼ MS+naphtalene acetic acid (NAA)+BA. The results showed that the germination of N. jamban seeds was slow, indicated by the percentage of germination being less than 20% after 6 months of being planted on germination media. The highest percentage of germination was after the 6th month and the greatest pitcher development at the 10th month were obtained on ¼ MS medium. Furthermore, shoot growth and pitchers development consistently increased for 12 months in ¼ MS 60 (3:1) medium while other media resulted in a decrease in pitcher formation. It seemed that low concentrations of nutrient in the medium proved to be more effective to induce in vitro seed germination and enhance shoot growth which was also supported by higher nitrogen (nitrate) concentration in the medium. This study provides information that supports ex situ conservation action of native and critically endangered Nepenthes species from Indonesia.
该研究旨在优化印尼本土极度濒危物种楠木（Nepenthes jamban）的体外繁殖，以支持异地保护工作。 以 Murashige and Skoog（MS）为基本培养基，将经过消毒的 N. jamban 种子在五种发芽培养基上发芽，即 ¼ MS、½ MS、MS、¼ MS+ 苄基腺嘌呤（BA）+ 生物素和 MS+BA+生物素。然后，将 6-7 片叶的离体芽接种到生长培养基上，即 ¼ MS、¼ MS 60 (3:1)（氮浓度较高的 MS 改性培养基）和 ¼ MS+萘乙酸（NAA）+BA。结果表明，N. jamban 种子发芽缓慢，在发芽培养基上种植 6 个月后，发芽率低于 20%。在 ¼ MS 培养基上，第 6 个月后的发芽率最高，第 10 个月时的球茎发育最好。此外，在 ¼ MS 60（3:1）培养基中，12 个月的新芽生长和菌球发育持续增长，而其他培养基则导致菌球形成减少。事实证明，培养基中低浓度的营养物质似乎能更有效地诱导离体种子萌发和促进幼芽生长，培养基中较高浓度的氮（硝酸盐）也支持这一点。这项研究提供的信息支持了对印尼本地和极度濒危的景天科植物的异地保护行动。
{"title":"In Vitro Seed Germination and Shoot Growth of Nepenthes jamban Chi. C. Lee, Hernawati & Akhriadi, A Unique Pitcher Plant from Indonesia","authors":"A. D. Prawestri, R. S. Rahayu, Wulan Septiningtyas Kurniajati, Sunardi Sunardi, Muhammad Mansur","doi":"10.22146/jtbb.87674","DOIUrl":"https://doi.org/10.22146/jtbb.87674","url":null,"abstract":"The study to optimize in vitro propagation of the Indonesian native and critically endangered species, Nepenthes jamban, in order to support the ex-situ conservation efforts has been done. Using Murashige and Skoog (MS) as a basal media, disinfected seeds of N. jamban were germinated on five types of germination media, viz. ¼ MS, ½ MS, MS, ¼ MS+benzyl adenine (BA)+Biotin and MS+BA+Biotin. Afterwards, in vitro shoots with 6-7 leaves were inoculated on growing media, i.e., ¼ MS, ¼ MS 60 (3:1) (MS modification with a higher concentration of nitrogen), and ¼ MS+naphtalene acetic acid (NAA)+BA. The results showed that the germination of N. jamban seeds was slow, indicated by the percentage of germination being less than 20% after 6 months of being planted on germination media. The highest percentage of germination was after the 6th month and the greatest pitcher development at the 10th month were obtained on ¼ MS medium. Furthermore, shoot growth and pitchers development consistently increased for 12 months in ¼ MS 60 (3:1) medium while other media resulted in a decrease in pitcher formation. It seemed that low concentrations of nutrient in the medium proved to be more effective to induce in vitro seed germination and enhance shoot growth which was also supported by higher nitrogen (nitrate) concentration in the medium. This study provides information that supports ex situ conservation action of native and critically endangered Nepenthes species from Indonesia. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141371210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mulyati, Susy Wijayanti, Annisa Nur Islahi, Anindyanari Rahma Sriyekti Saraswati, Ashfiya Hanif Hasnadewi, Safira Ratri Dwi Setyasari
Ulva lactuca is a macroalgae that contains high nutritional values. The heavy metal contaminants in natural Ulva lactuca needs to be eliminated or decreased using natural agent. The aim of this research was to determine the bioaccumulation of Pb, Cd, Hg, and the impact on liver and gastrointestinal function. Parameters of this research were Hepatosomatic Index (HSI), SGPT levels, SGOT levels, bioaccumulation Pb, Cd, Hg, and histological structure of liver and small intestine. Besides that, the progression of body weight was observed. Twelve female Wistar rats (Rattus norvegicus Berkenhout, 1769) were randomly assigned to three groups: Control, NU (treated with natural Ulva lactuca), and DU (treated with heavy metal decontaminated Ulva lactuca using Averrhoa bilimbi juice).Treatment was carried out orally at a dose of 1000 mg/Kg BW/day for 30 days. Histological structure of rat’s liver and small intestine were prepared after necropsy at the end of this research. Based on results, it can be concluded that there were no significant differences observed in HSI, SGPT, and SGOT levels among the groups. However, there was a tendency for an increase in total bilirubin levels in the decontaminated Ulva lactuca group. Both natural and heavy metal decontaminated Ulva lactuca showed histological damage on liver and small intestine. Bioaccumulation of Cd and Hg in the liver and gastrointestinal tract of rats after consuming decontaminated Ulva lactuca was lower than the natural Ulva lactuca group, but need more observations.
{"title":"Metal Bioaccumulation in Albino Rat Tissues Treated with Decontaminated Sea Lettuce (Ulva lactuca L.)","authors":"M. Mulyati, Susy Wijayanti, Annisa Nur Islahi, Anindyanari Rahma Sriyekti Saraswati, Ashfiya Hanif Hasnadewi, Safira Ratri Dwi Setyasari","doi":"10.22146/jtbb.84533","DOIUrl":"https://doi.org/10.22146/jtbb.84533","url":null,"abstract":"Ulva lactuca is a macroalgae that contains high nutritional values. The heavy metal contaminants in natural Ulva lactuca needs to be eliminated or decreased using natural agent. The aim of this research was to determine the bioaccumulation of Pb, Cd, Hg, and the impact on liver and gastrointestinal function. Parameters of this research were Hepatosomatic Index (HSI), SGPT levels, SGOT levels, bioaccumulation Pb, Cd, Hg, and histological structure of liver and small intestine. Besides that, the progression of body weight was observed. Twelve female Wistar rats (Rattus norvegicus Berkenhout, 1769) were randomly assigned to three groups: Control, NU (treated with natural Ulva lactuca), and DU (treated with heavy metal decontaminated Ulva lactuca using Averrhoa bilimbi juice).Treatment was carried out orally at a dose of 1000 mg/Kg BW/day for 30 days. Histological structure of rat’s liver and small intestine were prepared after necropsy at the end of this research. Based on results, it can be concluded that there were no significant differences observed in HSI, SGPT, and SGOT levels among the groups. However, there was a tendency for an increase in total bilirubin levels in the decontaminated Ulva lactuca group. Both natural and heavy metal decontaminated Ulva lactuca showed histological damage on liver and small intestine. Bioaccumulation of Cd and Hg in the liver and gastrointestinal tract of rats after consuming decontaminated Ulva lactuca was lower than the natural Ulva lactuca group, but need more observations. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"14 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141388293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natasya Adelia Harun, Irwan Lovadi, Rahmawati Rahmawati, Didin Joharudin
The influence of elevation gradient has been investigated across different taxa. However, such studies are scarce for macrofungal communities. This study examined the community structure of Basidiomycota macrofungi across four elevations in Bukit Baka Bukit Raya National Park, Indonesia. Macrofungi were collected from randomly placed five 10 x 10 m plots at each altitude and identified at the genus level. The results showed that there were 32 genera belonging to 20 families. The NMDS ordination and ANOSIM confirmed that macrofungal composition and abundance do not differ between the studied altitudinal ranges.
{"title":"Basidiomycota Macrofungal Communities Across Four Altitudinal Ranges in Bukit Baka Bukit Raya National Park, Indonesia","authors":"Natasya Adelia Harun, Irwan Lovadi, Rahmawati Rahmawati, Didin Joharudin","doi":"10.22146/jtbb.87309","DOIUrl":"https://doi.org/10.22146/jtbb.87309","url":null,"abstract":"The influence of elevation gradient has been investigated across different taxa. However, such studies are scarce for macrofungal communities. This study examined the community structure of Basidiomycota macrofungi across four elevations in Bukit Baka Bukit Raya National Park, Indonesia. Macrofungi were collected from randomly placed five 10 x 10 m plots at each altitude and identified at the genus level. The results showed that there were 32 genera belonging to 20 families. The NMDS ordination and ANOSIM confirmed that macrofungal composition and abundance do not differ between the studied altitudinal ranges. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140390688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. N. Ningtyas, N. A. Aryanti, T. S. S. D. Susilo, Mahmuddin Rahmadana, Ika Yuni Agustin
Javan Langur (T. auratus) is well-known as one of endemic primates from Java, Bali and Lombok Islands. The activities of land clearing, vegetation conversing, wild hunting and illegal wildlife trading are the main causes of the extinction of the Javan Langur. It can be used as an important issue for conservation action by making prediction maps of suitable habitat potential, especially for species facing a high risk of extinction in the wild. We were documenting an information about potential habitat for Javan langur using spatial suitability model in order to provide rigorous information as the basis for conservation activities of Javan langur in TNBTS. We used Landsat-8 TM image and geospatial data to support analysis as a representative of environmental parameters in order to develop the habitat model. We were using maximum entropy (MaxEnt) algorithm refers to Javan langur presence or absence. The results showed that the suitability of the Javan langur habitat in TNBTS has an excellent model accuracy level with an AUC (Area Under the Receiver Operating Characteristics) value of 0.964 and a standard deviation of 0.961. Parameters with the highest response values here are elevation, NDVI (Normalised Difference Vegetation Index) and temperatures.
{"title":"Spatial Modelling Habitat Suitability of Javan Langur (Trachypithecus auratus É. Geoffroy Saint-Hilaire, 1812) in Bromo Tengger Semeru National Park (TNBTS), East Java","authors":"A. N. Ningtyas, N. A. Aryanti, T. S. S. D. Susilo, Mahmuddin Rahmadana, Ika Yuni Agustin","doi":"10.22146/jtbb.76841","DOIUrl":"https://doi.org/10.22146/jtbb.76841","url":null,"abstract":"Javan Langur (T. auratus) is well-known as one of endemic primates from Java, Bali and Lombok Islands. The activities of land clearing, vegetation conversing, wild hunting and illegal wildlife trading are the main causes of the extinction of the Javan Langur. It can be used as an important issue for conservation action by making prediction maps of suitable habitat potential, especially for species facing a high risk of extinction in the wild. We were documenting an information about potential habitat for Javan langur using spatial suitability model in order to provide rigorous information as the basis for conservation activities of Javan langur in TNBTS. We used Landsat-8 TM image and geospatial data to support analysis as a representative of environmental parameters in order to develop the habitat model. We were using maximum entropy (MaxEnt) algorithm refers to Javan langur presence or absence. The results showed that the suitability of the Javan langur habitat in TNBTS has an excellent model accuracy level with an AUC (Area Under the Receiver Operating Characteristics) value of 0.964 and a standard deviation of 0.961. Parameters with the highest response values here are elevation, NDVI (Normalised Difference Vegetation Index) and temperatures. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140391412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Efrilia Mahdilah Nurhidayah, A. Akmaluddin, D. Barianto, Salahuddin Husein, Asep Saripudin
The nannoplankton research was conducted in the MH-2 well, Banggai-Sula Basin, Central Sulawesi. Thirty-four ditch-cutting samples were utilized to observe the Minahaki, Kintom, and Biak Formations. Age determination was carried out using biostratigraphy method and standard procedure for first and last occurrence of nannoplankton biodatum and had an absolute age, widely known as a zone indicator. This study aims to determine the age and nannoplankton biozonation of each geological formation in Banggai-Sula Basin. Based on the biostratigraphic analysis, 39 species from 14 genera with abundance frequencies ranging from rare to abundant were found in the MH-2 well. In this study, new results of the age of Banggai-Sula Basin areMiddle Miocene – Early Middle Pliocene (13,706 – 3,727 Ma), and can identify into six calcareous nannoplankton zones that are more detailed than previous researchers, Discoaster signus zone (NN5), Discoaster exilis zone (NN6-NN7), Discoaster berggrenii zone (NN11), Ceratolithus acustus zone (NN12), Helicosphaera sellii zone (NN13-NN15), and the Discoaster tamalis zone (NN16). Biostratigraphic data also shows new information for the first time, the absence of three zones from zone NN8 to zone NN10. This result indicates an unconformity in the Late Miocene age (10.606–8.20 Ma).
{"title":"Nannoplankton Biostratigraphy from Banggai-Sula Basin, Central Sulawesi","authors":"Efrilia Mahdilah Nurhidayah, A. Akmaluddin, D. Barianto, Salahuddin Husein, Asep Saripudin","doi":"10.22146/jtbb.85308","DOIUrl":"https://doi.org/10.22146/jtbb.85308","url":null,"abstract":"The nannoplankton research was conducted in the MH-2 well, Banggai-Sula Basin, Central Sulawesi. Thirty-four ditch-cutting samples were utilized to observe the Minahaki, Kintom, and Biak Formations. Age determination was carried out using biostratigraphy method and standard procedure for first and last occurrence of nannoplankton biodatum and had an absolute age, widely known as a zone indicator. This study aims to determine the age and nannoplankton biozonation of each geological formation in Banggai-Sula Basin. Based on the biostratigraphic analysis, 39 species from 14 genera with abundance frequencies ranging from rare to abundant were found in the MH-2 well. In this study, new results of the age of Banggai-Sula Basin areMiddle Miocene – Early Middle Pliocene (13,706 – 3,727 Ma), and can identify into six calcareous nannoplankton zones that are more detailed than previous researchers, Discoaster signus zone (NN5), Discoaster exilis zone (NN6-NN7), Discoaster berggrenii zone (NN11), Ceratolithus acustus zone (NN12), Helicosphaera sellii zone (NN13-NN15), and the Discoaster tamalis zone (NN16). Biostratigraphic data also shows new information for the first time, the absence of three zones from zone NN8 to zone NN10. This result indicates an unconformity in the Late Miocene age (10.606–8.20 Ma). ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"1 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140396165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. P. Putra, O. D. Nurhayat, M. Sibero, R. Hermawan
The genus Pleurotus is known as a commercially important mushroom and one of the most well-known cultivated mushrooms worldwide. Of many species of Pleurotus, the phoenix mushroom (P. pulmonarius) is cultivated in many countries, including Indonesia. In Indonesia, the farmers and larger companies usually use commercial strains of phoenix mushroom which they purchased from other countries. To date, there was no prior information regarding wild occurrences of P. pulmonarius in Indonesia. During our regular mushroom hunting in Sukabumi, West Java, Indonesia, some edible wild fruiting bodies of light brown Pleurotus were collected. The current study aimed to determine the taxonomical position of our specimens based on morphological and molecular evidence. The combination of morphological and molecular analysis confirmed our specimens as P. pulnonarius. Morphologically, our specimens were distinguished by the small to medium sized fruiting bodies, pileus light brown, pinkish brown, to pale brown, flabelliform in the beginning to expanding broadly ovoid in maturity, lamellae shortly to deeply decurrent, stipe fleshy, eccentric to lateral, concolorous with lamellae, Basidiospores cylindrical to ellipsoid, basidia clavate to club shaped, basidioles are abundant, oleiferous hyphae common. The BLAST result revealed that our specimens posed a high similarity to P. pulnonarius from several countries as the top hits. The ITS phylogenetic tree placed Pleurotus FIPIA-DEP51 in the same clade of P. pulnonarius with 100% BS value. This study reports for the first time the wild occurences of P. pulmonarius in Indonesia. Future study should be done to characterize the cultures of reported mushroom which can potentially be the local strain for cultivation of P. pulmonarius industry in Indonesia.
众所周知，凤尾菇属是一种具有重要商业价值的蘑菇，也是世界上最著名的栽培蘑菇之一。在众多凤尾菇品种中，凤尾菇（P. pulmonarius）在许多国家都有栽培，包括印度尼西亚。在印度尼西亚，农民和大型公司通常使用从其他国家购买的商业凤尾菇菌株。迄今为止，还没有关于印尼野生凤尾菇的信息。我们在印尼西爪哇苏卡布米定期采菇时，采集到了一些可食用的浅棕色凤尾菇野生子实体。本研究旨在根据形态学和分子证据确定标本的分类位置。结合形态学和分子分析，确认我们的标本为 P. pulnonarius。从形态上看，我们的标本具有小到中等大小的子实体，绒毛浅褐色、粉褐色到淡褐色，从最初的扇形到成熟时扩大为宽卵形，薄片短到深下延，柄肉质，偏心到侧生，与薄片同色，基生孢子圆柱形到椭圆形，基部棍棒状到棍棒状，基部小管丰富，含油菌丝常见。BLAST 结果显示，我们的标本与来自多个国家的 P. pulnonarius 具有高度相似性，为点击率最高的标本。ITS 系统发生树将 Pleurotus FIPIA-DEP51 放在 P. pulnonarius 的同一支系中，BS 值为 100%。本研究首次报道了印尼P. pulmonarius的野生分布情况。今后的研究应该对所报道的蘑菇培养物进行特征描述，这些培养物有可能成为印尼培植猪肺菌产业的本地菌株。
{"title":"First Report on Wild Occurrences of Phoenix Mushroom (Pleurotus pulmonarius Fr. Quél.) in Indonesia","authors":"I. P. Putra, O. D. Nurhayat, M. Sibero, R. Hermawan","doi":"10.22146/jtbb.86455","DOIUrl":"https://doi.org/10.22146/jtbb.86455","url":null,"abstract":"The genus Pleurotus is known as a commercially important mushroom and one of the most well-known cultivated mushrooms worldwide. Of many species of Pleurotus, the phoenix mushroom (P. pulmonarius) is cultivated in many countries, including Indonesia. In Indonesia, the farmers and larger companies usually use commercial strains of phoenix mushroom which they purchased from other countries. To date, there was no prior information regarding wild occurrences of P. pulmonarius in Indonesia. During our regular mushroom hunting in Sukabumi, West Java, Indonesia, some edible wild fruiting bodies of light brown Pleurotus were collected. The current study aimed to determine the taxonomical position of our specimens based on morphological and molecular evidence. The combination of morphological and molecular analysis confirmed our specimens as P. pulnonarius. Morphologically, our specimens were distinguished by the small to medium sized fruiting bodies, pileus light brown, pinkish brown, to pale brown, flabelliform in the beginning to expanding broadly ovoid in maturity, lamellae shortly to deeply decurrent, stipe fleshy, eccentric to lateral, concolorous with lamellae, Basidiospores cylindrical to ellipsoid, basidia clavate to club shaped, basidioles are abundant, oleiferous hyphae common. The BLAST result revealed that our specimens posed a high similarity to P. pulnonarius from several countries as the top hits. The ITS phylogenetic tree placed Pleurotus FIPIA-DEP51 in the same clade of P. pulnonarius with 100% BS value. This study reports for the first time the wild occurences of P. pulmonarius in Indonesia. Future study should be done to characterize the cultures of reported mushroom which can potentially be the local strain for cultivation of P. pulmonarius industry in Indonesia. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"6 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140396680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fauziah Novita Putri Rifai, Mila Hanifa, Ummi Maryam Zulfin, Muthi’ Ikawati, E. Meiyanto
Pentagamavunone-1 (PGV-1), a curcumin analog, is a promising anticancer candidate for several cancers that have been proven in vitro and in vivo. However, the efficacy of PGV-1 against breast cancer is subject to improvement to achieve a more suitable application. Here we propose hesperitin, a citrus flavonoid, to increase the anticancer potency of PGV-1 in luminal breast cancer cells. We use the T47D cell as the model to investigate the effect of co-administration of PGV-1 and hesperitin on cell cycle block, apoptosis modulation, and senescence phenomena. PGV-1 and hesperitin showed strong and weak cytotoxicity with an IC50 value of 2 µM and 100 µM, respectively. The co-treatment of PGV-1 and hesperitin resulted in strong synergistic effects with combination index (CI) value of ≤ 0.2. This combination caused apoptosis in correlation with cell cycle disruption in G2/M phase at 48 h. In particular, PGV-1 and hesperitin combination increased the incidence of cellular senescence significantly higher than the single treatment. Despite its senescence potentiation, hesperitin did not induce senescence in normal cells. Taken together, hesperitin may increase the anticancer potency of PGV-1 by modulating cell cycle arrest and apoptosis via the senescence mechanism.
{"title":"Hesperitin Synergistically Promotes the Senescence Induction of Pentagamavunone-1 in Luminal Breast Cancer Cells, T47D","authors":"Fauziah Novita Putri Rifai, Mila Hanifa, Ummi Maryam Zulfin, Muthi’ Ikawati, E. Meiyanto","doi":"10.22146/jtbb.88238","DOIUrl":"https://doi.org/10.22146/jtbb.88238","url":null,"abstract":"Pentagamavunone-1 (PGV-1), a curcumin analog, is a promising anticancer candidate for several cancers that have been proven in vitro and in vivo. However, the efficacy of PGV-1 against breast cancer is subject to improvement to achieve a more suitable application. Here we propose hesperitin, a citrus flavonoid, to increase the anticancer potency of PGV-1 in luminal breast cancer cells. We use the T47D cell as the model to investigate the effect of co-administration of PGV-1 and hesperitin on cell cycle block, apoptosis modulation, and senescence phenomena. PGV-1 and hesperitin showed strong and weak cytotoxicity with an IC50 value of 2 µM and 100 µM, respectively. The co-treatment of PGV-1 and hesperitin resulted in strong synergistic effects with combination index (CI) value of ≤ 0.2. This combination caused apoptosis in correlation with cell cycle disruption in G2/M phase at 48 h. In particular, PGV-1 and hesperitin combination increased the incidence of cellular senescence significantly higher than the single treatment. Despite its senescence potentiation, hesperitin did not induce senescence in normal cells. Taken together, hesperitin may increase the anticancer potency of PGV-1 by modulating cell cycle arrest and apoptosis via the senescence mechanism. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140398024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Centella asiatica (L.) Urb. is a plant species native to Java and one of the main basic materials in traditional and modern medicine. This study is the first to report the intraspecific variation and taxonomic relationships of C. asiatica accession from natural populations in Central Java. The purpose of this study was to reveal phenotypic variations of C. asiatica populations and to asses phenetic relationships based on morphological characters. Thirty-two accessions of C. asiatica were collected from natural populations from eight mountains in Central Java. Observation on vegetative organs resulted in 25 morphological characters as a basis for assessing phenetic relationships using cluster analysis and principal component analysis. Result of cluster analysis showed that the grouping of accessions was not correlated to the localities from where the samples were collected, although there was a tendency that accessions from the same localities grouped in one cluster. The results of this study confirmed the existence of intraspecies morphological variability in C. asiatica which was not affected by geographical aspects. Results of principal component analysis indicated that the grouping of accessions was mainly determined by similarities in petiole color, stolon color, leaf margin, petiole length, stolon length, and leaf color. Given that the characters contributing to the grouping of accessions were mainly qualitative characters, the results indicated a genetic basis underlying phenotypic variations of C. asiatica accession.
积雪草（Centella asiatica (L.) Urb.）是一种原产于爪哇的植物物种，也是传统和现代医学的主要基础材料之一。本研究首次报告了中爪哇自然种群中积雪草的种内变异和分类学关系。 本研究的目的是揭示 C. asiatica 种群的表型变异，并根据形态特征评估表型关系。研究人员从爪哇中部 8 个山区的自然种群中采集了 32 个 C. asiatica 加入品系。通过对无性器官的观察，得出了 25 个形态特征，并以此为基础，采用聚类分析和主成分分析法评估表型关系。聚类分析的结果表明，尽管同一地点的样本有聚为一类的趋势，但样本的分组与样本采集地点无关。该研究结果证实，亚洲芹属植物存在种内形态变异，且不受地理因素的影响。主成分分析结果表明，插种的分组主要由叶柄颜色、匍匐茎颜色、叶缘、叶柄长度、匍匐茎长度和叶色的相似性决定。考虑到导致插种分组的特征主要是定性特征，该结果表明茜草插种的表型变异具有遗传基础。
{"title":"Intraspecific Variability and Phenetic Relationships of Centella Asiatica (L.) Urb. Accessions from Central Java Based on Morphological Characters’","authors":"A. Maruzy, R. Susandarini","doi":"10.22146/jtbb.86477","DOIUrl":"https://doi.org/10.22146/jtbb.86477","url":null,"abstract":"Centella asiatica (L.) Urb. is a plant species native to Java and one of the main basic materials in traditional and modern medicine. This study is the first to report the intraspecific variation and taxonomic relationships of C. asiatica accession from natural populations in Central Java. The purpose of this study was to reveal phenotypic variations of C. asiatica populations and to asses phenetic relationships based on morphological characters. Thirty-two accessions of C. asiatica were collected from natural populations from eight mountains in Central Java. Observation on vegetative organs resulted in 25 morphological characters as a basis for assessing phenetic relationships using cluster analysis and principal component analysis. Result of cluster analysis showed that the grouping of accessions was not correlated to the localities from where the samples were collected, although there was a tendency that accessions from the same localities grouped in one cluster. The results of this study confirmed the existence of intraspecies morphological variability in C. asiatica which was not affected by geographical aspects. Results of principal component analysis indicated that the grouping of accessions was mainly determined by similarities in petiole color, stolon color, leaf margin, petiole length, stolon length, and leaf color. Given that the characters contributing to the grouping of accessions were mainly qualitative characters, the results indicated a genetic basis underlying phenotypic variations of C. asiatica accession. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"60 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140406471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amalia Rahmawati, Irma Rohmawati, Istini Nurafifah, B. Sadewo, E. Suyono
Bioflocculation is a harvesting technique that employs flocculant agents such as bacteria and microalgae. The benefit is the absence of a chemical-added flocculant. Because bacteria need a particular medium, microalgae flocculant agents are more effective. This study used Anabaena sp. to collect fat, protein, and carbohydrates from the Glagah consortium. Three replications of those microalgae were grown in 300 ml of Bold Basal Medium culture for eight days. On the day of harvest, flocculant microalgae (Anabaena sp.) and non-flocculant microalgae (Glagah) were combined to accomplish flocculation. On the day of harvest, parameters were observed by combining Anabaena sp. with the Glagah consortium in the ratios 1: 1, 0.5: 1, and 0.25: 1. There were three times of each parameter test. Utilizing a wavelength of 750 nm, the proportion of precipitation was calculated spectrophotometrically. Bligh and Dyer were used to measure the lipids. The phenol sulfate technique was used to calculate the amount of carbohydrates. By employing the Bradford method, proteins were quantified. Biofocculation percentages and carbohydrate content were optimum on a ratio of 0.25:1. Lipid and protein content were optimum on a ratio of 1:1.
{"title":"Growth Kinetic Modelling of Efficient Anabaena sp. Bioflocculation","authors":"Amalia Rahmawati, Irma Rohmawati, Istini Nurafifah, B. Sadewo, E. Suyono","doi":"10.22146/jtbb.82196","DOIUrl":"https://doi.org/10.22146/jtbb.82196","url":null,"abstract":"Bioflocculation is a harvesting technique that employs flocculant agents such as bacteria and microalgae. The benefit is the absence of a chemical-added flocculant. Because bacteria need a particular medium, microalgae flocculant agents are more effective. This study used Anabaena sp. to collect fat, protein, and carbohydrates from the Glagah consortium. Three replications of those microalgae were grown in 300 ml of Bold Basal Medium culture for eight days. On the day of harvest, flocculant microalgae (Anabaena sp.) and non-flocculant microalgae (Glagah) were combined to accomplish flocculation. On the day of harvest, parameters were observed by combining Anabaena sp. with the Glagah consortium in the ratios 1: 1, 0.5: 1, and 0.25: 1. There were three times of each parameter test. Utilizing a wavelength of 750 nm, the proportion of precipitation was calculated spectrophotometrically. Bligh and Dyer were used to measure the lipids. The phenol sulfate technique was used to calculate the amount of carbohydrates. By employing the Bradford method, proteins were quantified. Biofocculation percentages and carbohydrate content were optimum on a ratio of 0.25:1. Lipid and protein content were optimum on a ratio of 1:1.","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"178 S439","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140428648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Sudiarta, Shah Mahapati Dinarkaya, Komang Saraswati, I. Putu, Bawa Ariyanta, Gusti Ngurah, Alit Susanta Wirya, Dwi Sugiarta, Dewa Gede, Wiryangga Selangga, Wayan Diksa Gargita, Putu Perdana, Kusuma Wiguna, K. A. Yuliadhi, Putu Shinta Devi
Cassava Lace Bugs (CLB) are native pest of cassava (Manihot esculenta Crantz, Euphorbiaceae) to the Neotropical Region, mainly in Brazil. On the other hand, East Java was the first region in Indonesia to record the presence of CLB in 2021, however, it has not been reported in other regions in Indonesia. Therefore, the very importance to recognise the occurrence of CLB in other regions in Indonesia. Based on this, the research has been carried out starting with a field survey, observing behaviour of insect in the field and identify morphologically in the laboratory. The survey results show that the infestation of CLB has been found in lowland, medium, and highland areas in Bali. Symptoms of infestation on the upper leaf surface are small yellow spots with brownish variations. Based on the identification key, CLB from Bali Indonesia, shows a characteristic of a head with a pair of frontal spines. Based on this evidence, the CLB insect can be identified as Vatiga illudens (Hemiptera: Heteroptera: Tingidae). It is the first report of novel distribution areas for V. illudens in Bali, Indonesia. The results of this research are important because V. illudens is one of the main pests of cassava.
{"title":"Occurrence of Cassava Lace Bug Vatiga illudens (Drake, 1922) (Hemiptera: Heteroptera: Tingidae) in Bali, Indonesia","authors":"I. Sudiarta, Shah Mahapati Dinarkaya, Komang Saraswati, I. Putu, Bawa Ariyanta, Gusti Ngurah, Alit Susanta Wirya, Dwi Sugiarta, Dewa Gede, Wiryangga Selangga, Wayan Diksa Gargita, Putu Perdana, Kusuma Wiguna, K. A. Yuliadhi, Putu Shinta Devi","doi":"10.22146/jtbb.87438","DOIUrl":"https://doi.org/10.22146/jtbb.87438","url":null,"abstract":"Cassava Lace Bugs (CLB) are native pest of cassava (Manihot esculenta Crantz, Euphorbiaceae) to the Neotropical Region, mainly in Brazil. On the other hand, East Java was the first region in Indonesia to record the presence of CLB in 2021, however, it has not been reported in other regions in Indonesia. Therefore, the very importance to recognise the occurrence of CLB in other regions in Indonesia. Based on this, the research has been carried out starting with a field survey, observing behaviour of insect in the field and identify morphologically in the laboratory. The survey results show that the infestation of CLB has been found in lowland, medium, and highland areas in Bali. Symptoms of infestation on the upper leaf surface are small yellow spots with brownish variations. Based on the identification key, CLB from Bali Indonesia, shows a characteristic of a head with a pair of frontal spines. Based on this evidence, the CLB insect can be identified as Vatiga illudens (Hemiptera: Heteroptera: Tingidae). It is the first report of novel distribution areas for V. illudens in Bali, Indonesia. The results of this research are important because V. illudens is one of the main pests of cassava. ","PeriodicalId":52402,"journal":{"name":"Journal of Tropical Biodiversity and Biotechnology","volume":"22 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140437909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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