María de la Paz Moliné, M. Magdalena Vázquez, Paloma Moran Giardini, Enzo Domínguez, Natalia J. Fernández, Natalia Damiani, Silvina Quintana, Liesel B. Gende
{"title":"酿酒酵母菌对蜜蜂幼虫免疫和抗氧化基因表达的刺激作用","authors":"María de la Paz Moliné, M. Magdalena Vázquez, Paloma Moran Giardini, Enzo Domínguez, Natalia J. Fernández, Natalia Damiani, Silvina Quintana, Liesel B. Gende","doi":"10.1111/jen.13235","DOIUrl":null,"url":null,"abstract":"<p>The high overwinter mortalities in the honey bee population of <i>Apis mellifera</i> have been linked to immunocompromised individuals experiencing nutritional limitations. Larval nutrition plays a crucial role in determining the successful development of adults, making them more susceptible to bacterial diseases when undernourished. One of the main intracellular signalling pathways in the larval immune system against bacterial diseases is the Toll receptor signalling pathway, which activates antimicrobial peptides like defensin. Inadequate nutrition also disrupts the redox balance on <i>A. mellifera</i> individuals, with superoxide dismutase and catalase being the key components of their antioxidant system. Vitellogenin is associated with the mobilization of nutrients in early <i>A. mellifera</i> bee stages. Considering that <i>Saccharomyces cerevisiae</i> is the second most significant by-product of the brewing industry, it may serve as a valuable raw material. We investigated the impact of feeding larvae with live <i>S. cerevisiae</i> cells on the relative expression of their main immune and antioxidant-related genes. To assess gene expression, we conducted RT-qPCR, using RNA extracted from ten larvae per treatment on the seventh day when the feeding trial concluded. Importantly, the presence of <i>S. cerevisiae</i> did not affect larval survival compared to the control group. However, the relative expression of immune-related gene <i>toll18W</i> and antioxidant-related gene <i>sod</i> in larvae fed with live <i>S. cerevisiae</i> cells was significantly higher than in larvae that were not fed with them. This suggests that the inclusion of <i>S. cerevisiae</i> in their diet may provide larvae with a nutritional advantage. Additionally, the vitellogenin (<i>vg</i>) gene expression increased in treated larvae compared to the control group, potentially facilitating their access to nutrients and, consequently, enhancing their ability to cope with nutritional stress.</p>","PeriodicalId":14987,"journal":{"name":"Journal of Applied Entomology","volume":"148 4","pages":"382-390"},"PeriodicalIF":1.7000,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immune and antioxidant gene expression stimulation of Apis mellifera (Hymenoptera: Apidae) larvae by Saccharomyces cerevisiae from the brewering industry\",\"authors\":\"María de la Paz Moliné, M. Magdalena Vázquez, Paloma Moran Giardini, Enzo Domínguez, Natalia J. Fernández, Natalia Damiani, Silvina Quintana, Liesel B. Gende\",\"doi\":\"10.1111/jen.13235\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The high overwinter mortalities in the honey bee population of <i>Apis mellifera</i> have been linked to immunocompromised individuals experiencing nutritional limitations. Larval nutrition plays a crucial role in determining the successful development of adults, making them more susceptible to bacterial diseases when undernourished. One of the main intracellular signalling pathways in the larval immune system against bacterial diseases is the Toll receptor signalling pathway, which activates antimicrobial peptides like defensin. Inadequate nutrition also disrupts the redox balance on <i>A. mellifera</i> individuals, with superoxide dismutase and catalase being the key components of their antioxidant system. Vitellogenin is associated with the mobilization of nutrients in early <i>A. mellifera</i> bee stages. Considering that <i>Saccharomyces cerevisiae</i> is the second most significant by-product of the brewing industry, it may serve as a valuable raw material. We investigated the impact of feeding larvae with live <i>S. cerevisiae</i> cells on the relative expression of their main immune and antioxidant-related genes. To assess gene expression, we conducted RT-qPCR, using RNA extracted from ten larvae per treatment on the seventh day when the feeding trial concluded. Importantly, the presence of <i>S. cerevisiae</i> did not affect larval survival compared to the control group. However, the relative expression of immune-related gene <i>toll18W</i> and antioxidant-related gene <i>sod</i> in larvae fed with live <i>S. cerevisiae</i> cells was significantly higher than in larvae that were not fed with them. This suggests that the inclusion of <i>S. cerevisiae</i> in their diet may provide larvae with a nutritional advantage. Additionally, the vitellogenin (<i>vg</i>) gene expression increased in treated larvae compared to the control group, potentially facilitating their access to nutrients and, consequently, enhancing their ability to cope with nutritional stress.</p>\",\"PeriodicalId\":14987,\"journal\":{\"name\":\"Journal of Applied Entomology\",\"volume\":\"148 4\",\"pages\":\"382-390\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-01-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Entomology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jen.13235\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Entomology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jen.13235","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Immune and antioxidant gene expression stimulation of Apis mellifera (Hymenoptera: Apidae) larvae by Saccharomyces cerevisiae from the brewering industry
The high overwinter mortalities in the honey bee population of Apis mellifera have been linked to immunocompromised individuals experiencing nutritional limitations. Larval nutrition plays a crucial role in determining the successful development of adults, making them more susceptible to bacterial diseases when undernourished. One of the main intracellular signalling pathways in the larval immune system against bacterial diseases is the Toll receptor signalling pathway, which activates antimicrobial peptides like defensin. Inadequate nutrition also disrupts the redox balance on A. mellifera individuals, with superoxide dismutase and catalase being the key components of their antioxidant system. Vitellogenin is associated with the mobilization of nutrients in early A. mellifera bee stages. Considering that Saccharomyces cerevisiae is the second most significant by-product of the brewing industry, it may serve as a valuable raw material. We investigated the impact of feeding larvae with live S. cerevisiae cells on the relative expression of their main immune and antioxidant-related genes. To assess gene expression, we conducted RT-qPCR, using RNA extracted from ten larvae per treatment on the seventh day when the feeding trial concluded. Importantly, the presence of S. cerevisiae did not affect larval survival compared to the control group. However, the relative expression of immune-related gene toll18W and antioxidant-related gene sod in larvae fed with live S. cerevisiae cells was significantly higher than in larvae that were not fed with them. This suggests that the inclusion of S. cerevisiae in their diet may provide larvae with a nutritional advantage. Additionally, the vitellogenin (vg) gene expression increased in treated larvae compared to the control group, potentially facilitating their access to nutrients and, consequently, enhancing their ability to cope with nutritional stress.
期刊介绍:
The Journal of Applied Entomology publishes original articles on current research in applied entomology, including mites and spiders in terrestrial ecosystems.
Submit your next manuscript for rapid publication: the average time is currently 6 months from submission to publication. With Journal of Applied Entomology''s dynamic article-by-article publication process, Early View, fully peer-reviewed and type-set articles are published online as soon as they complete, without waiting for full issue compilation.