酿酒酵母菌对蜜蜂幼虫免疫和抗氧化基因表达的刺激作用

IF 1.7 3区 农林科学 Q2 ENTOMOLOGY Journal of Applied Entomology Pub Date : 2024-01-30 DOI:10.1111/jen.13235
María de la Paz Moliné, M. Magdalena Vázquez, Paloma Moran Giardini, Enzo Domínguez, Natalia J. Fernández, Natalia Damiani, Silvina Quintana, Liesel B. Gende
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引用次数: 0

摘要

蜜蜂越冬死亡率高与免疫力低下的个体营养不良有关。幼虫的营养对成虫的成功发育起着至关重要的作用,营养不良的幼虫更容易感染细菌性疾病。幼虫免疫系统抵抗细菌疾病的主要细胞内信号途径之一是 Toll 受体信号途径,它能激活防御素等抗菌肽。营养不足也会破坏 A. mellifera 个体的氧化还原平衡,超氧化物歧化酶和过氧化氢酶是其抗氧化系统的关键组成部分。卵黄素与蜜蜂早期阶段的营养调动有关。考虑到酿酒酵母是酿酒业的第二大副产品,它可以作为一种有价值的原料。我们研究了用活的酿酒酵母细胞喂养幼虫对其主要免疫和抗氧化相关基因相对表达的影响。为了评估基因表达,我们在喂养试验结束后的第七天,使用从每种处理的 10 只幼虫中提取的 RNA 进行了 RT-qPCR 分析。重要的是,与对照组相比,麦角菌的存在并未影响幼虫的存活率。然而,在喂食了麦角菌活细胞的幼虫体内,免疫相关基因 toll18W 和抗氧化相关基因 sod 的相对表达量明显高于未喂食麦角菌活细胞的幼虫。这表明,在幼虫的食物中添加麦角菌可为幼虫提供营养优势。此外,与对照组相比,处理组幼虫的卵黄素(vg)基因表达量增加,这可能有助于它们获得营养,从而提高它们应对营养压力的能力。
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Immune and antioxidant gene expression stimulation of Apis mellifera (Hymenoptera: Apidae) larvae by Saccharomyces cerevisiae from the brewering industry

The high overwinter mortalities in the honey bee population of Apis mellifera have been linked to immunocompromised individuals experiencing nutritional limitations. Larval nutrition plays a crucial role in determining the successful development of adults, making them more susceptible to bacterial diseases when undernourished. One of the main intracellular signalling pathways in the larval immune system against bacterial diseases is the Toll receptor signalling pathway, which activates antimicrobial peptides like defensin. Inadequate nutrition also disrupts the redox balance on A. mellifera individuals, with superoxide dismutase and catalase being the key components of their antioxidant system. Vitellogenin is associated with the mobilization of nutrients in early A. mellifera bee stages. Considering that Saccharomyces cerevisiae is the second most significant by-product of the brewing industry, it may serve as a valuable raw material. We investigated the impact of feeding larvae with live S. cerevisiae cells on the relative expression of their main immune and antioxidant-related genes. To assess gene expression, we conducted RT-qPCR, using RNA extracted from ten larvae per treatment on the seventh day when the feeding trial concluded. Importantly, the presence of S. cerevisiae did not affect larval survival compared to the control group. However, the relative expression of immune-related gene toll18W and antioxidant-related gene sod in larvae fed with live S. cerevisiae cells was significantly higher than in larvae that were not fed with them. This suggests that the inclusion of S. cerevisiae in their diet may provide larvae with a nutritional advantage. Additionally, the vitellogenin (vg) gene expression increased in treated larvae compared to the control group, potentially facilitating their access to nutrients and, consequently, enhancing their ability to cope with nutritional stress.

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来源期刊
CiteScore
3.40
自引率
5.30%
发文量
132
审稿时长
6 months
期刊介绍: The Journal of Applied Entomology publishes original articles on current research in applied entomology, including mites and spiders in terrestrial ecosystems. Submit your next manuscript for rapid publication: the average time is currently 6 months from submission to publication. With Journal of Applied Entomology''s dynamic article-by-article publication process, Early View, fully peer-reviewed and type-set articles are published online as soon as they complete, without waiting for full issue compilation.
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