Chemical components and antimicrobial activity of essential oil from leaf of Aristolochia balansae Franch.

Aristolochia balansae Franch. belonging to Aristolochiasubgenus Siphisia (Aristolochiaceae) is rarely distributed in Northern Vietnam. This study is the first time to determine the chemical composition and antimicrobial activity from essential oils of leaves collected in Ba Thuoc district, Thanh Hoa province in November 2021. The essential oils from the leaves of A. balansae were obtained by hydrodistillation using Clevenger-type apparatus and analysed by gas chromatography coupled with mass spectrometry. The yield of the essential oil was 0.15% of fresh weight, the essential oil is light yellow, lighter than water. Sixty-three components were identified, which present 78.58% of the total composition of the leaf’s essential oil. The main components were sesquiterpenes and oxygenated sesquiterpenes. The constituents occurring in higher amounts in the essential oil were γ-Eudesmol (6.98% of the total composition of the leaf’s essential oil), α-Humulene (6.22%), β-Selinene (4.5%), Eudesma-4(14),7(11)-diene (3.53%), α-trans-Bergamotene (3.27%), and Limonene (3.02%). This essential oil showed weak antimicrobial activities to most of the tested microbial strains in a concentration of 10%.