从土耳其甜菜种植区分离和鉴定潜在的生物控制剂 Podospora flexuosa(同义。

Meltem Avan, Rıza Kaya
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摘要

甜菜(Beta vulgaris L.)在土耳其科尼亚省广泛种植,该省的甜菜产量约占土耳其甜菜产量的三分之一。2015-2017 年,从该地区甜菜田中出现严重根腐病和干燥症状的植株中分离出了一种新的内生真菌分离物,同时从叶片中分离出的还有属于不同真菌种类的土壤和叶面病原物。该真菌分离物呈暗黄色、浅橄榄色至浅褐色,快速生长的菌落状、柔韧的分生孢子梗和有隔的分枝菌丝。具有代表性的真菌分离物不会对寄主植物叶片造成病害症状。利用 ITS 基因区的特异引物对该真菌分离物进行了分子鉴定,根据形态和分子特征确定其为柔毛癣菌(Podospora flexuosa)(Cladorrhinum flexuosum 的异名)。据报道,P. flexuosa 是一种生活在土壤和植物材料中的生物防治剂,因此我们认为本研究中获得的真菌分离物可作为一种潜在的生物防治剂,用于未来植物病害的生物防治。据我们所知,这种真菌物种在土尔其尚属首次报道。
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Türkiye’de şeker pancarı ekim alanlarından tespit edilen potansiyel biyokontrol ajanı Podospora flexuosa (syn. Cladorrhinum flexuosum)’nın izolasyonu ve tanımlanması
Sugar beet (Beta vulgaris L.) is widely cultivated in Konya province of Türkiye and about one third of Türkiye’s sugar beet production is obtained from this province. As a result of the isolations made from plants showing severe root rot and desiccation symptoms in sugar beet fields in the region in 2015-2017, a new endophytic fungal isolate was obtained from leaves along with soil and foliar disease agents belonging to different fungal species. The fungal isolate has dull yellow, light olive to light brown, fast growing colony-like, flexible conidiophores and septate branched hyphae. The representative fungal isolate did not cause disease symptoms on host plant leaves. Molecular identification of the fungal isolate was carried out using primers specific to the ITS gene region, and it was identified as Podospora flexuosa (syn. Cladorrhinum flexuosum) based on morphological and molecular characteristics. Since P. flexuosa is reported to be a biological control agent living as a saprophyte in soil and plant materials, it is thought that the fungal isolate obtained in our study can be used as a potential biocontrol agent in the biological control of plant disease agents in the future. To the best of our knowledge, this fungal species is reported for the first time for Türkiye.
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