Riptortus pedestris (Hemiptera: Alydidae) 体细胞 piRNA 和 PIWI 基因的鉴定与表达模式

IF 1.5 4区 农林科学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Archives of Insect Biochemistry and Physiology Pub Date : 2024-04-09 DOI:10.1002/arch.22107
Yipeng Ren, Wenhao Dong, Wenjun Bu, Huaijun Xue
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引用次数: 0

摘要

基于 RNA 干扰(RNAi)的基因沉默是一种可行且可持续的技术,可通过双链 RNA(包括小干扰 RNA、microRNA 和 Piwi-interacting RNA (piRNA))的参与来治理半翅目害虫,有助于减少化学杀虫剂的使用。然而,关于大豆(Glycine max)的主要害虫 Riptortus pedestris 的体细胞 piRNA 及其生物发生基因,目前只有少量数据。在这项研究中,发现并鉴定了大豆蓟马中的两个 PIWI 基因家族成员,其中包括具有保守蛋白结构域的 Argonaute3(RpAgo3)和 Aubergine(RpAub)基因,并通过系统发育分析验证了它们的聚类。此外,根据之前的转录组测序结果,它们在梗蛙全身各发育阶段均有广泛表达,而在不同饲养条件下,它们在梗蛙内脏中的表达水平较低。此外,根据我们之前的全转录组测序结果,通过将丰富的纯净读数映射到基质基因组上,共筛选出 45 998 个 piRNA,这些 piRNA 的第一个核苷酸(nt)位置存在尿苷偏倚,长度为 26-32 nt。最后,我们的数据显示,通过皮尔逊相关分析,肠道细菌的变化与五个对比组中不同表达的 piRNAs 显著正相关或负相关。总之,这些发现为应用基于 RNAi 的生物农药广谱防治半翅目害虫铺平了新的道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Identification and expression patterns of somatic piRNAs and PIWI genes in Riptortus pedestris (Hemiptera: Alydidae)

RNA interference (RNAi)-based gene silencing is a feasible and sustainable technology for the management of hemipteran pests by double-stranded RNA involvement, including small-interfering RNA, microRNA, and Piwi-interacting RNA (piRNA) pathways, that may help to decrease the usage of chemical insecticides. However, only a few data are available on the somatic piRNAs and their biogenesis genes in Riptortus pedestris, which serves as a significant pest of soybean (Glycine max). In this study, two family members of the PIWI gene were identified and characterized in R. pedestris, containing Argonaute3 (RpAgo3) and Aubergine (RpAub) genes with conserved protein domains, and their clusters were validated by phylogenetic analysis. In addition, they were widely expressed in all developmental stages of the whole body of R. pedestris and had lower expression levels in R. pedestris guts under different rearing conditions based on previous transcriptome sequencing. Furthermore, abundant clean reads were filtered to a total number of 45,998 piRNAs with uridine bias at the first nucleotide (nt) position and 26–32 nt in length by mapping onto the reference genome of R. pedestris according to our previous whole-transcriptome sequencing. Finally, our data revealed that gut bacterial changes were significantly positively or negatively associated with differentially expressed piRNAs among the five comparison groups with Pearson correlation analysis. In conclusion, these findings paved new avenues for the application of RNAi-based biopesticides for broad-spectrum hemipteran pest control.

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来源期刊
CiteScore
4.30
自引率
4.50%
发文量
115
审稿时长
12 months
期刊介绍: Archives of Insect Biochemistry and Physiology is an international journal that publishes articles in English that are of interest to insect biochemists and physiologists. Generally these articles will be in, or related to, one of the following subject areas: Behavior, Bioinformatics, Carbohydrates, Cell Line Development, Cell Signalling, Development, Drug Discovery, Endocrinology, Enzymes, Lipids, Molecular Biology, Neurobiology, Nucleic Acids, Nutrition, Peptides, Pharmacology, Pollinators, Proteins, Toxicology. Archives will publish only original articles. Articles that are confirmatory in nature or deal with analytical methods previously described will not be accepted.
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