利用代谢工程大肠杆菌微生物生产含硫氨基酸

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology advances Pub Date : 2024-04-07 DOI:10.1016/j.biotechadv.2024.108353
Lijuan Wang , Yingying Guo , Yizhou Shen , Kun Yang , Xue Cai , Bo Zhang , Zhiqiang Liu , Yuguo Zheng
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引用次数: 0

摘要

L-Cysteine 和 L-methionine 是 20 种典型氨基酸中仅有的两种含硫氨基酸,具有独特的特性,在工业上有着广泛的应用。利用不同的生物发酵生产 L-半胱氨酸和 L-蛋氨酸正受到越来越多的关注,其中被广泛研究的是首选菌株。这种偏好是由于它能在具有成本效益的培养基中快速生长,对工业过程具有很强的适应性,新陈代谢特征明显,而且有用于基因工程的分子工具。本综述重点介绍了......中生产这些含硫氨基酸所涉及的遗传和分子机制。此外,我们还系统地总结了为提高其产量而采用的代谢工程策略,包括确定新的目标、调节代谢通量、改造转运系统、动态调节策略和优化发酵条件。本综述中讨论的策略和设计原则有可能促进直接发酵含硫氨基酸的菌种和工艺工程的发展。
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Microbial production of sulfur-containing amino acids using metabolically engineered Escherichia coli

L-Cysteine and L-methionine, as the only two sulfur-containing amino acids among the canonical 20 amino acids, possess distinct characteristics and find wide-ranging industrial applications. The use of different organisms for fermentative production of L-cysteine and L-methionine is gaining increasing attention, with Escherichia coli being extensively studied as the preferred strain. This preference is due to its ability to grow rapidly in cost-effective media, its robustness for industrial processes, the well-characterized metabolism, and the availability of molecular tools for genetic engineering. This review focuses on the genetic and molecular mechanisms involved in the production of these sulfur-containing amino acids in E. coli. Additionally, we systematically summarize the metabolic engineering strategies employed to enhance their production, including the identification of new targets, modulation of metabolic fluxes, modification of transport systems, dynamic regulation strategies, and optimization of fermentation conditions. The strategies and design principles discussed in this review hold the potential to facilitate the development of strain and process engineering for direct fermentation of sulfur-containing amino acids.

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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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