响应面优化深共晶溶剂提取竹笋蛋白质的方法

Siyi Wang, Qifan Liu, Chang Xu, Yuan Gao, Xuejun Yu
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引用次数: 0

摘要

为了提高竹笋蛋白质的提取率,采用响应面法对竹笋蛋白质的提取工艺进行了优化。在总共八种深共晶溶剂中,氯化胆碱和脯氨酸被用作氢键受体,而葡萄糖、蔗糖、甘油、乙二醇、乙酰丙酸、苹果酸和乳酸被用作氢键供体。这项研究考察了使用深共晶溶剂从竹笋中提取蛋白质的过程。通过单因素实验确定了深共晶溶剂的最佳成分,以及理想的料液比梯度、含水量、萃取时间和水浴温度。采用箱-贝肯设计法和响应面法研究了各种变量对竹笋蛋白质提取率的影响和相互作用。水浴温度和提取时间都对竹笋蛋白质提取率有显著影响。值得注意的是,提取时间与水浴温度之间的交互作用对竹笋蛋白质提取率的影响最大。竹笋蛋白质提取率相对于各因素实际值的二次响应面回归方程为蛋白质提取率 = -193.3275 + 3.8395 A - 18.7075 B + 6.3285 C + 0.2125 AB - 0.0089 AC + 0.024 BC - 0.02885 A2 + 0.685 B2 - 0.06455 C2。最后,本研究预测了氯化胆碱-蔗糖(摩尔比为 1:2)、料液比为 1:50、含水量为 74%、提取时间为 4 h、水浴温度为 44 ℃的竹笋最佳提取参数。在最佳条件下,蛋白质提取率为 59.39±0.45%,与响应面拟合方程的预测值一致。这表明优化回归方程可能具有实际意义。与传统的氢氧化钠(NaOH)溶液萃取相比,深层共晶溶剂萃取竹笋更环保、无毒、无害。主要信息:为提高竹笋蛋白质提取率,采用响应面法对竹笋蛋白质提取工艺进行了优化。
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Response surface optimization for deep eutectic solvents extraction of bamboo shoots proteins

To enhance the protein extraction rate of bamboo shoots, the Response Surface Methodology was utilized to optimize the protein extraction process of Chimonobambusa purpurea Hsueh f. & T.P.Yi (syn. Chimonobambusa neopurpurea T.P.Yi) bamboo shoots. Choline chloride and proline were utilized as hydrogen bond acceptors in a total of eight deep eutectic solvents, whereas glucose, sucrose, glycerol, ethylene glycol, acetylpropionic acid, malic acid, and lactic acid were used as hydrogen bond donors. This work examined the protein extraction from bamboo shoots using deep eutectic solvents. Single-factor experiments were conducted to determine the optimal composition of the deep eutectic solvents, along with the ideal gradients of material-liquid ratio gradients, water content, extraction time, and water bath temperature. The impact and interplay of various variables on the protein extraction rate of bamboo shoots were examined using the Box-Behnken Design and Response Surface Methodology. Both the water bath temperature and the extraction time significantly influenced the protein extraction rate of bamboo shoots. Notably, the interaction between extraction time and water bath temperature had the most substantial effect on the protein extraction rate of bamboo shoots. The quadratic response surface regression equation for the protein extraction rate of bamboo shoots relative to the actual values of each factor was obtained as follows: Protein extraction rate = −193.3275 + 3.8395 A - 18.7075 B + 6.3285 C + 0.2125 AB - 0.0089 AC + 0.024 BC - 0.02885 A2 + 0.685 B2 - 0.06455 C2. Finally, this work predicted optimum extraction parameters for bamboo shoots with choline chloride-sucrose (molar ratio 1:2), a material-liquid ratio of 1:50, a water content of 74 %, an extraction time of 4 h, and a water bath temperature of 44 ℃. Under the optimal conditions, the protein extraction yield was 59.39±0.45 %, which aligned with the predicted value from the response surface fitting equation. This indicated that the optimised regression equation may have practical significance. Compared with conventional extraction using a sodium hydroxide (NaOH) solution, deep eutectic solvents extraction of bamboo shoots is more environmentally friendly, non-toxic and harmless. It provides a theoretical and experimental reference for industrial extraction and offers a reference for deep eutectic solvent development in bamboo shoot resource utilization.

Key message:

To enhance the protein extraction rate of bamboo shoots, the Response Surface Methodology was utilized to optimize the protein extraction process of bamboo shoots.

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