日本鳗鲡性腺性别分化过程中脑垂体中cyp19a1、sf-1、esrs和gths的表达概况

IF 2.1 3区 医学 Q3 ENDOCRINOLOGY & METABOLISM General and comparative endocrinology Pub Date : 2024-04-04 DOI:10.1016/j.ygcen.2024.114512
Shan-Ru Jeng , Guan-Chung Wu , Wen-Shiun Yueh , Pei-hua Liu , Shu-Fen Kuo , Sylvie Dufour , Ching-Fong Chang
{"title":"日本鳗鲡性腺性别分化过程中脑垂体中cyp19a1、sf-1、esrs和gths的表达概况","authors":"Shan-Ru Jeng ,&nbsp;Guan-Chung Wu ,&nbsp;Wen-Shiun Yueh ,&nbsp;Pei-hua Liu ,&nbsp;Shu-Fen Kuo ,&nbsp;Sylvie Dufour ,&nbsp;Ching-Fong Chang","doi":"10.1016/j.ygcen.2024.114512","DOIUrl":null,"url":null,"abstract":"<div><p>Eels are gonochoristic species whose gonadal differentiation initiates at the yellow eel stage and is influenced by environmental factors. We revealed some sex-related genes were sex dimorphically expressed in gonads during gonadal sex differentiation of Japanese eel (<em>Anguilla japonica</em>); however, the expression of sex-related genes in the brain-pituitary during gonadal sex differentiation in eels is still unclear. This study aimed to investigate the sex-related gene expressions in the brain-pituitary and tried to clarify their roles in the brain and gonads during gonadal sex differentiation. Based on our previous histological study, the control eels developed as males, and estradiol-17β (E2) was used for feminization. Our results showed that during testicular differentiation, the brain <em>cyp19a1</em> transcripts and aromatase proteins were increased significantly; moreover, the <em>cyp19a1</em>, <em>sf-1</em>, <em>foxl2s</em>, and <em>esrs</em> (except <em>gperb</em>) transcripts in the midbrain/pituitary also were increased significantly. Forebrain <em>gnrh1</em> transcripts increased slightly during gonadal differentiation of both sexes, but the <em>gnrhr1b</em> and <em>gnrhr2</em> transcripts in the midbrain/pituitary were stable during gonadal differentiation. The expression levels of <em>gths</em> and <em>gh</em> in the midbrain/pituitary were significantly increased during testicular differentiation and were much higher in males than in E2-feminized females. These results implied that endogenous estrogens might play essential roles in the brain/pituitary during testicular differentiation, <em>sf-1</em>, <em>foxl2s</em>, and <em>esrs</em> may have roles in <em>cyp19a1</em> regulation in the midbrain/pituitary of Japanese eels. For the GnRH-GTH axis, <em>gths</em>, especially <em>fshb</em>, may be regulated by <em>esrs</em> and involved in regulating testicular differentiation and development in Japanese eels.</p></div>","PeriodicalId":12582,"journal":{"name":"General and comparative endocrinology","volume":"353 ","pages":"Article 114512"},"PeriodicalIF":2.1000,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The expression profiles of cyp19a1, sf-1, esrs and gths in the brain-pituitary during gonadal sex differentiation in juvenile Japanese eels\",\"authors\":\"Shan-Ru Jeng ,&nbsp;Guan-Chung Wu ,&nbsp;Wen-Shiun Yueh ,&nbsp;Pei-hua Liu ,&nbsp;Shu-Fen Kuo ,&nbsp;Sylvie Dufour ,&nbsp;Ching-Fong Chang\",\"doi\":\"10.1016/j.ygcen.2024.114512\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Eels are gonochoristic species whose gonadal differentiation initiates at the yellow eel stage and is influenced by environmental factors. We revealed some sex-related genes were sex dimorphically expressed in gonads during gonadal sex differentiation of Japanese eel (<em>Anguilla japonica</em>); however, the expression of sex-related genes in the brain-pituitary during gonadal sex differentiation in eels is still unclear. This study aimed to investigate the sex-related gene expressions in the brain-pituitary and tried to clarify their roles in the brain and gonads during gonadal sex differentiation. Based on our previous histological study, the control eels developed as males, and estradiol-17β (E2) was used for feminization. Our results showed that during testicular differentiation, the brain <em>cyp19a1</em> transcripts and aromatase proteins were increased significantly; moreover, the <em>cyp19a1</em>, <em>sf-1</em>, <em>foxl2s</em>, and <em>esrs</em> (except <em>gperb</em>) transcripts in the midbrain/pituitary also were increased significantly. Forebrain <em>gnrh1</em> transcripts increased slightly during gonadal differentiation of both sexes, but the <em>gnrhr1b</em> and <em>gnrhr2</em> transcripts in the midbrain/pituitary were stable during gonadal differentiation. The expression levels of <em>gths</em> and <em>gh</em> in the midbrain/pituitary were significantly increased during testicular differentiation and were much higher in males than in E2-feminized females. These results implied that endogenous estrogens might play essential roles in the brain/pituitary during testicular differentiation, <em>sf-1</em>, <em>foxl2s</em>, and <em>esrs</em> may have roles in <em>cyp19a1</em> regulation in the midbrain/pituitary of Japanese eels. For the GnRH-GTH axis, <em>gths</em>, especially <em>fshb</em>, may be regulated by <em>esrs</em> and involved in regulating testicular differentiation and development in Japanese eels.</p></div>\",\"PeriodicalId\":12582,\"journal\":{\"name\":\"General and comparative endocrinology\",\"volume\":\"353 \",\"pages\":\"Article 114512\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-04-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"General and comparative endocrinology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0016648024000728\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"General and comparative endocrinology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0016648024000728","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0

摘要

鳗鱼是性染色体物种,其性腺分化始于黄鳝阶段,并受环境因素的影响。我们发现,在日本鳗鲡性腺性别分化过程中,一些与性别相关的基因在性腺中呈性别二形表达;然而,在鳗鲡性腺性别分化过程中,与性别相关的基因在脑垂体中的表达仍不清楚。本研究旨在调查脑垂体中性别相关基因的表达,并试图阐明它们在性腺性别分化过程中在大脑和性腺中的作用。根据我们之前的组织学研究,对照组鳗鱼发育为雄性,并使用雌二醇-17β(E2)进行雌性化。结果表明,在睾丸分化过程中,脑cyp19a1转录本和芳香化酶蛋白显著增加;此外,中脑/垂体的cyp19a1、sf-1、foxl2s和esrs(除gperb外)转录本也显著增加。在雌雄性腺分化过程中,前脑 gnrh1 转录本略有增加,但中脑/垂体的 gnrhr1b 和 gnrhr2 转录本在性腺分化过程中保持稳定。在睾丸分化过程中,中脑/垂体中ths和gh的表达水平显著升高,雄性远高于E2雌化的雌性。这些结果表明,在睾丸分化过程中,内源性雌激素可能在脑/垂体中发挥重要作用,sf-1、foxl2s和esrs可能在日本鳗鲡中脑/垂体的cyp19a1调控中发挥作用。在GnRH-GTH轴方面,gths,尤其是fshb可能受esrs调控,并参与调控日本鳗鲡的睾丸分化和发育。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The expression profiles of cyp19a1, sf-1, esrs and gths in the brain-pituitary during gonadal sex differentiation in juvenile Japanese eels

Eels are gonochoristic species whose gonadal differentiation initiates at the yellow eel stage and is influenced by environmental factors. We revealed some sex-related genes were sex dimorphically expressed in gonads during gonadal sex differentiation of Japanese eel (Anguilla japonica); however, the expression of sex-related genes in the brain-pituitary during gonadal sex differentiation in eels is still unclear. This study aimed to investigate the sex-related gene expressions in the brain-pituitary and tried to clarify their roles in the brain and gonads during gonadal sex differentiation. Based on our previous histological study, the control eels developed as males, and estradiol-17β (E2) was used for feminization. Our results showed that during testicular differentiation, the brain cyp19a1 transcripts and aromatase proteins were increased significantly; moreover, the cyp19a1, sf-1, foxl2s, and esrs (except gperb) transcripts in the midbrain/pituitary also were increased significantly. Forebrain gnrh1 transcripts increased slightly during gonadal differentiation of both sexes, but the gnrhr1b and gnrhr2 transcripts in the midbrain/pituitary were stable during gonadal differentiation. The expression levels of gths and gh in the midbrain/pituitary were significantly increased during testicular differentiation and were much higher in males than in E2-feminized females. These results implied that endogenous estrogens might play essential roles in the brain/pituitary during testicular differentiation, sf-1, foxl2s, and esrs may have roles in cyp19a1 regulation in the midbrain/pituitary of Japanese eels. For the GnRH-GTH axis, gths, especially fshb, may be regulated by esrs and involved in regulating testicular differentiation and development in Japanese eels.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
General and comparative endocrinology
General and comparative endocrinology 医学-内分泌学与代谢
CiteScore
5.60
自引率
7.40%
发文量
120
审稿时长
2 months
期刊介绍: General and Comparative Endocrinology publishes articles concerned with the many complexities of vertebrate and invertebrate endocrine systems at the sub-molecular, molecular, cellular and organismal levels of analysis.
期刊最新文献
Complementary and additive functions of TRα and TRβ during intestinal remodeling as revealed by ChIP-Seq analysis on wild type and TR knockout animals Zebra finches (Taeniopygia castanotis) display varying degrees of stress resilience and recovery in response to constant light. Estradiol affects the expression of essential molecular factors involved in luteinizing hormone secretion in the plains vizcacha Stress Axis: Molecular and Cellular Regulation of the HPI/HPA Axis. Spatial and quantitative gene expression analysis of SREB receptors in the gonads of green-spotted pufferfish (Dichotomyctere nigroviridis)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1