印度镰刀菌的遗传多样性和种群结构及其与鸽子豆枯萎病发病率的相关性

IF 3.5 4区 生物学 Q2 MICROBIOLOGY Journal of Basic Microbiology Pub Date : 2024-04-15 DOI:10.1002/jobm.202300682
Beerelli Deepak Reddy, Birendra Kumar, Sangita Sahni, Gummudala Yashaswini, Somala Karthik, Morthala Shankara Sai Reddy, Rajeev Kumar, Udayan Mukherjee
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引用次数: 0

摘要

在印度进行的一项研究中,从鸽子豆种植区收集了 50 个镰刀菌分离物,并对其文化和形态特征进行了广泛研究。这些分离物在生长速度、菌丝生长模式、颜色、分带、色素沉着、孢子大小和隔膜等性状上表现出明显的差异。随后,选择了 30 个分离株对 8 个鸽子豆基因型进行致病性测试。结果显示了不同的反应,其中四个基因型(ICP8858、ICP8859、ICP8862 和 BDN-2)表现出不同的反应,而 ICP9174 和 ICP8863 始终表现出抗性,ICP2376 和 BAHAR 对枯萎病仍然易感。为了研究镰刀菌分离物与鸽子豆寄主差异(HDs)之间的交互作用,进行了加性主效应和乘性交互作用分析。大部分病害发生率的变化(75.54%)归因于 HD 的影响,而镰刀菌分离株的影响仅占 1.99%。分离株与 HD 之间的交互作用(I × HD)占总变异的 21.95%,小于 HD,但大于 I。根据 HD 反应,分离株被分为 9 个变异株,在鸽子豆种植州的分布各不相同,其中变异株 2 和 3 在几个地区很普遍。这种多样性突出表明,有必要培育针对特定地区的抗枯萎病鸽子豆栽培品种。此外,通过核糖体 DNA 内部转录间隔区和翻译伸长因子 1-α 基因测序对 23 个代表性分离物进行遗传分析,发现了三个主要群集:Fusarium udum、Fusarium solani 和 Fusarium equiseti。这些发现为开发特定地点的抗枯萎病鸽子豆栽培品种和加强病害管理策略提供了潜力。
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Genetic diversity and population structure of Fusarium udum in India and its correlation with pigeonpea wilt incidence

In a study conducted in India, 50 Fusarium isolates were collected from pigeonpea growing regions and extensively examined for their cultural and morphological characteristics. These isolates exhibited significant variations in traits including growth rate, mycelial growth patterns, color, zonation, pigmentation, spore size, and septation. Subsequently, 30 isolates were chosen for pathogenicity testing on eight pigeonpea genotypes. Results showed distinct reactions, with four genotypes displaying differential responses (ICP8858, ICP8859, ICP8862, and BDN-2), while ICP9174 and ICP8863 consistently exhibited resistance and ICP2376 and BAHAR remained susceptible to wilt disease. To study the interaction between Fusarium isolates and pigeonpea host differentials (HDs), an additive main effects and multiplicative interaction analysis was conducted. The majority of disease incidence variation (75.54%) was attributed to HD effects, while Fusarium isolate effects accounted for only 1.99%. The interaction between Isolates and HDs (I × HD) contributed 21.95% to the total variation, being smaller than HD but larger than I. Based on HD reactions, isolates were classified into nine variants, showing varying distributions across pigeonpea growing states, with variants 2 and 3 being prevalent in several regions. This diversity underscores the need for location-specific wilt-resistant pigeonpea cultivars. Furthermore, genetic analysis of 23 representative isolates, through internal transcribed spacer region of ribosomal DNA and translation elongation factor 1-α gene sequencing, revealed three major clusters: Fusarium udum, Fusarium solani, and Fusarium equiseti. These findings hold potential for developing location-specific wilt-resistant pigeonpea cultivars and enhancing disease management strategies.

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来源期刊
Journal of Basic Microbiology
Journal of Basic Microbiology 生物-微生物学
CiteScore
6.10
自引率
0.00%
发文量
134
审稿时长
1.8 months
期刊介绍: The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).
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