用于原核系统工程的 CRISPR 工具:最新进展和新应用

IF 7.6 2区 工程技术 Q1 CHEMISTRY, APPLIED Annual review of chemical and biomolecular engineering Pub Date : 2024-04-10 DOI:10.1146/annurev-chembioeng-100522-114706
Diego Alba Burbano, Cholpisit Kiattisewee, Ava V. Karanjia, Ryan A.L. Cardiff, Ian D. Faulkner, Widianti Sugianto, James M. Carothers
{"title":"用于原核系统工程的 CRISPR 工具:最新进展和新应用","authors":"Diego Alba Burbano, Cholpisit Kiattisewee, Ava V. Karanjia, Ryan A.L. Cardiff, Ian D. Faulkner, Widianti Sugianto, James M. Carothers","doi":"10.1146/annurev-chembioeng-100522-114706","DOIUrl":null,"url":null,"abstract":"In the past decades, the broad selection of CRISPR-Cas systems has revolutionized biotechnology by enabling multimodal genetic manipulation in diverse organisms. Rooted in a molecular engineering perspective, we recapitulate the different CRISPR components and how they can be designed for specific genetic engineering applications. We first introduce the repertoire of Cas proteins and tethered effectors used to program new biological functions through gene editing and gene regulation. We review current guide RNA (gRNA) design strategies and computational tools and how CRISPR-based genetic circuits can be constructed through regulated gRNA expression. Then, we present recent advances in CRISPR-based biosensing, bioproduction, and biotherapeutics across in vitro and in vivo prokaryotic systems. Finally, we discuss forthcoming applications in prokaryotic CRISPR technology that will transform synthetic biology principles in the near future.","PeriodicalId":8234,"journal":{"name":"Annual review of chemical and biomolecular engineering","volume":null,"pages":null},"PeriodicalIF":7.6000,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications\",\"authors\":\"Diego Alba Burbano, Cholpisit Kiattisewee, Ava V. Karanjia, Ryan A.L. Cardiff, Ian D. Faulkner, Widianti Sugianto, James M. Carothers\",\"doi\":\"10.1146/annurev-chembioeng-100522-114706\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In the past decades, the broad selection of CRISPR-Cas systems has revolutionized biotechnology by enabling multimodal genetic manipulation in diverse organisms. Rooted in a molecular engineering perspective, we recapitulate the different CRISPR components and how they can be designed for specific genetic engineering applications. We first introduce the repertoire of Cas proteins and tethered effectors used to program new biological functions through gene editing and gene regulation. We review current guide RNA (gRNA) design strategies and computational tools and how CRISPR-based genetic circuits can be constructed through regulated gRNA expression. Then, we present recent advances in CRISPR-based biosensing, bioproduction, and biotherapeutics across in vitro and in vivo prokaryotic systems. Finally, we discuss forthcoming applications in prokaryotic CRISPR technology that will transform synthetic biology principles in the near future.\",\"PeriodicalId\":8234,\"journal\":{\"name\":\"Annual review of chemical and biomolecular engineering\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":7.6000,\"publicationDate\":\"2024-04-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annual review of chemical and biomolecular engineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1146/annurev-chembioeng-100522-114706\",\"RegionNum\":2,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annual review of chemical and biomolecular engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1146/annurev-chembioeng-100522-114706","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0

摘要

在过去的几十年里,CRISPR-Cas 系统的广泛应用为生物技术带来了革命性的变化,使多种生物的多模式遗传操作成为可能。我们从分子工程的角度出发,概述了 CRISPR 的不同组成部分,以及如何将它们设计用于特定的基因工程应用。我们首先介绍了用于通过基因编辑和基因调控来编程新生物功能的 Cas 蛋白和系留效应器。我们回顾了当前的引导 RNA(gRNA)设计策略和计算工具,以及如何通过调控 gRNA 的表达来构建基于 CRISPR 的基因回路。然后,我们介绍了基于 CRISPR 的生物传感、生物生产和生物治疗在体外和体内原核系统中的最新进展。最后,我们将讨论原核生物 CRISPR 技术即将出现的应用,这些应用将在不久的将来改变合成生物学原理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
CRISPR Tools for Engineering Prokaryotic Systems: Recent Advances and New Applications
In the past decades, the broad selection of CRISPR-Cas systems has revolutionized biotechnology by enabling multimodal genetic manipulation in diverse organisms. Rooted in a molecular engineering perspective, we recapitulate the different CRISPR components and how they can be designed for specific genetic engineering applications. We first introduce the repertoire of Cas proteins and tethered effectors used to program new biological functions through gene editing and gene regulation. We review current guide RNA (gRNA) design strategies and computational tools and how CRISPR-based genetic circuits can be constructed through regulated gRNA expression. Then, we present recent advances in CRISPR-based biosensing, bioproduction, and biotherapeutics across in vitro and in vivo prokaryotic systems. Finally, we discuss forthcoming applications in prokaryotic CRISPR technology that will transform synthetic biology principles in the near future.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Annual review of chemical and biomolecular engineering
Annual review of chemical and biomolecular engineering CHEMISTRY, APPLIED-ENGINEERING, CHEMICAL
CiteScore
16.00
自引率
0.00%
发文量
25
期刊介绍: The Annual Review of Chemical and Biomolecular Engineering aims to provide a perspective on the broad field of chemical (and related) engineering. The journal draws from disciplines as diverse as biology, physics, and engineering, with development of chemical products and processes as the unifying theme.
期刊最新文献
Reassessing the Standard Chemotaxis Framework for Understanding Biased Migration in Helicobacter pylori. Models for Decarbonization in the Chemical Industry. Introduction. Will Hydrogen Be a New Natural Gas? Hydrogen Integration in Natural Gas Grids. Fluid Ejections in Nature
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1