红参提取物可抑制脂多糖诱导的小鼠血小板-白细胞聚集

IF 6.8 2区 医学 Q1 CHEMISTRY, MEDICINAL Journal of Ginseng Research Pub Date : 2024-04-07 DOI:10.1016/j.jgr.2024.03.009
Yuan Yee Lee , Sung Dae Kim , Jin-Kyu Park , Won-Jae Lee , Jee Eun Han , Min-Soo Seo , Min-Goo Seo , Seulgi Bae , Dongmi Kwak , Evelyn Saba , Man Hee Rhee
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引用次数: 0

摘要

血小板-白细胞聚集体(PLA)在心血管疾病和败血症中发挥着重要作用。红参提取物(RGE)的抗血小板和抗炎活性已得到广泛研究。然而,红参提取物对聚乳酸的潜在抑制作用尚未得到研究。给六周大的 ICR 小鼠口服 RGE 7 天,然后腹腔注射 15 毫克/千克脂多糖。24 小时后对小鼠实施安乐死,并采集血液样本进行进一步分析。利用流式细胞术对血小板聚集体和血小板-中性粒细胞聚集体(PNA)进行分类。使用共聚焦显微镜对 PNAs 进行验证。用扫描电子显微镜观察血小板和白细胞的形态变化。使用酶联免疫吸附试验检测了组织因子(TF)和血小板因子 4(PF4)的表达。在 LPS 诱导下,活化血小板、PLA 和 PNA 的数量显著增加。使用 200 和 400 毫克/千克的 RGE 可降低血小板活化。此外,PLA 和 PNA 的数量也减少了。脓毒症小鼠的血液中可见 PNAs,使用 400 毫克/千克的 RGE 治疗可减轻这一现象。从形态上看,败血症会导致血小板活化和血液中纤维蛋白的形成。使用 RGE 治疗后,这种现象有所减少。经 RGE 治疗后,败血症引起的血浆中 TF 和 PF4 含量增加也有所减少。这项研究表明,RGE 是一种潜在的疗法,它能减少血小板的活化,并抑制 PLA 和 PNA 的形成。RGE 的详细抑制机制有待研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Red ginseng extract inhibits lipopolysaccharide-induced platelet–leukocyte aggregates in mice

Background

Platelet–leukocyte aggregates (PLAs) play important roles in cardiovascular disease and sepsis. Red ginseng extract (RGE) has been well-studied for its antiplatelet and anti-inflammatory activities. However, the potential inhibitory effects of RGE on PLA have not been investigated.

Methods

Six-week-old ICR mice were given oral gavage of RGE for 7 days, followed by an intraperitoneal injection of 15 mg/kg of lipopolysaccharide. Mice were euthanized 24 h later, and blood samples were collected for further analysis. Flow cytometry was utilized to sort populations of PLAs and platelet–neutrophil aggregates (PNAs). By using confocal microscopy, PNAs were validated. Morphological changes in platelets and leukocytes were visualized with scanning electron microscopy. Expressions of tissue factor (TF) and platelet factor 4 (PF4) were investigated using enzyme-linked immunosorbent assay.

Results

Populations of activated platelets, PLAs and PNAs, were significantly increased with LPS-induction. Treatment with 200 and 400 mg/kg of RGE decreased platelet activation. Moreover, the populations of PLAs and PNAs were reduced. PNAs were visible in the blood of septic mice, and this was attenuated by treatment with 400 mg/kg of RGE. Morphologically, sepsisinduced platelet activation and fibrin formation in the blood. This was reduced with RGE treatment. Sepsis-induced increase in the plasma levels of TF and PF4 was also reduced with RGE treatment.

Conclusion

This study shows that RGE is a potential therapeutic that reduces the activation of platelets and targets PLA and PNA formation. Detailed inhibitory mechanisms of RGE should be studied.

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来源期刊
Journal of Ginseng Research
Journal of Ginseng Research CHEMISTRY, MEDICINAL-INTEGRATIVE & COMPLEMENTARY MEDICINE
CiteScore
11.40
自引率
9.50%
发文量
111
审稿时长
6-12 weeks
期刊介绍: Journal of Ginseng Research (JGR) is an official, open access journal of the Korean Society of Ginseng and is the only international journal publishing scholarly reports on ginseng research in the world. The journal is a bimonthly peer-reviewed publication featuring high-quality studies related to basic, pre-clinical, and clinical researches on ginseng to reflect recent progresses in ginseng research. JGR publishes papers, either experimental or theoretical, that advance our understanding of ginseng science, including plant sciences, biology, chemistry, pharmacology, toxicology, pharmacokinetics, veterinary medicine, biochemistry, manufacture, and clinical study of ginseng since 1976. It also includes the new paradigm of integrative research, covering alternative medicinal approaches. Article types considered for publication include review articles, original research articles, and brief reports. JGR helps researchers to understand mechanisms for traditional efficacy of ginseng and to put their clinical evidence together. It provides balanced information on basic science and clinical applications to researchers, manufacturers, practitioners, teachers, scholars, and medical doctors.
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