部分纯化的漆酶在聚羟基烷酸（PHA）微珠上的固定化及其在儿茶酚生物降解中的应用

IF 2.8 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of chemical technology and biotechnology Pub Date : 2024-04-15 DOI:10.1002/jctb.7654

Kirti Garg, Rutika Sehgal, Divya Sharma, Reena Gupta

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引用次数: 0

摘要

新的快速经济发展的一个重要组成部分是创造可生物降解的聚合物。其中一种生物聚合物是聚羟基烷酸酯（PHA）。这种可生物降解、热塑性和不溶于水的储存聚合物可从可持续碳源中生成。PHA 可通过固定酶来有效处理废水。为了消除微污染物和许多酚类化合物，漆酶被认为是一种潜在的酶。因此，它在水净化过程中非常有效。不过，要使漆酶发挥高效催化作用，必须进行适当的固定。固定化程序提高了漆酶在重复使用性、温度、pH 值和储存方面的稳定性，使其优于游离漆酶。

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Immobilization of partially purified laccase on polyhydroxyalkanoate (PHA) microbeads and its application in biodegradation of catechol

BACKGROUND

One significant component of new, rapid economic development is the creation of biodegradable polymers. One of these biopolymers is polyhydroxyalkanoate (PHA). This biodegradable, thermoplastic and water-insoluble storage polymer can be generated from sustainable carbon sources. PHA may be effectively used for treating wastewaters by immobilizing enzymes. For eliminating micropollutants along with many phenolic compounds, laccase is considered as a potential enzyme. Hence, it has been known to be highly effective for water purification procedures. However, adequate immobilization is required for laccase to perform efficient catalysis. The immobilization procedure increases laccase stability with respect to reusability, temperature, pH and storage, making it superior to free laccase.

RESULT

In the present study, laccase was partially purified from Beauveria pseudobassiana PHF4 by gel filtration chromatography and further immobilized on PHA microbeads. The crude extract displayed specific activity of 19.08 U mg⁻¹ and PHA microbeads demonstrated immobilization efficiency of 77.44%. Characterization of PHA microbeads by scanning electron microscopy showed an increase in their size from 3–5 μm to 5–6 μm after the immobilization. Furthermore, catechol biodegradation by immobilized laccase was analyzed using UV–visible spectrophotometry (84.25% in 10 h), which was also confirmed by high-performance liquid chromatography (83.65% in 24 h).

CONCLUSION

Iimmobilizing laccase on PHA microbeads can help to address the pollution and environmental damage that catechol and similar chemicals cause also in an ecofriendly manner. © 2024 Society of Chemical Industry (SCI).

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来源期刊

Journal of chemical technology and biotechnology 工程技术-工程：化工

CiteScore

7.00

自引率

5.90%

发文量

268

审稿时长

1.7 months

期刊介绍： Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.