Ulises Amador‐Marrero, Silvie Dumas, Sergio F. Martínez‐Díaz, Mauricio Contreras‐Olguín, Laura Flores‐Montijo, Bárbara González‐Acosta, Ricardo Vázquez‐Juárez, Norma Estrada, Patricia J. Ramírez‐Arenas
{"title":"冠突伪尾柱虫稚鱼的细菌群落以及稚鱼作为向鱼类幼体提供早期益生菌的载体的潜力","authors":"Ulises Amador‐Marrero, Silvie Dumas, Sergio F. Martínez‐Díaz, Mauricio Contreras‐Olguín, Laura Flores‐Montijo, Bárbara González‐Acosta, Ricardo Vázquez‐Juárez, Norma Estrada, Patricia J. Ramírez‐Arenas","doi":"10.1002/naaq.10331","DOIUrl":null,"url":null,"abstract":"ObjectiveThe copepod <jats:italic>Parvocalanus crassirostris</jats:italic> has recently increased its use as live food in marine fish hatcheries.MethodsTo investigate the bacterial community of <jats:italic>P. crassirostris</jats:italic> nauplii and explore the potential use of nauplii as a vector for probiotics, adult copepods were reared with or without a probiotic consortium (<jats:italic>Bacillus</jats:italic> spp. and <jats:italic>Lactobacillus</jats:italic> spp.). Nauplii from both treatments were either enriched with probiotics or not, resulting in four treatments: control rearing and nonenriched, control rearing and probiotic enriched, probiotic rearing and nonenriched, and probiotic rearing probiotic enriched. A next‐generation sequencing analysis of the 16S ribosomal RNA gene was performed.ResultThe bacterial community of nauplii without probiotics was dominated by the phyla Proteobacteria (mean ± standard deviation = 51.5 ± 8.0%) and Bacteroidetes (27.6 ± 6.7%); from these phyla, the most abundant orders were Rhodobacterales (21.8 ± 2.0%), Flavobacteriales (13.5 ± 9.4%), Chitinophagales (9.7 ± 1.8%), Alteromonadales (9.5 ± 4.0%), and Burkholderiales (3.8 ± 0.8%). Opportunistic pathogenic bacterial genera, such as <jats:italic>Vibrio</jats:italic> spp. and <jats:italic>Pseudomonas</jats:italic> spp., were detected at very low relative abundances (<1.2%), indicating that <jats:italic>P. crassirostris</jats:italic> nauplii could be considered a safe microbiological live feed. Furthermore, <jats:italic>P. crassirostris</jats:italic> nauplii were a good vector for probiotics delivery, as the adhesion of probiotics on the copepod exoskeleton was successful. <jats:italic>Bacillus</jats:italic> spp. abundance was 5–6× greater than the abundance of <jats:italic>Lactobacillus</jats:italic> spp. in <jats:italic>P. crassirostris</jats:italic> nauplii. The highest mean values of probiotics relative abundance (<jats:italic>Bacillus</jats:italic> spp. 41 ± 6.0%, <jats:italic>Lactobacillus</jats:italic> spp. 6.0 ± 3.0%) were observed in nauplii that were reared with probiotics and also enriched with probiotics.Conclusion<jats:italic>Parvocalanus crassirostris</jats:italic> nauplii stages I–II appear to be good vectors to provide probiotics to first‐feeding fish larvae.","PeriodicalId":19258,"journal":{"name":"North American Journal of Aquaculture","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bacterial community of Parvocalanus crassirostris nauplii and the potential of nauplii as a vector for early probiotic supply to fish larvae\",\"authors\":\"Ulises Amador‐Marrero, Silvie Dumas, Sergio F. Martínez‐Díaz, Mauricio Contreras‐Olguín, Laura Flores‐Montijo, Bárbara González‐Acosta, Ricardo Vázquez‐Juárez, Norma Estrada, Patricia J. Ramírez‐Arenas\",\"doi\":\"10.1002/naaq.10331\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ObjectiveThe copepod <jats:italic>Parvocalanus crassirostris</jats:italic> has recently increased its use as live food in marine fish hatcheries.MethodsTo investigate the bacterial community of <jats:italic>P. crassirostris</jats:italic> nauplii and explore the potential use of nauplii as a vector for probiotics, adult copepods were reared with or without a probiotic consortium (<jats:italic>Bacillus</jats:italic> spp. and <jats:italic>Lactobacillus</jats:italic> spp.). Nauplii from both treatments were either enriched with probiotics or not, resulting in four treatments: control rearing and nonenriched, control rearing and probiotic enriched, probiotic rearing and nonenriched, and probiotic rearing probiotic enriched. A next‐generation sequencing analysis of the 16S ribosomal RNA gene was performed.ResultThe bacterial community of nauplii without probiotics was dominated by the phyla Proteobacteria (mean ± standard deviation = 51.5 ± 8.0%) and Bacteroidetes (27.6 ± 6.7%); from these phyla, the most abundant orders were Rhodobacterales (21.8 ± 2.0%), Flavobacteriales (13.5 ± 9.4%), Chitinophagales (9.7 ± 1.8%), Alteromonadales (9.5 ± 4.0%), and Burkholderiales (3.8 ± 0.8%). Opportunistic pathogenic bacterial genera, such as <jats:italic>Vibrio</jats:italic> spp. and <jats:italic>Pseudomonas</jats:italic> spp., were detected at very low relative abundances (<1.2%), indicating that <jats:italic>P. crassirostris</jats:italic> nauplii could be considered a safe microbiological live feed. Furthermore, <jats:italic>P. crassirostris</jats:italic> nauplii were a good vector for probiotics delivery, as the adhesion of probiotics on the copepod exoskeleton was successful. <jats:italic>Bacillus</jats:italic> spp. abundance was 5–6× greater than the abundance of <jats:italic>Lactobacillus</jats:italic> spp. in <jats:italic>P. crassirostris</jats:italic> nauplii. The highest mean values of probiotics relative abundance (<jats:italic>Bacillus</jats:italic> spp. 41 ± 6.0%, <jats:italic>Lactobacillus</jats:italic> spp. 6.0 ± 3.0%) were observed in nauplii that were reared with probiotics and also enriched with probiotics.Conclusion<jats:italic>Parvocalanus crassirostris</jats:italic> nauplii stages I–II appear to be good vectors to provide probiotics to first‐feeding fish larvae.\",\"PeriodicalId\":19258,\"journal\":{\"name\":\"North American Journal of Aquaculture\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-04-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"North American Journal of Aquaculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1002/naaq.10331\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"North American Journal of Aquaculture","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/naaq.10331","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FISHERIES","Score":null,"Total":0}
Bacterial community of Parvocalanus crassirostris nauplii and the potential of nauplii as a vector for early probiotic supply to fish larvae
ObjectiveThe copepod Parvocalanus crassirostris has recently increased its use as live food in marine fish hatcheries.MethodsTo investigate the bacterial community of P. crassirostris nauplii and explore the potential use of nauplii as a vector for probiotics, adult copepods were reared with or without a probiotic consortium (Bacillus spp. and Lactobacillus spp.). Nauplii from both treatments were either enriched with probiotics or not, resulting in four treatments: control rearing and nonenriched, control rearing and probiotic enriched, probiotic rearing and nonenriched, and probiotic rearing probiotic enriched. A next‐generation sequencing analysis of the 16S ribosomal RNA gene was performed.ResultThe bacterial community of nauplii without probiotics was dominated by the phyla Proteobacteria (mean ± standard deviation = 51.5 ± 8.0%) and Bacteroidetes (27.6 ± 6.7%); from these phyla, the most abundant orders were Rhodobacterales (21.8 ± 2.0%), Flavobacteriales (13.5 ± 9.4%), Chitinophagales (9.7 ± 1.8%), Alteromonadales (9.5 ± 4.0%), and Burkholderiales (3.8 ± 0.8%). Opportunistic pathogenic bacterial genera, such as Vibrio spp. and Pseudomonas spp., were detected at very low relative abundances (<1.2%), indicating that P. crassirostris nauplii could be considered a safe microbiological live feed. Furthermore, P. crassirostris nauplii were a good vector for probiotics delivery, as the adhesion of probiotics on the copepod exoskeleton was successful. Bacillus spp. abundance was 5–6× greater than the abundance of Lactobacillus spp. in P. crassirostris nauplii. The highest mean values of probiotics relative abundance (Bacillus spp. 41 ± 6.0%, Lactobacillus spp. 6.0 ± 3.0%) were observed in nauplii that were reared with probiotics and also enriched with probiotics.ConclusionParvocalanus crassirostris nauplii stages I–II appear to be good vectors to provide probiotics to first‐feeding fish larvae.
期刊介绍:
The North American Journal of Aquaculture publishes papers on new research and practical experience in all areas of intensive and extensive fish culture. Topics include broodstock selection and spawning, nutrition and feeding, health and water quality, facilities and production technology, and the management of ponds, pens, and raceways.
The journal will consider papers dealing with ways to improve the husbandry of any aquatic species—marine or freshwater, vertebrate or invertebrate—raised for commercial, scientific, recreational, enhancement, or restoration purposes that may be of interest to practitioners in North America. Its scope includes both basic and applied science, but applied scientific endeavors—including practical experiences, descriptive studies, and other nontraditional, but pertinent works—are emphasized.