{"title":"活细胞中的 DNA 程序货物分配系统","authors":"Yuhan Wei, Yueyue Feng, Xiaoliang Chen, Wenhe Ma, Kaizhe Wang, Qian Li, Jiang Li, Lihua Wang, Chunhai Fan, Ying Zhu","doi":"10.31635/ccschem.024.202404080","DOIUrl":null,"url":null,"abstract":"The functionalization of living cells, both internally and externally, transforming them into micromachines with specified functions, holds significant potential in fields such as biosensing, biocomputing, and intelligent theranostics. However, due to the complexity and dynamic nature of living cells, it remains challenging to allocate exogenous functional materials to specific locations within the cell or on its surface and maintain their positions stable for a reasonable period. Here, we devise a DNA-programmed cargo distributing system (DCD), capable of distributing functional modules to the cell membrane or within the cell as needed. This system includes an amphiphilic DNA structure for determining the destination of the cargo and a DNA connector carried on it for recognizing the DNA-encoded cargo. We test three different morphologies of amphiphilic DNA structures and find that their efficiencies in cell surface retention and cell internalization significantly varied, enabling the distribution of nanoparticle cargos on the cell membrane and within the cell in distinct proportions. Their positions can remain stable for at least 6 hours. Moreover, this allocation method shows specificity, which minimizes the deployment of mismatched cargo. This method provides new tools for the modular construction of cellular micromachines.\n<figure><img alt=\"\" data-lg-src=\"/cms/asset/00f94d08-4a6d-4e45-8bc5-ea2724a40021/keyimage.jpg\" data-src=\"/cms/asset/8d08d50e-b62a-40c8-a596-7bcde67e39b2/keyimage.jpg\" src=\"/specs/ux3/releasedAssets/images/loader-7e60691fbe777356dc81ff6d223a82a6.gif\"/><ul>\n<li>Download figure</li>\n<li>Download PowerPoint</li>\n</ul>\n</figure>","PeriodicalId":9810,"journal":{"name":"CCS Chemistry","volume":null,"pages":null},"PeriodicalIF":9.4000,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A DNA-programmed Cargo Distributing System in Living Cells\",\"authors\":\"Yuhan Wei, Yueyue Feng, Xiaoliang Chen, Wenhe Ma, Kaizhe Wang, Qian Li, Jiang Li, Lihua Wang, Chunhai Fan, Ying Zhu\",\"doi\":\"10.31635/ccschem.024.202404080\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The functionalization of living cells, both internally and externally, transforming them into micromachines with specified functions, holds significant potential in fields such as biosensing, biocomputing, and intelligent theranostics. However, due to the complexity and dynamic nature of living cells, it remains challenging to allocate exogenous functional materials to specific locations within the cell or on its surface and maintain their positions stable for a reasonable period. Here, we devise a DNA-programmed cargo distributing system (DCD), capable of distributing functional modules to the cell membrane or within the cell as needed. This system includes an amphiphilic DNA structure for determining the destination of the cargo and a DNA connector carried on it for recognizing the DNA-encoded cargo. We test three different morphologies of amphiphilic DNA structures and find that their efficiencies in cell surface retention and cell internalization significantly varied, enabling the distribution of nanoparticle cargos on the cell membrane and within the cell in distinct proportions. Their positions can remain stable for at least 6 hours. Moreover, this allocation method shows specificity, which minimizes the deployment of mismatched cargo. 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引用次数: 0
摘要
活细胞的内部和外部功能化,将其转化为具有特定功能的微型机械,在生物传感、生物计算和智能治疗学等领域具有巨大潜力。然而,由于活细胞的复杂性和动态性,如何将外源功能材料分配到细胞内或细胞表面的特定位置,并在合理的时间内保持其位置稳定,仍然具有挑战性。在这里,我们设计了一种 DNA 编程的货物分配系统(DCD),能够根据需要将功能模块分配到细胞膜或细胞内。该系统包括一个用于确定货物目的地的两亲 DNA 结构和一个用于识别 DNA 编码货物的 DNA 连接器。我们测试了三种不同形态的两亲 DNA 结构,发现它们在细胞表面保留和细胞内化方面的效率有显著差异,从而使纳米粒子货物能够以不同的比例分布在细胞膜上和细胞内。它们的位置至少可以保持稳定 6 小时。此外,这种分配方法还具有特异性,可最大限度地减少不匹配货物的部署。这种方法为模块化构建细胞微型机械提供了新的工具。 下载图下载 PowerPoint
A DNA-programmed Cargo Distributing System in Living Cells
The functionalization of living cells, both internally and externally, transforming them into micromachines with specified functions, holds significant potential in fields such as biosensing, biocomputing, and intelligent theranostics. However, due to the complexity and dynamic nature of living cells, it remains challenging to allocate exogenous functional materials to specific locations within the cell or on its surface and maintain their positions stable for a reasonable period. Here, we devise a DNA-programmed cargo distributing system (DCD), capable of distributing functional modules to the cell membrane or within the cell as needed. This system includes an amphiphilic DNA structure for determining the destination of the cargo and a DNA connector carried on it for recognizing the DNA-encoded cargo. We test three different morphologies of amphiphilic DNA structures and find that their efficiencies in cell surface retention and cell internalization significantly varied, enabling the distribution of nanoparticle cargos on the cell membrane and within the cell in distinct proportions. Their positions can remain stable for at least 6 hours. Moreover, this allocation method shows specificity, which minimizes the deployment of mismatched cargo. This method provides new tools for the modular construction of cellular micromachines.
期刊介绍:
CCS Chemistry, the flagship publication of the Chinese Chemical Society, stands as a leading international chemistry journal based in China. With a commitment to global outreach in both contributions and readership, the journal operates on a fully Open Access model, eliminating subscription fees for contributing authors. Issued monthly, all articles are published online promptly upon reaching final publishable form. Additionally, authors have the option to expedite the posting process through Immediate Online Accepted Article posting, making a PDF of their accepted article available online upon journal acceptance.