肺部压力变化产生的溶解气体会引起人体外周血的免疫反应

IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Bioengineering & Translational Medicine Pub Date : 2024-04-16 DOI:10.1002/btm2.10657
Abigail G. Harrell, Stephen R. Thom, C. Wyatt Shields IV
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摘要

传统理论认为,减压病(DCS)是由血管和/或组织中的氮气气泡成核引起的;然而,气泡的数量与减压病的严重程度并不相关。由于免疫细胞会对化学和环境线索做出反应,我们假设溶解气体分压的升高会导致肺泡血管中的免疫细胞表型异常。为了验证这一假设,我们测量了使用原代肺泡细胞和微血管细胞建立的人肺芯片装置内的免疫反应。装置加压至 1.0 或 3.5 atm,周围为正常肺泡空气或减氧空气。中性粒细胞、单核细胞和树突状细胞的表型分析以及多重酶联免疫吸附试验表明,免疫反应在 1 小时内发生,正常肺泡空气(即高压氧和氮气)会带来更大的免疫激活。这项研究有力地表明,在局部压力升高的情况下启动的先天性免疫细胞反应是导致 DCS 的病因之一。
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Dissolved gases from pressure changes in the lungs elicit an immune response in human peripheral blood

Conventional dogma suggests that decompression sickness (DCS) is caused by nitrogen bubble nucleation in the blood vessels and/or tissues; however, the abundance of bubbles does not correlate with DCS severity. Since immune cells respond to chemical and environmental cues, we hypothesized that the elevated partial pressures of dissolved gases drive aberrant immune cell phenotypes in the alveolar vasculature. To test this hypothesis, we measured immune responses within human lung-on-a-chip devices established with primary alveolar cells and microvascular cells. Devices were pressurized to 1.0 or 3.5 atm and surrounded by normal alveolar air or oxygen-reduced air. Phenotyping of neutrophils, monocytes, and dendritic cells as well as multiplexed ELISA revealed that immune responses occur within 1 h and that normal alveolar air (i.e., hyperbaric oxygen and nitrogen) confer greater immune activation. This work strongly suggests innate immune cell reactions initiated at elevated partial pressures contribute to the etiology of DCS.

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来源期刊
Bioengineering & Translational Medicine
Bioengineering & Translational Medicine Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
8.40
自引率
4.10%
发文量
150
审稿时长
12 weeks
期刊介绍: Bioengineering & Translational Medicine, an official, peer-reviewed online open-access journal of the American Institute of Chemical Engineers (AIChE) and the Society for Biological Engineering (SBE), focuses on how chemical and biological engineering approaches drive innovative technologies and solutions that impact clinical practice and commercial healthcare products.
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