{"title":"日粮中铜的含量会影响放牧蒙古绵羊的矿物质吸收率、瘤胃微生物组成和代谢物","authors":"Lingbo Meng, Xiwei Jin, Zhi Qi, Lan Mi","doi":"10.1016/j.anifeedsci.2024.115970","DOIUrl":null,"url":null,"abstract":"<div><p>Copper (Cu) is a critical limiting factor in grazing livestock production in Inner Mongolia. This study investigated the effects of Cu-deficient and Cu-supplemented diets on production metrics, nutrient digestion and absorption, ruminal microbiota, and metabolites in grazing Mongolian sheep. A total of 28 individually-housed Mongolian sheep were randomly assigned to a Cu-deficient feeding group (LCu) and a Cu-deficient feeding control group (LCG), supplemented with a multi-nutrient salt containing 0 mg Cu and 14.3 mg Cu, respectively, for 53 continuous days. Following this, a 7-day digestion test was conducted, after which seven sheep from each group were randomly slaughtered. The remaining sheep in LCu and LCG transitioned to a Cu-supplemented feeding group (SCu) and a Cu-supplemented feeding control group (SCG), receiving multi-nutrient salts with 43.0 mg Cu and 14.3 mg Cu, respectively. This phase entailed a 34-day diet acclimatization period, a 7-day digestion test, and eventual slaughter. The LCu diet significantly decreased the ruminal Cu and sulfur (S) content, as well as Cu apparent absorbability, compared to the LCG group (<em>P<</em>0.05). Conversely, SCu significantly increased the ruminal Cu content and the apparent absorbability of phosphorus (P), S, potassium (K), calcium (Ca), manganese (Mn), iron (Fe), Cu and zinc (Zn) compared to the SCG group (<em>P<</em>0.05). Amplicon sequencing analysis revealed a significant downregulation of <em>Alistipes</em> and <em>Barnesiella</em> (<em>P<</em>0.05) in the LCu group. Metabolomic analyses supported an elevation in ruminal citrulline and L-ornithine levels, affecting amino acid metabolic pathways in the LCu group (<em>P<</em>0.05). In the SCu group, there was a significant increase in the relative abundance of <em>Saccharofermentans</em> and <em>Ruminococcus</em> (<em>P<</em>0.05), with metabolomic analysis revealed elevated levels of nucleic acid and purine analog affecting purine metabolic pathways (<em>P<</em>0.05). Notably, neither treatment had a significant impact production metrics, rumen fermentation parameters, or the apparent digestibility of crude protein, acid detergent fiber and neutral detergent fiber (<em>P></em>0.05). In conclusion, a Cu-deficient diet impairs Cu absorption and nutrient catabolism in grazing Mongolian sheep, while Cu supplementation effectively alleviated these deficiencies and enhances the absorption of various minerals.</p></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0377840124000981/pdfft?md5=16146e3740a3faf9faa01929c7d721aa&pid=1-s2.0-S0377840124000981-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Dietary copper levels affect mineral absorbability, rumen microbial composition and metabolites of the grazing Mongolian sheep\",\"authors\":\"Lingbo Meng, Xiwei Jin, Zhi Qi, Lan Mi\",\"doi\":\"10.1016/j.anifeedsci.2024.115970\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Copper (Cu) is a critical limiting factor in grazing livestock production in Inner Mongolia. This study investigated the effects of Cu-deficient and Cu-supplemented diets on production metrics, nutrient digestion and absorption, ruminal microbiota, and metabolites in grazing Mongolian sheep. A total of 28 individually-housed Mongolian sheep were randomly assigned to a Cu-deficient feeding group (LCu) and a Cu-deficient feeding control group (LCG), supplemented with a multi-nutrient salt containing 0 mg Cu and 14.3 mg Cu, respectively, for 53 continuous days. Following this, a 7-day digestion test was conducted, after which seven sheep from each group were randomly slaughtered. The remaining sheep in LCu and LCG transitioned to a Cu-supplemented feeding group (SCu) and a Cu-supplemented feeding control group (SCG), receiving multi-nutrient salts with 43.0 mg Cu and 14.3 mg Cu, respectively. This phase entailed a 34-day diet acclimatization period, a 7-day digestion test, and eventual slaughter. The LCu diet significantly decreased the ruminal Cu and sulfur (S) content, as well as Cu apparent absorbability, compared to the LCG group (<em>P<</em>0.05). Conversely, SCu significantly increased the ruminal Cu content and the apparent absorbability of phosphorus (P), S, potassium (K), calcium (Ca), manganese (Mn), iron (Fe), Cu and zinc (Zn) compared to the SCG group (<em>P<</em>0.05). Amplicon sequencing analysis revealed a significant downregulation of <em>Alistipes</em> and <em>Barnesiella</em> (<em>P<</em>0.05) in the LCu group. Metabolomic analyses supported an elevation in ruminal citrulline and L-ornithine levels, affecting amino acid metabolic pathways in the LCu group (<em>P<</em>0.05). In the SCu group, there was a significant increase in the relative abundance of <em>Saccharofermentans</em> and <em>Ruminococcus</em> (<em>P<</em>0.05), with metabolomic analysis revealed elevated levels of nucleic acid and purine analog affecting purine metabolic pathways (<em>P<</em>0.05). Notably, neither treatment had a significant impact production metrics, rumen fermentation parameters, or the apparent digestibility of crude protein, acid detergent fiber and neutral detergent fiber (<em>P></em>0.05). In conclusion, a Cu-deficient diet impairs Cu absorption and nutrient catabolism in grazing Mongolian sheep, while Cu supplementation effectively alleviated these deficiencies and enhances the absorption of various minerals.</p></div>\",\"PeriodicalId\":7861,\"journal\":{\"name\":\"Animal Feed Science and Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-04-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0377840124000981/pdfft?md5=16146e3740a3faf9faa01929c7d721aa&pid=1-s2.0-S0377840124000981-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Feed Science and Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0377840124000981\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Feed Science and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0377840124000981","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Dietary copper levels affect mineral absorbability, rumen microbial composition and metabolites of the grazing Mongolian sheep
Copper (Cu) is a critical limiting factor in grazing livestock production in Inner Mongolia. This study investigated the effects of Cu-deficient and Cu-supplemented diets on production metrics, nutrient digestion and absorption, ruminal microbiota, and metabolites in grazing Mongolian sheep. A total of 28 individually-housed Mongolian sheep were randomly assigned to a Cu-deficient feeding group (LCu) and a Cu-deficient feeding control group (LCG), supplemented with a multi-nutrient salt containing 0 mg Cu and 14.3 mg Cu, respectively, for 53 continuous days. Following this, a 7-day digestion test was conducted, after which seven sheep from each group were randomly slaughtered. The remaining sheep in LCu and LCG transitioned to a Cu-supplemented feeding group (SCu) and a Cu-supplemented feeding control group (SCG), receiving multi-nutrient salts with 43.0 mg Cu and 14.3 mg Cu, respectively. This phase entailed a 34-day diet acclimatization period, a 7-day digestion test, and eventual slaughter. The LCu diet significantly decreased the ruminal Cu and sulfur (S) content, as well as Cu apparent absorbability, compared to the LCG group (P<0.05). Conversely, SCu significantly increased the ruminal Cu content and the apparent absorbability of phosphorus (P), S, potassium (K), calcium (Ca), manganese (Mn), iron (Fe), Cu and zinc (Zn) compared to the SCG group (P<0.05). Amplicon sequencing analysis revealed a significant downregulation of Alistipes and Barnesiella (P<0.05) in the LCu group. Metabolomic analyses supported an elevation in ruminal citrulline and L-ornithine levels, affecting amino acid metabolic pathways in the LCu group (P<0.05). In the SCu group, there was a significant increase in the relative abundance of Saccharofermentans and Ruminococcus (P<0.05), with metabolomic analysis revealed elevated levels of nucleic acid and purine analog affecting purine metabolic pathways (P<0.05). Notably, neither treatment had a significant impact production metrics, rumen fermentation parameters, or the apparent digestibility of crude protein, acid detergent fiber and neutral detergent fiber (P>0.05). In conclusion, a Cu-deficient diet impairs Cu absorption and nutrient catabolism in grazing Mongolian sheep, while Cu supplementation effectively alleviated these deficiencies and enhances the absorption of various minerals.
期刊介绍:
Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding.
Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome.
The journal covers the following areas:
Nutritive value of feeds (e.g., assessment, improvement)
Methods of conserving and processing feeds that affect their nutritional value
Agronomic and climatic factors influencing the nutritive value of feeds
Utilization of feeds and the improvement of such
Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins)
Mathematical models relating directly to animal-feed interactions
Analytical and experimental methods for feed evaluation
Environmental impacts of feed technologies in animal production.