Matheus Albino Souza , Mylena Lazareti Zanella , Gabriele Nichetti Vanin , Felipe Gomes Dallepiane , Camila Yasmin Monteiro Pizzi , Eduarda Rizzon Ferreira , Marciele Cristiane Spanenberg Fuhr , Nathan Mateus Piccolo , Huriel Scartazzini Palhano , Jordana da Silva Koch , Kellyn Rocca Souza , Ubirajara Maciel da Costa , Vanessa Valgas dos Santos , Liviu Steier , Charise Dallazem Bertol , José Antônio Poli de Figueiredo
{"title":"从创新电解装置中获得的次氯酸的抗菌作用和细胞毒性--体外研究","authors":"Matheus Albino Souza , Mylena Lazareti Zanella , Gabriele Nichetti Vanin , Felipe Gomes Dallepiane , Camila Yasmin Monteiro Pizzi , Eduarda Rizzon Ferreira , Marciele Cristiane Spanenberg Fuhr , Nathan Mateus Piccolo , Huriel Scartazzini Palhano , Jordana da Silva Koch , Kellyn Rocca Souza , Ubirajara Maciel da Costa , Vanessa Valgas dos Santos , Liviu Steier , Charise Dallazem Bertol , José Antônio Poli de Figueiredo","doi":"10.1016/j.archoralbio.2024.105966","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>This study evaluated the antimicrobial effect and cytotoxicity of hypochlorous acid(HClO) obtained from an innovative electrolytic device.</p></div><div><h3>Design</h3><p>The root canals of fifty extracted human teeth were inoculated with <em>Enterococcus faecalis</em> and divided into 5 groups (n = 10): DW (control); 2% chlorhexidine gel(CHX); 2.5% sodium hypochlorite(NaOCl); 250 ppm HClO and 500 ppm HClO. The counting of colony forming units evaluated the decontamination potential of each group. Cytotoxicity was evaluated after inoculation of tested protocols in fibroblastic cells for 3 min, calculating the cell viability. Specific statistical analysis was performed (α = 5%).</p></div><div><h3>Results</h3><p>The highest bacterial reduction was observed in experimental groups, with no statistical differences from each other (p > 0.05). The highest number of viable cells was observed in control group, followed by 250 ppm HClO and 500 ppm HClO groups, with statistical differences from each other (p < 0.05).</p></div><div><h3>Conclusions</h3><p>It could be concluded that HClO presented high antimicrobial activity and low cytotoxicity at both tested concentrations.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antimicrobial action and cytotoxicity of hypochlorous acid obtained from an innovative electrolytic device – An in vitro study\",\"authors\":\"Matheus Albino Souza , Mylena Lazareti Zanella , Gabriele Nichetti Vanin , Felipe Gomes Dallepiane , Camila Yasmin Monteiro Pizzi , Eduarda Rizzon Ferreira , Marciele Cristiane Spanenberg Fuhr , Nathan Mateus Piccolo , Huriel Scartazzini Palhano , Jordana da Silva Koch , Kellyn Rocca Souza , Ubirajara Maciel da Costa , Vanessa Valgas dos Santos , Liviu Steier , Charise Dallazem Bertol , José Antônio Poli de Figueiredo\",\"doi\":\"10.1016/j.archoralbio.2024.105966\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>This study evaluated the antimicrobial effect and cytotoxicity of hypochlorous acid(HClO) obtained from an innovative electrolytic device.</p></div><div><h3>Design</h3><p>The root canals of fifty extracted human teeth were inoculated with <em>Enterococcus faecalis</em> and divided into 5 groups (n = 10): DW (control); 2% chlorhexidine gel(CHX); 2.5% sodium hypochlorite(NaOCl); 250 ppm HClO and 500 ppm HClO. The counting of colony forming units evaluated the decontamination potential of each group. Cytotoxicity was evaluated after inoculation of tested protocols in fibroblastic cells for 3 min, calculating the cell viability. Specific statistical analysis was performed (α = 5%).</p></div><div><h3>Results</h3><p>The highest bacterial reduction was observed in experimental groups, with no statistical differences from each other (p > 0.05). The highest number of viable cells was observed in control group, followed by 250 ppm HClO and 500 ppm HClO groups, with statistical differences from each other (p < 0.05).</p></div><div><h3>Conclusions</h3><p>It could be concluded that HClO presented high antimicrobial activity and low cytotoxicity at both tested concentrations.</p></div>\",\"PeriodicalId\":8288,\"journal\":{\"name\":\"Archives of oral biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-04-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of oral biology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0003996924000876\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of oral biology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0003996924000876","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Antimicrobial action and cytotoxicity of hypochlorous acid obtained from an innovative electrolytic device – An in vitro study
Objective
This study evaluated the antimicrobial effect and cytotoxicity of hypochlorous acid(HClO) obtained from an innovative electrolytic device.
Design
The root canals of fifty extracted human teeth were inoculated with Enterococcus faecalis and divided into 5 groups (n = 10): DW (control); 2% chlorhexidine gel(CHX); 2.5% sodium hypochlorite(NaOCl); 250 ppm HClO and 500 ppm HClO. The counting of colony forming units evaluated the decontamination potential of each group. Cytotoxicity was evaluated after inoculation of tested protocols in fibroblastic cells for 3 min, calculating the cell viability. Specific statistical analysis was performed (α = 5%).
Results
The highest bacterial reduction was observed in experimental groups, with no statistical differences from each other (p > 0.05). The highest number of viable cells was observed in control group, followed by 250 ppm HClO and 500 ppm HClO groups, with statistical differences from each other (p < 0.05).
Conclusions
It could be concluded that HClO presented high antimicrobial activity and low cytotoxicity at both tested concentrations.
期刊介绍：
Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including:
Cell and molecular biology
Molecular genetics
Immunology
Pathogenesis
Cellular microbiology
Embryology
Syndromology
Forensic dentistry
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