{"title":"石墨、氧化石墨烯和多壁碳纳米管对组织工程应用中壳聚糖/透明质酸/羟基磷灰石支架的理化特性和生物相容性的影响","authors":"Siti Fauziyah Rahman , Muhammad Maulana Ghiffary , Joshua Yoshihiko Tampubolon , Elly Septia Yulianti , Muhammad Hanif Nadhif , Puspita Anggraini Katili , Siti Hanafiah , Azizah Intan Pangesty , Muhammad Artha Jabatsudewa Maras","doi":"10.1016/j.jsamd.2024.100719","DOIUrl":null,"url":null,"abstract":"<div><p>Bone tissue engineering (BTE) is a promising alternative approach to the repair of damaged bone tissue. This study aims to fabricate and characterize scaffolds composed of chitosan (CS), hyaluronic acid (HA), hydroxyapatite (HAp), and a combination of graphite (Gr), graphene oxide (GO), and multi-walled carbon nanotubes (MWCNT) for BTE applications. The Gr and MWCNT were functionalized by acid oxidation, while the GO was synthesized using the improved Hummers' method. The scaffolds were prepared by lyophilization, and the physical, chemical, and biological properties were evaluated. Scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy (EDS), Fourier transform infrared (FTIR) spectroscopy, mechanical testing, water contact angle, degradation, and biocompatibility assays were used to characterize the scaffolds. The degradation rate was determined using the liquid displacement method. Pores of different sizes were present on the surface of and throughout the scaffold. According to the FTIR results, the scaffolds contained functional groups that promote cell differentiation and proliferation. These scaffolds have compressive strength, Young's modulus, and toughness similar to cancellous bone, with reasonable porosity and controllable degradation rates. Biocompatibility testing confirmed that the scaffolds support cell proliferation and differentiation.</p></div>","PeriodicalId":17219,"journal":{"name":"Journal of Science: Advanced Materials and Devices","volume":"9 2","pages":"Article 100719"},"PeriodicalIF":6.7000,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468217924000509/pdfft?md5=1812e4832325d224c6249e98536f7333&pid=1-s2.0-S2468217924000509-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Effect of graphite, graphene oxide, and multi-walled carbon nanotubes on the physicochemical characteristics and biocompatibility of chitosan/hyaluronic acid/hydroxyapatite scaffolds for tissue engineering applications\",\"authors\":\"Siti Fauziyah Rahman , Muhammad Maulana Ghiffary , Joshua Yoshihiko Tampubolon , Elly Septia Yulianti , Muhammad Hanif Nadhif , Puspita Anggraini Katili , Siti Hanafiah , Azizah Intan Pangesty , Muhammad Artha Jabatsudewa Maras\",\"doi\":\"10.1016/j.jsamd.2024.100719\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Bone tissue engineering (BTE) is a promising alternative approach to the repair of damaged bone tissue. 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引用次数: 0
摘要
骨组织工程(BTE)是修复受损骨组织的一种前景广阔的替代方法。本研究旨在制备和表征由壳聚糖(CS)、透明质酸(HA)、羟基磷灰石(HAp)以及石墨(Gr)、氧化石墨烯(GO)和多壁碳纳米管(MWCNT)组合而成的支架,以用于 BTE 应用。石墨(Gr)和多壁碳纳米管(MWCNT)是通过酸氧化法进行功能化的,而氧化石墨烯(GO)则是采用改进的 Hummers 方法合成的。通过冻干法制备了支架,并对其物理、化学和生物特性进行了评估。使用扫描电子显微镜(SEM)、能量色散 X 射线光谱(EDS)、傅立叶变换红外光谱(FTIR)、机械测试、水接触角、降解和生物相容性检测来表征支架。降解率采用液体置换法测定。支架表面和整个支架都存在不同大小的孔隙。傅立叶变换红外光谱结果表明,支架含有促进细胞分化和增殖的官能团。这些支架具有与松质骨相似的抗压强度、杨氏模量和韧性,孔隙率合理,降解速率可控。生物相容性测试证实,这些支架支持细胞增殖和分化。
Effect of graphite, graphene oxide, and multi-walled carbon nanotubes on the physicochemical characteristics and biocompatibility of chitosan/hyaluronic acid/hydroxyapatite scaffolds for tissue engineering applications
Bone tissue engineering (BTE) is a promising alternative approach to the repair of damaged bone tissue. This study aims to fabricate and characterize scaffolds composed of chitosan (CS), hyaluronic acid (HA), hydroxyapatite (HAp), and a combination of graphite (Gr), graphene oxide (GO), and multi-walled carbon nanotubes (MWCNT) for BTE applications. The Gr and MWCNT were functionalized by acid oxidation, while the GO was synthesized using the improved Hummers' method. The scaffolds were prepared by lyophilization, and the physical, chemical, and biological properties were evaluated. Scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy (EDS), Fourier transform infrared (FTIR) spectroscopy, mechanical testing, water contact angle, degradation, and biocompatibility assays were used to characterize the scaffolds. The degradation rate was determined using the liquid displacement method. Pores of different sizes were present on the surface of and throughout the scaffold. According to the FTIR results, the scaffolds contained functional groups that promote cell differentiation and proliferation. These scaffolds have compressive strength, Young's modulus, and toughness similar to cancellous bone, with reasonable porosity and controllable degradation rates. Biocompatibility testing confirmed that the scaffolds support cell proliferation and differentiation.
期刊介绍:
In 1985, the Journal of Science was founded as a platform for publishing national and international research papers across various disciplines, including natural sciences, technology, social sciences, and humanities. Over the years, the journal has experienced remarkable growth in terms of quality, size, and scope. Today, it encompasses a diverse range of publications dedicated to academic research.
Considering the rapid expansion of materials science, we are pleased to introduce the Journal of Science: Advanced Materials and Devices. This new addition to our journal series offers researchers an exciting opportunity to publish their work on all aspects of materials science and technology within the esteemed Journal of Science.
With this development, we aim to revolutionize the way research in materials science is expressed and organized, further strengthening our commitment to promoting outstanding research across various scientific and technological fields.