分布在阿塞拜疆的蒿属(菊科)物种的遗传结构和分子分析

Narmin Sadıgova, Z. Suleymanova, J. Ojaghi, Sayyara İbadullayeva, A. Mammadov
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引用次数: 0

摘要

本研究使用 RAPD 引物研究了从阿塞拜疆不同地区采集的 10 个沙蒿(Artemisia szowitziana (Besser) Grossh)和香蒿(Artemisia fragrans Willd.)物种基因型的系统发育关系和遗传结构。使用选定的 RAPD 引物共扩增出 94 个扩增子。其中,用 OPA-02 引物扩增出 3 条带,用 OPW-17 引物扩增出 12 条带。在所研究的 RAPD 标记中,OPW-17、OPT-19 和 OPT-20 引物的多态性最高,分别为 100、90.91 和 90%。然而,根据 10 个不同的 RAPD 标记计算得出的多态性平均值高达 72.79%。多态性信息含量(PIC)的平均值(0.864)表明所研究样本具有丰富的遗传多样性。利用 Jaccard 相似度指数和 UPGMA 方法进行的聚类分析将所有青蒿样品分为 6 大类。主成分分析(PCA)证明了总方差的 74.22%。根据所获得的结果,可以看出阿塞拜疆的 szowitziana 蒿(1-2 和 4-6 个样本)和 fragrans 蒿(3、5、7 和 8、9、10 个样本)种群之间以及蒿属物种种群之间在分子水平上存在广泛的多样性。
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Genetic structure and molecular analysis of the species of the genus Artemisia L. (Asteraceae) distributed in Azerbaijan
In this study, the phylogenetic relationships and genetic structure of 10 collected genotypes of Artemisia szowitziana (Besser) Grossh and Artemisia fragrans Willd. species from different regions of Azerbaijan were investigated using RAPD primers. A total of 94 amplicons were amplified using selected RAPD primers. Among them 3 band with OPA-02 primers and 12 band with OPW-17 primers were amplified. The highest polymorphism among the investigated RAPD markers was 100, 90.91 and 90%, which has been obtained by OPW-17, OPT-19, and OPT-20 primers, respectively. However, the calculated average value of polymorphism was as high as 72.79% based on 10 different RAPD markers. The average PIC (Polymorphism Information Content) value (0.864) has indicated the rich genetic diversity among the studied samples. The cluster analyses by using Jaccard similarity index and UPGMA method classified all Artemisia L. samples into 6 major groups. Principal Component Analysis (PCA) justified 74.22% of the total variance. Based on the results obtained, it has been observed that there is a wide range of diversity in the molecular level between the population of Artemisia szowitziana (1-2 and 4-6 samples) and Artemisia fragrans (3, 5, 7 and 8, 9, 10 samples) and between population of the Artemisia species in Azerbaijan.
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来源期刊
International Journal of Secondary Metabolite
International Journal of Secondary Metabolite Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
1.40
自引率
0.00%
发文量
28
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