INCREASING GROWTH RATE OF Botryocuccus braunii USING ULTRASONIC WAVES

Microalgae have emerged as a promising alternative biomass source, offering distinct advantages over conventional crops in terms of oil content per mass. Maximizing their reproductive capacity throughout their life cycle is essential for optimizing biomass production. This study investigates the influence of ultrasonic waves on the reproductive rate of Botryococcus braunii microalgae. Various ultrasonic parameters, including power (56.2, 86.3, and 132.2 W for 37 kHz; 46.3, 67.2, and 101.2 W for 80 kHz), exposure times (3 and 5 min), and time intervals (2 and 4 days) were examined. The best outcomes were obtained with a 37 kHz ultrasonic frequency, 83.6 W power, 3-min exposure, and a 2-day interval, resulting in a maximum relative specific growth rate (16.67%) and relative biomass productivity (34.62%). Beyond these parameters, adjustments in ultrasonic treatment conditions resulted in decreased growth rates and biomass productivity. It is crucial to consider multiple ultrasonic factors, including frequency, power, exposure time, and time intervals, as each parameter significantly impacts microalgae growth.