黑兵蝇(BSF)幼虫肠道分离物中的细菌和毛霉对卷心辣椒(Capsicum annuum)抗病性的影响

Melinda Kristi, Y. Sanjaya, K. Kusnadi
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摘要

辣椒(卷心菜红辣椒)是社区的重要商品。农民使用化学农药来提高植物的抗病能力。使用化学农药对环境有害,长期食用会产生影响。因此,需要创新生物农药。黑兵蝇(BSF)是一种肠道内有细菌和真菌的昆虫。在 BSF 肠道分离物中发现的细菌和真菌可作为植物的抗菌剂。因此,本研究调查了细菌和真菌病毒毛霉对提高 C. annuum 植物抗病性的影响。本研究共有 4 种处理方法,即使用化学杀菌剂、BSF 细菌配方、T. viride.BSF,以及细菌和 T. viride BSF 的混合物。研究结果通过计算侵染强度(IS)得分、实验室显微观察和统计检验进行分析。对照植株的 IS 评分为 75.0%,杀真菌剂处理为 37.5%,单独细菌处理为 33.3%,病毒唑处理为 19.4%,细菌和病毒唑混合处理为 4.2%。所有植株都受到 Cercospora sp.叶斑病的侵害,对照植株受到 Sclerotium rolfsii 真菌枯萎病和 Leveillula taurica 白粉病以及黄疸病的侵害。黄疸病也侵袭了只用病毒菌处理和只用细菌处理的植株。正态性和同质性检验表明,数据不正常也不均匀,但根据 Kruskal Wallis 检验,H1 是可以接受的。本研究的结论是,BSF 中的细菌和 T. viride 能够提高卷心辣椒植株的抗病性,但在预防由 Cercospora sp.
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Pengaruh Pemberian Bakteri dan Trichoderma viride dari Isolat Usus Larva Black Soldier Fly (BSF) terhadap Ketahan Penyakit Cabai Keriting (Capsicum annuum)
Capsicum annuum (curly red chili) is an important commodity for the community. The addition of chemical pesticides is used by farmers to increase plant disease resistance. The use of chemical pesticides is harmful to the environment and can have long-term effects if consumed. Therefore, biopesticide innovation is needed. Black Soldier Fly (BSF) is an insect that has bacteria and fungi in its gut. Bacteria and fungi found in BSF intestinal isolates can function as antimicrobials for plants. Because of this, in this study investigated the effect of the bacteria and fungi Trichoderma viride in increasing disease resistance in C. annuum plants. There were 4 treatments in this study, namely the administration of chemical fungicides, BSF bacterial formula, T. viride. BSF, and a mixture of bacteria and T. viride BSF. The results obtained were analyzed by calculating the Intensity of Attack (IS) score, microscopic observations in the laboratory, and statistical tests. The IS score of the control plants was 75.0%, the fungicide treatment was 37.5%, the bacterial treatment alone was 33.3%, the T. viride treatment was 19.4%, and the mixed treatment of bacteria and T. viride was 4.2%. All plants were attacked by leaf spot disease by Cercospora sp. Control plants were attacked by Sclerotium rolfsii fungus wilt and powdery mildew by Leveillula taurica and jaundice. Jaundice also attacked plants treated with T. viride only and bacteria only. The normality and homogeneity tests show that the data is not normal and not homogeneous but H1 is acceptable based on the Kruskal Wallis test. The conclusion of this study is that bacteria and T. viride from BSF are able to increase disease resistance in curly chili plants, but are not yet effective in preventing disease caused by Cercospora sp.
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