班佑万吉传统市场鸡肉中的微生物污染

Prima Ayu Wibawati, Yunia Chrismonica, Racca Biana Susanti, Mario Putra Himawan, Afaf Heny Sintya Devy, Juwita Ayu Nirmalasari, Abzal Abdramanov
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摘要

本研究旨在分析东爪哇省班佑万吉(Banyuwangi)传统市场鸡肉的微生物污染及其 pH 值,以确保公众健康。本研究共使用了来自三个传统市场的 30 份生鲜鸡肉样本,并对样本中是否存在大肠杆菌和沙门氏菌进行了检测。 使用革兰氏染色法、培养法和生化检测法对大肠杆菌和沙门氏菌进行了鉴定。对大肠菌群和大肠埃希氏菌采用最大可能数(MPN)法。在购买鸡肉后和冷藏 24 小时后,测量鸡肉的 pH 值。结果显示,所有样本中的大肠菌群平均超过 1100 CFU/G,大肠杆菌污染率为 20%(6/30),超过了建议的最高限值。此外,所有样本中沙门氏菌属的鉴定结果均为阴性。鸡肉的 pH 值随宰杀时间的延长而降低;购买后(0 小时),pH 值为 5.78 ± 0.26,存放 24 小时后,pH 值升至 6.02 ± 0.28。总之,从班佑万吉一些传统市场采集的 30 个生鲜鸡肉样本中,大肠菌群污染严重。此外,大肠杆菌污染率高达 20%,沙门氏菌属呈阴性。
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Microbial Contamination in Chicken Meat in Traditional Markets in Banyuwangi
This study aimed to analyze the microbial contamination of chicken meat and its pH value from traditional markets in Banyuwangi, East Java to ensure public health. A total of 30 raw chicken meat samples from three traditional markets were used in this study and examined for the presence of Escherichia coli and Salmonella spp. E. coli and Salmonella spp. were identified using Gram staining, culturing, and biochemical tests. The Most Probable Number (MPN) method was used for coliform and E. coli. Measuring the pH value of chicken meat was carried out after purchasing the meat and 24 hours after storage at cold temperatures. The results showed coliform contamination in all samples with an average of >1100 CFU/G with E. coli contamination of 20% (6/30), exceeding the maximum recommended limit. Furthermore, the identification of Salmonella spp. in all samples showed negative results. Chicken meat pH value decreased with the length of postmortem time; immediately after purchase (0 hours), the pH value was 5.78 ± 0.26, and after 24 hours of storage, it raised 6.02 ± 0.28. In conclusion, there was high coliform contamination out of 30 raw chicken meat samples taken from some traditional markets in Banyuwangi. Furthermore, E. coli contamination was as much as 20%, and Salmonella spp. was negative.
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