尾矿池细菌分离物的特征及其对重金属和抗生素的耐药性

IF 1 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2024-04-21 DOI:10.1134/S0003683824020157
I. D. Radojević, K. G. Ćirković, M. Ž. Grujović, K. G. Mladenović, A. M. Ostojić
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摘要

l 水样采集自鲁德尼克山区的一个尾矿池废水处理场,那里的多金属矿浮选过程会产生高浓度的 Pb2+、Zn2+ 和 Cu2+。这项研究旨在确定对重金属具有抗性的微生物,并评估其进行生物修复的潜力。使用分光光度计分析了微生物在温度、pH 值和 NaCl 浓度等不同条件下的生长能力。测定了浮游细胞及其生物膜对测试物质的最小抑制浓度和致死浓度。主要分离菌株,即高度芽孢杆菌 PMFKG-R3、普米氏芽孢杆菌 PMFKG-R15、蜡样芽孢杆菌 PMFKG-R46、疣状假单胞菌 PMFKG-R30 和聚合泛德菌 PMFKG-R20,在 22 和 37 摄氏度的环境中均有生长能力,并表现出耐盐性,但对酸性 pH 值敏感。大多数浮游生物和生物膜形式的分离物对重金属,特别是 Pb2+ 和 Zn2+ 有明显的抗性,这与采样地点相符。值得注意的是,浮游细胞对抗生素敏感,而生物膜则表现出稍高的抗药性。P. veronii PMFKG-R30 和 P. agglomerans PMFKG-R20 显示出对重金属的抗性和对抗生素的敏感性,有望用于生物修复目的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Characterization of Bacterial Isolates from Tailings Pond and Their Resistance to Heavy Metals and Antibiotics

lWater samples were collected from a tailings pond wastewater site in the Rudnik mountain area, where polymetallic ore flotation processes generate high concentrations of Pb2+, Zn2+, and Cu2+. The study aimed to identify microorganisms resistant to heavy metals and assess their potential for bioremediation. Growth capabilities under varying conditions, including temperature, pH, and NaCl concentrations, were analyzed using a spectrophotometer. Minimal inhibitory and lethal concentrations of tested substances were determined for both planktonic cells and their biofilms. Key isolates, namely Bacillus altitudinis PMFKG-R3, B. pumilus PMFKG-R15, B. cereus PMFKG-R46, Pseudomonas veronii PMFKG-R30, and Pantoea agglomerans PMFKG-R20, demonstrated growth ability at both 22 and 37°C and exhibited halotolerance, albeit sensitivity to acidic pH. Most isolates in both planktonic and biofilm forms displayed notable resistance to heavy metals, particularly Pb2+ and Zn2+, in line with the sampling location. Notably, planktonic cells were sensitive to antibiotics, while biofilms exhibited slightly higher resistance. Promising candidates for bioremediation purposes were identified in P. veronii PMFKG-R30 and P. agglomerans PMFKG-R20, which displayed resistance to heavy metals and sensitivity to antibiotics.

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来源期刊
Applied Biochemistry and Microbiology
Applied Biochemistry and Microbiology 生物-生物工程与应用微生物
CiteScore
1.70
自引率
12.50%
发文量
75
审稿时长
6-12 weeks
期刊介绍: Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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