Assessment of the Effectiveness of Revascularization Methods Utilizing Platelet-Rich Fibrin (PRF) Concentrate Versus Clot formation as a Supportive Matrix

Introduction: Revascularization therapy for young permanent teeth with apical pathology has gained widespread acceptance as an alternative to traditional apexification procedures. In the context of regenerative endodontic treatment (RET), both PRF and clot formation [1] have been used as scaffolds. Objective : This study aimed to evaluate the microscopic tissue-level outcomes of the revascularization process in young permanent premolar dog teeth in vitro. This experimental study aimed to evaluate histological outcomes of the process in immature dog teeth. Methods : Sixty roots that were not fully developed were divided into three experimental groups (control and study) and each group divided again into subgroups (positive and negative) based on the treatment protocol. Moreover, samples from the normal pulp of dog teeth were incorporated. Results : The study group showed a statistically significant increase the percentage of blood vessels and odontoblasts numbers in comparison to the other two groups (P value < 0.001, P=0.003). In terms of inflammatory count, the negative control group showed a higher count in comparison the other two groups Conclusions : Clot formation and Platelet-Rich Plasma (PRP) demonstrated comparable outcomes in periapical healing, specifically regarding mean area fraction of blood vessels and odontoblasts numbers.