屠宰场中牛的大肠埃希菌多重耐药性鉴定

Yolla Rona Mustika, M. Effendi, Yulianna Puspitasari, H. Plumeriastuti, A. Khairullah, Kurnia Nisa Kinasih
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引用次数: 0

摘要

大肠杆菌是动物消化道的典型菌群。基于这些细节,本研究对泗水屠宰场中牛的耐多药大肠杆菌进行了检测和鉴定。每份牛直肠拭子样本都在 EMB 琼脂培养基中进行了菌落培养,然后进行了革兰氏染色。使用 IMViC 检验确认大肠杆菌的存在。根据形态培养特征、革兰氏染色和生化检测,样本检查结果显示,在 100 份牛直肠拭子样本中,有 41 份样本(41%)被确认为大肠杆菌阳性。大肠杆菌对抗生素的耐药性测试结果显示,在总共 41 个大肠杆菌分离物中,12 个分离物(29.27%)被检测出对 1 类抗生素具有耐药性,2 个分离物(4.88%)对 2 类抗生素具有耐药性,3 个分离物(7.32%)被证实对多种药物具有耐药性(MDR)。
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Identification of Escherichia coli Multidrug Resistance in Cattle in Abattoirs
Escherichia coli is a typical flora of an animals' digestive tract. Based on these details, this study was done on the detection and identification of multidrug-resistant E. coli in cattle in Surabaya abattoirs. Each cattle rectum swab sample was streaked into EMB agar media and followed by Gram staining. The IMViC test was used to confirm the presence of E. coli. Based on morphological culture features, Gram staining, and biochemical testing, the sample examination results revealed that 41 samples (41%) of the 100 cattle rectal swab samples that were isolated were confirmed to be positive for E. coli. The profile of antibiotic resistance from the results of the E. coli resistance test to antibiotics showed that out of a total of 41 isolates of E. coli, 12 isolates (29.27%) were detected as resistant to 1 class of antibiotics tested, while 2 isolates (4.88%) were resistant to 2 classes of antibiotics, and 3 isolates (7.32%) were confirmed to be multidrug resistance (MDR).
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