双定位过氧化氢酶的复杂平衡调节了西摩里钩端螺旋体(Kinetoplastea: Trypanosomatidae)的感染性。

IF 3.7 2区 医学 Q1 PARASITOLOGY International journal for parasitology Pub Date : 2024-04-23 DOI:10.1016/j.ijpara.2024.04.007
Ľubomíra Chmelová , Natalya Kraeva , Andreu Saura , Adam Krayzel , Cecilia Stahl Vieira , Tainá Neves Ferreira , Rodrigo Pedro Soares , Barbora Bučková , Arnau Galan , Eva Horáková , Barbora Vojtková , Jovana Sádlová , Marina N. Malysheva , Anzhelika Butenko , Galina Prokopchuk , Alexander O. Frolov , Julius Lukeš , Anton Horváth , Ingrid Škodová-Sveráková , Denise Feder , Vyacheslav Yurchenko
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引用次数: 0

摘要

几乎所有需氧生物都具有过氧化氢酶,这是一种清除过氧化氢的强力酶,有助于抵御有害的活性氧。在锥虫的共同祖先中并不存在这种酶,但单细胞锥虫的不同品系至少三次从不同细菌中独立获得了这种酶。这一观察结果提出了一个显而易见的问题:既然许多锥虫类群在没有过氧化氢酶的情况下也能正常工作,为什么过氧化氢酶会如此 "抢手 "呢?在这项工作中,我们分析了过氧化氢酶在赛毛氏钩端螺旋体中的亚细胞定位和功能。我们证明,这种酶存在于细胞质和糖体的一个子集中,而且它在细胞质中的保留是依赖于 H2O2 的。这种寄生虫体内过氧化氢酶的消减不会对其造成危害,而过量表达过氧化氢酶则会在秘鲁痢疾杆菌的实验感染中导致寄生虫数量大幅增加。我们认为,所研究的鞭毛虫能够调节昆虫宿主中肠中过氧化氢酶的活性,这有利于它们的发育,并保护它们在高温下免受氧化损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Intricate balance of dually-localized catalase modulates infectivity of Leptomonas seymouri (Kinetoplastea: Trypanosomatidae)

Nearly all aerobic organisms are equipped with catalases, powerful enzymes scavenging hydrogen peroxide and facilitating defense against harmful reactive oxygen species. In trypanosomatids, this enzyme was not present in the common ancestor, yet it had been independently acquired by different lineages of monoxenous trypanosomatids from different bacteria at least three times. This observation posited an obvious question: why was catalase so “sought after” if many trypanosomatid groups do just fine without it? In this work, we analyzed subcellular localization and function of catalase in Leptomonas seymouri. We demonstrated that this enzyme is present in the cytoplasm and a subset of glycosomes, and that its cytoplasmic retention is H2O2-dependent. The ablation of catalase in this parasite is not detrimental in vivo, while its overexpression resulted in a substantially higher parasite load in the experimental infection of Dysdercus peruvianus. We propose that the capacity of studied flagellates to modulate the catalase activity in the midgut of its insect host facilitates their development and protects them from oxidative damage at elevated temperatures.

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来源期刊
CiteScore
8.40
自引率
2.50%
发文量
76
审稿时长
23 days
期刊介绍: International Journal for Parasitology offers authors the option to sponsor nonsubscriber access to their articles on Elsevier electronic publishing platforms. For more information please view our Sponsored Articles page. The International Journal for Parasitology publishes the results of original research in all aspects of basic and applied parasitology, including all the fields covered by its Specialist Editors, and ranging from parasites and host-parasite relationships of intrinsic biological interest to those of social and economic importance in human and veterinary medicine and agriculture.
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