干扰 RGD1 基因可提高酿酒酵母对醋酸的耐受性

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2024-04-21 DOI:10.1134/S000368382402008X

H. S. Kim

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引用次数: 0

摘要

本研究旨在以酵母为合适的真核生物模型，鉴定与醋酸耐受性相关的基因，并阐明其耐受机制。本研究通过筛选转座子介导的突变体文库，分离出一株耐受醋酸胁迫的菌株，并确定其破坏的基因为 RGD1。此外，通过删除和过表达该相应基因，也证实了这种与醋酸耐受性相关的表型。该突变株在应对醋酸胁迫时表现出 Hog1p 的激活以及 MSN2/4 和 STRE 介导基因（如 CTT1 和 HSP12）的表达增加。

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Disruption of RGD1 Gene Improves Acetic Acid Tolerance in Saccharomyces cerevisiae

This study aimed to identify genes related to acetic acid tolerance and elucidate the tolerance mechanism in yeast Saccharomyces cerevisiae as an appropriate eukaryote model. In this study, one tolerant strain exposed to acetic acid stress was isolated by screening a transposon-mediated mutant library and the disrupted gene was identified to be RGD1. In addition, this phenotype related to acetic acid tolerance was confirmed by deletion and overexpressing of this corresponding gene. This mutant strain showed activation of Hog1p and increased expression of the MSN2/4 and STRE-mediated genes such as CTT1 and HSP12 in response to acetic acid stress.

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.