长期饮酒会改变猕猴的性别依赖性 BNST 神经元功能

IF 4.3 2区 医学 Q1 NEUROSCIENCES Neurobiology of Stress Pub Date : 2024-04-30 DOI:10.1016/j.ynstr.2024.100638
Kristen E. Pleil , Kathleen A. Grant , Verginia C. Cuzon Carlson , Thomas L. Kash
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引用次数: 0

摘要

反复饮酒会导致多种神经精神疾病,包括酒精使用障碍以及共同表现的焦虑和情绪障碍。在与男性相同的酒精接触史下,女性更容易患上和表现出这些疾病,这表明她们可能对控制饮酒、压力反应和奖赏处理等行为的边缘脑区的酒精诱导可塑性更敏感。我们利用恒河猴饮酒转化模型,研究了纹状体末端床核(BNST)神经元的基础功能和可塑性的性别差异。我们对每天 "开放"(22 小时/天)接触 4% 乙醇和水超过一年的雄性和雌性猴子或对照组的 BNST 神经元进行了切片电生理学记录。我们发现,与对照组雄猴的神经元相比,对照组雌猴的 BNST 神经元的总体电流密度、超极化激活去极化电流(Ih)和内向整流都有所降低,膜电阻也更高,突触谷氨酸能释放和兴奋驱动力也更强,这表明雌猴的 BNST 神经元比雄猴的神经元基础兴奋性更高。长期饮酒会使男女神经元的这些指标发生变化,降低电流密度、Ih和内向整流,增加突触兴奋。此外,雄性 BNST 神经元的网络活动依赖性突触抑制在基础上高于雌性,而酒精暴露会增加这种抑制,这是一种抵消过度兴奋的假定同调机制。总之,这些结果表明,雌性恒河猴 BNST 的基础兴奋性高于雄性,而长期饮酒会导致兴奋性和突触兴奋的总体增加。这些结果揭示了导致雌性易患神经精神疾病(包括共同表现的焦虑症和酒精使用障碍)的机制。
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Chronic alcohol consumption alters sex-dependent BNST neuron function in rhesus macaques

Repeated alcohol drinking contributes to a number of neuropsychiatric diseases, including alcohol use disorder and co-expressed anxiety and mood disorders. Women are more susceptible to the development and expression of these diseases with the same history of alcohol exposure as men, suggesting they may be more sensitive to alcohol-induced plasticity in limbic brain regions controlling alcohol drinking, stress responsivity, and reward processing, among other behaviors. Using a translational model of alcohol drinking in rhesus monkeys, we examined sex differences in the basal function and plasticity of neurons in the bed nucleus of the stria terminalis (BNST), a brain region in the extended amygdala shown to be a hub circuit node dysregulated in individuals with anxiety and alcohol use disorder. We performed slice electrophysiology recordings from BNST neurons in male and female monkeys following daily “open access” (22 h/day) to 4% ethanol and water for more than one year or control conditions. We found that BNST neurons from control females had reduced overall current density, hyperpolarization-activated depolarizing current (Ih), and inward rectification, as well as higher membrane resistance and greater synaptic glutamatergic release and excitatory drive, than those from control males, suggesting that female BNST neurons are more basally excited than those from males. Chronic alcohol drinking produced a shift in these measures in both sexes, decreasing current density, Ih, and inward rectification and increasing synaptic excitation. In addition, network activity-dependent synaptic inhibition was basally higher in BNST neurons of males than females, and alcohol exposure increased this in both sexes, a putative homeostatic mechanism to counter hyperexcitability. Altogether, these results suggest that the rhesus BNST is more basally excited in females than males and chronic alcohol drinking produces an overall increase in excitability and synaptic excitation. These results shed light on the mechanisms contributing to the female-biased susceptibility to neuropsychiatric diseases including co-expressed anxiety and alcohol use disorder.

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来源期刊
Neurobiology of Stress
Neurobiology of Stress Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
9.40
自引率
4.00%
发文量
74
审稿时长
48 days
期刊介绍: Neurobiology of Stress is a multidisciplinary journal for the publication of original research and review articles on basic, translational and clinical research into stress and related disorders. It will focus on the impact of stress on the brain from cellular to behavioral functions and stress-related neuropsychiatric disorders (such as depression, trauma and anxiety). The translation of basic research findings into real-world applications will be a key aim of the journal. Basic, translational and clinical research on the following topics as they relate to stress will be covered: Molecular substrates and cell signaling, Genetics and epigenetics, Stress circuitry, Structural and physiological plasticity, Developmental Aspects, Laboratory models of stress, Neuroinflammation and pathology, Memory and Cognition, Motivational Processes, Fear and Anxiety, Stress-related neuropsychiatric disorders (including depression, PTSD, substance abuse), Neuropsychopharmacology.
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