Yujie Wang, Zhengao Di*, Minglang Qin, Shenming Qu, Wenbo Zhong, Lingfeng Yuan, Jing Zhang, Julian M. Hibberd and Ziyi Yu*,
{"title":"通过烟草 BY-2 细胞在定制颗粒水凝胶支架中的生长和转染,推进工程植物活体材料的发展","authors":"Yujie Wang, Zhengao Di*, Minglang Qin, Shenming Qu, Wenbo Zhong, Lingfeng Yuan, Jing Zhang, Julian M. Hibberd and Ziyi Yu*, ","doi":"10.1021/acscentsci.4c00338","DOIUrl":null,"url":null,"abstract":"<p >In this study, an innovative approach is presented in the field of engineered plant living materials (EPLMs), leveraging a sophisticated interplay between synthetic biology and engineering. We detail a 3D bioprinting technique for the precise spatial patterning and genetic transformation of the tobacco BY-2 cell line within custom-engineered granular hydrogel scaffolds. Our methodology involves the integration of biocompatible hydrogel microparticles (HMPs) primed for 3D bioprinting with <i>Agrobacterium tumefaciens</i> capable of plant cell transfection, serving as the backbone for the simultaneous growth and transformation of tobacco BY-2 cells. This system facilitates the concurrent growth and genetic modification of tobacco BY-2 cells within our specially designed scaffolds. These scaffolds enable the cells to develop into predefined patterns while remaining conducive to the uptake of exogenous DNA. We showcase the versatility of this technology by fabricating EPLMs with unique structural and functional properties, exemplified by EPLMs exhibiting distinct pigmentation patterns. These patterns are achieved through the integration of the betalain biosynthetic pathway into tobacco BY-2 cells. Overall, our study represents a groundbreaking shift in the convergence of materials science and plant synthetic biology, offering promising avenues for the evolution of sustainable, adaptive, and responsive living material systems.</p><p >Utilizing <i>Nicotiana tabacum</i> BY-2 cells within custom hydrogel scaffolds, this research demonstrates the creation of genetically modified engineered plant living materials with specific geometries and functionalities. Innovative granular hydrogel microparticles facilitate cell growth and DNA transformation, leading to those materials with unique pigmentation and fluorescent patterns. This advancement opens new avenues in bioengineering, offering diverse applications in bioengineering and material science.</p>","PeriodicalId":10,"journal":{"name":"ACS Central Science","volume":null,"pages":null},"PeriodicalIF":12.7000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acscentsci.4c00338","citationCount":"0","resultStr":"{\"title\":\"Advancing Engineered Plant Living Materials through Tobacco BY-2 Cell Growth and Transfection within Tailored Granular Hydrogel Scaffolds\",\"authors\":\"Yujie Wang, Zhengao Di*, Minglang Qin, Shenming Qu, Wenbo Zhong, Lingfeng Yuan, Jing Zhang, Julian M. Hibberd and Ziyi Yu*, \",\"doi\":\"10.1021/acscentsci.4c00338\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >In this study, an innovative approach is presented in the field of engineered plant living materials (EPLMs), leveraging a sophisticated interplay between synthetic biology and engineering. We detail a 3D bioprinting technique for the precise spatial patterning and genetic transformation of the tobacco BY-2 cell line within custom-engineered granular hydrogel scaffolds. Our methodology involves the integration of biocompatible hydrogel microparticles (HMPs) primed for 3D bioprinting with <i>Agrobacterium tumefaciens</i> capable of plant cell transfection, serving as the backbone for the simultaneous growth and transformation of tobacco BY-2 cells. This system facilitates the concurrent growth and genetic modification of tobacco BY-2 cells within our specially designed scaffolds. These scaffolds enable the cells to develop into predefined patterns while remaining conducive to the uptake of exogenous DNA. We showcase the versatility of this technology by fabricating EPLMs with unique structural and functional properties, exemplified by EPLMs exhibiting distinct pigmentation patterns. These patterns are achieved through the integration of the betalain biosynthetic pathway into tobacco BY-2 cells. Overall, our study represents a groundbreaking shift in the convergence of materials science and plant synthetic biology, offering promising avenues for the evolution of sustainable, adaptive, and responsive living material systems.</p><p >Utilizing <i>Nicotiana tabacum</i> BY-2 cells within custom hydrogel scaffolds, this research demonstrates the creation of genetically modified engineered plant living materials with specific geometries and functionalities. Innovative granular hydrogel microparticles facilitate cell growth and DNA transformation, leading to those materials with unique pigmentation and fluorescent patterns. 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Advancing Engineered Plant Living Materials through Tobacco BY-2 Cell Growth and Transfection within Tailored Granular Hydrogel Scaffolds
In this study, an innovative approach is presented in the field of engineered plant living materials (EPLMs), leveraging a sophisticated interplay between synthetic biology and engineering. We detail a 3D bioprinting technique for the precise spatial patterning and genetic transformation of the tobacco BY-2 cell line within custom-engineered granular hydrogel scaffolds. Our methodology involves the integration of biocompatible hydrogel microparticles (HMPs) primed for 3D bioprinting with Agrobacterium tumefaciens capable of plant cell transfection, serving as the backbone for the simultaneous growth and transformation of tobacco BY-2 cells. This system facilitates the concurrent growth and genetic modification of tobacco BY-2 cells within our specially designed scaffolds. These scaffolds enable the cells to develop into predefined patterns while remaining conducive to the uptake of exogenous DNA. We showcase the versatility of this technology by fabricating EPLMs with unique structural and functional properties, exemplified by EPLMs exhibiting distinct pigmentation patterns. These patterns are achieved through the integration of the betalain biosynthetic pathway into tobacco BY-2 cells. Overall, our study represents a groundbreaking shift in the convergence of materials science and plant synthetic biology, offering promising avenues for the evolution of sustainable, adaptive, and responsive living material systems.
Utilizing Nicotiana tabacum BY-2 cells within custom hydrogel scaffolds, this research demonstrates the creation of genetically modified engineered plant living materials with specific geometries and functionalities. Innovative granular hydrogel microparticles facilitate cell growth and DNA transformation, leading to those materials with unique pigmentation and fluorescent patterns. This advancement opens new avenues in bioengineering, offering diverse applications in bioengineering and material science.
期刊介绍:
ACS Central Science publishes significant primary reports on research in chemistry and allied fields where chemical approaches are pivotal. As the first fully open-access journal by the American Chemical Society, it covers compelling and important contributions to the broad chemistry and scientific community. "Central science," a term popularized nearly 40 years ago, emphasizes chemistry's central role in connecting physical and life sciences, and fundamental sciences with applied disciplines like medicine and engineering. The journal focuses on exceptional quality articles, addressing advances in fundamental chemistry and interdisciplinary research.