在养殖的梭子蟹中发现病毒:一种再病毒和一种新的布尼亚病毒的基因组、系统发生学、组织病理学和显微镜鉴定。

IF 3.6 3区 生物学 Q1 ZOOLOGY Journal of invertebrate pathology Pub Date : 2024-04-27 DOI:10.1016/j.jip.2024.108118
Camila Prestes dos Santos Tavares , Samuel Paulo Cibulski , Gisela Geraldine Castilho-Westphal , Mingli Zhao , Ubiratan de Assis Teixeira Silva , Eric J. Schott , Antonio Ostrensky
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引用次数: 0

摘要

梭子蟹分布于世界各地,在水产养殖业中具有很高的价值。病毒感染是这些动物生存的主要限制因素,因此也是商业规模养殖成功的主要限制因素。然而,人们对全球养殖的梭子蟹所感染的病毒仍然缺乏了解。本文描述了Callinectes sapidus reovirus 2(CsRV2)的基因组序列和系统发育,并报告了在巴西南部养殖的Callinectes danae中发现的一种新的布尼亚病毒。CsRV2 基因组序列由 12 个 dsRNA 片段(20,909 nt)组成,编码 13 个蛋白质。预测的 RNA 依赖性 RNA 聚合酶(RdRp)与 Eriocheir sinensis reovirus 905 高度相似,表明 CsRV2 属于 Cardoreovirus 属。CsRV2 颗粒呈二十面体,直径约 65 nm,表现出典型的非卷曲再病毒形态。高通量测序数据揭示了另一种与布尼亚病毒相似的假定病毒基因组,名为Callinectes danae Portunibunyavirus 1(CdPBV1)。CdPBV1 基因组由 6,654 nt、3,120 nt 和 1,656 nt 单链 RNA 片段组成,每个片段编码一种蛋白质。每个片段都与欧洲滨蟹病毒 1 有很高的同一性,这表明 CdPBV1 是 Cruliviridae 科的一个新代表。CdPBV1 的假定球形颗粒直径为 120 nm,呈现典型的布尼亚病毒形态。组织病理学分析结果表明,这些新病毒会影响丹顶鹤的健康,进而影响其在人工饲养条件下的生存。因此,本文的研究结果应用于改善预防和病原体控制措施,并有助于巴西软壳蟹商业规模生产的发展和优化。
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Virus discovery in cultured portunid crabs: Genomic, phylogenetic, histopathological and microscopic characterization of a reovirus and a new bunyavirus

Portunid crabs are distributed worldwide and highly valued in aquaculture. Viral infections are the main limiting factor for the survival of these animals and, consequently, for the success of commercial-scale cultivation. However, there is still a lack of knowledge about the viruses that infect cultured portunid crabs worldwide. Herein, the genome sequence and phylogeny of Callinectes sapidus reovirus 2 (CsRV2) are described, and the discovery of a new bunyavirus in Callinectes danae cultured in southern Brazil is reported. The CsRV2 genome sequence consists of 12 dsRNA segments (20,909 nt) encode 13 proteins. The predicted RNA-dependent RNA polymerase (RdRp) shows a high level of similarity with that of Eriocheir sinensis reovirus 905, suggesting that CsRV2 belongs to the genus Cardoreovirus. The CsRV2 particles are icosahedral, measuring approximately 65 nm in diameter, and exhibit typical non-turreted reovirus morphology. High throughput sequencing data revealed the presence of an additional putative virus genome similar to bunyavirus, called Callinectes danae Portunibunyavirus 1 (CdPBV1). The CdPBV1 genome is tripartite, consisting of 6,654 nt, 3,120 nt and 1,656 nt single-stranded RNA segments that each encode a single protein. Each segment has a high identity with European shore crab virus 1, suggesting that CdPBV1 is a new representative of the family Cruliviridae. The putative spherical particles of CdPBV1 measure ∼120 nm in diameter and present a typical bunyavirus morphology. The results of the histopathological analysis suggest that these new viruses can affect the health and, consequently, the survival of C. danae in captivity. Therefore, the findings reported here should be used to improve prophylactic and pathogen control practices and contribute to the development and optimization of the production of soft-shell crabs on a commercial scale in Brazil.

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来源期刊
CiteScore
6.10
自引率
5.90%
发文量
94
审稿时长
1 months
期刊介绍: The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.
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