Lanfei Zhao, Amy Bernardo, Fanmei Kong, Wei Zhao, Yanhong Dong, Hyeonju Lee, Harold N Trick, Jessica Rupp Noller, Guihua Bai
{"title":"来自 Thinopyrum ponticum 的谷胱甘肽 S 转移酶能使 Fhb7 对小麦头孢镰刀菌枯萎病产生抗性。","authors":"Lanfei Zhao, Amy Bernardo, Fanmei Kong, Wei Zhao, Yanhong Dong, Hyeonju Lee, Harold N Trick, Jessica Rupp Noller, Guihua Bai","doi":"10.1094/PHYTO-03-24-0106-SC","DOIUrl":null,"url":null,"abstract":"<p><p>Fusarium head blight (FHB), mainly incited by <i>Fusarium graminearum</i>, has caused great losses in grain yield and quality of wheat globally. <i>Fhb7</i>, a major gene from 7E chromosome of <i>Thinopyrum ponticum</i>, confers broad resistance to multiple <i>Fusarium</i> species in wheat and has recently been cloned and identified as encoding a glutathione S-transferase (<i>GST</i>). However, some recent reports raised doubt about whether <i>GST</i> is the causal gene of <i>Fhb7</i>. To resolve the discrepancy and validate the gene function of <i>GST</i> in wheat, we phenotyped <i>Fhb7</i> near-isogenic lines (Jimai22-<i>Fhb7</i> versus Jimai22) and <i>GST</i> overexpressed lines for FHB resistance. Jimai22-<i>Fhb7</i> showed significantly higher FHB resistance with a lower percentage of symptomatic spikelets, <i>Fusarium</i>-damaged kernels, and deoxynivalenol content than susceptible Jimai22 in three experiments. All the positive <i>GST</i> transgenic lines driven by either the maize ubiquitin promoter or its native promoter with high gene expression in the wheat cultivar 'Fielder' showed high FHB resistance. Only one maize ubiquitin promoter-driven transgenic line showed low <i>GST</i> expression and similar susceptibility to Fielder, suggesting that high <i>GST</i> expression confers <i>Fhb7</i> resistance to FHB. Knockout of <i>GST</i> in the Jimai22-<i>Fhb7</i> line using CRISPR-Cas9-based gene editing showed significantly higher FHB susceptibility compared with the nonedited control plants. Therefore, we confirmed <i>GST</i> as the causal gene of <i>Fhb7</i> for FHB resistance. Considering its major effect on FHB resistance, pyramiding <i>Fhb7</i> with other quantitative trait loci has a great potential to create highly FHB-resistant wheat cultivars.</p>","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Glutathione S-Transferase from <i>Thinopyrum ponticum</i> Confers <i>Fhb7</i> Resistance to Fusarium Head Blight in Wheat.\",\"authors\":\"Lanfei Zhao, Amy Bernardo, Fanmei Kong, Wei Zhao, Yanhong Dong, Hyeonju Lee, Harold N Trick, Jessica Rupp Noller, Guihua Bai\",\"doi\":\"10.1094/PHYTO-03-24-0106-SC\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Fusarium head blight (FHB), mainly incited by <i>Fusarium graminearum</i>, has caused great losses in grain yield and quality of wheat globally. <i>Fhb7</i>, a major gene from 7E chromosome of <i>Thinopyrum ponticum</i>, confers broad resistance to multiple <i>Fusarium</i> species in wheat and has recently been cloned and identified as encoding a glutathione S-transferase (<i>GST</i>). However, some recent reports raised doubt about whether <i>GST</i> is the causal gene of <i>Fhb7</i>. To resolve the discrepancy and validate the gene function of <i>GST</i> in wheat, we phenotyped <i>Fhb7</i> near-isogenic lines (Jimai22-<i>Fhb7</i> versus Jimai22) and <i>GST</i> overexpressed lines for FHB resistance. Jimai22-<i>Fhb7</i> showed significantly higher FHB resistance with a lower percentage of symptomatic spikelets, <i>Fusarium</i>-damaged kernels, and deoxynivalenol content than susceptible Jimai22 in three experiments. All the positive <i>GST</i> transgenic lines driven by either the maize ubiquitin promoter or its native promoter with high gene expression in the wheat cultivar 'Fielder' showed high FHB resistance. Only one maize ubiquitin promoter-driven transgenic line showed low <i>GST</i> expression and similar susceptibility to Fielder, suggesting that high <i>GST</i> expression confers <i>Fhb7</i> resistance to FHB. Knockout of <i>GST</i> in the Jimai22-<i>Fhb7</i> line using CRISPR-Cas9-based gene editing showed significantly higher FHB susceptibility compared with the nonedited control plants. Therefore, we confirmed <i>GST</i> as the causal gene of <i>Fhb7</i> for FHB resistance. Considering its major effect on FHB resistance, pyramiding <i>Fhb7</i> with other quantitative trait loci has a great potential to create highly FHB-resistant wheat cultivars.</p>\",\"PeriodicalId\":20410,\"journal\":{\"name\":\"Phytopathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1094/PHYTO-03-24-0106-SC\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/7/11 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-03-24-0106-SC","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/11 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
A Glutathione S-Transferase from Thinopyrum ponticum Confers Fhb7 Resistance to Fusarium Head Blight in Wheat.
Fusarium head blight (FHB), mainly incited by Fusarium graminearum, has caused great losses in grain yield and quality of wheat globally. Fhb7, a major gene from 7E chromosome of Thinopyrum ponticum, confers broad resistance to multiple Fusarium species in wheat and has recently been cloned and identified as encoding a glutathione S-transferase (GST). However, some recent reports raised doubt about whether GST is the causal gene of Fhb7. To resolve the discrepancy and validate the gene function of GST in wheat, we phenotyped Fhb7 near-isogenic lines (Jimai22-Fhb7 versus Jimai22) and GST overexpressed lines for FHB resistance. Jimai22-Fhb7 showed significantly higher FHB resistance with a lower percentage of symptomatic spikelets, Fusarium-damaged kernels, and deoxynivalenol content than susceptible Jimai22 in three experiments. All the positive GST transgenic lines driven by either the maize ubiquitin promoter or its native promoter with high gene expression in the wheat cultivar 'Fielder' showed high FHB resistance. Only one maize ubiquitin promoter-driven transgenic line showed low GST expression and similar susceptibility to Fielder, suggesting that high GST expression confers Fhb7 resistance to FHB. Knockout of GST in the Jimai22-Fhb7 line using CRISPR-Cas9-based gene editing showed significantly higher FHB susceptibility compared with the nonedited control plants. Therefore, we confirmed GST as the causal gene of Fhb7 for FHB resistance. Considering its major effect on FHB resistance, pyramiding Fhb7 with other quantitative trait loci has a great potential to create highly FHB-resistant wheat cultivars.
期刊介绍:
Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.