David Mahouton Zoungbédji, Germain Gil Padonou, Arthur Sovi, Alphonse Keller Konkon, Albert Sourou Salako, Roseric Azondékon, Aboubakar Sidick, Juvénal Minassou Ahouandjinou, Linda Towakinou, Razaki Ossè, Rock Aïkpon, Cyriaque Affoukou, Lamine Baba-Moussa, Martin Akogbéto
{"title":"2023 年 6 月在西非贝宁开展撒网行动之前，Olyset® Plus、PermaNet® 3.0 和 Interceptor® G2 对抗除虫菊酯的冈比亚按蚊种群的生物功效。","authors":"David Mahouton Zoungbédji, Germain Gil Padonou, Arthur Sovi, Alphonse Keller Konkon, Albert Sourou Salako, Roseric Azondékon, Aboubakar Sidick, Juvénal Minassou Ahouandjinou, Linda Towakinou, Razaki Ossè, Rock Aïkpon, Cyriaque Affoukou, Lamine Baba-Moussa, Martin Akogbéto","doi":"10.1186/s41182-024-00599-z","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>This study investigates the effectiveness of new-generation mosquito nets, like Olyset<sup>®</sup> Plus and PermaNet<sup>®</sup> 3.0, and dual-action nets such as Interceptor<sup>®</sup> G2, against pyrethroid-resistant Anopheles gambiae mosquitoes following the 2023 mass distribution of long-lasting insecticidal nets in Benin.</p><p><strong>Methods: </strong>We tested wild mosquito populations from six communes in Benin against various pyrethroid (permethrin 0.75%, alphacypermethrin 0.05%, and deltamethrin 0.05%) using WHO tube tests. Additionally, we exposed mosquitoes to chlorfenapyr 100 µg/ml using the CDC bottle bioassay method. A subset of mosquitoes underwent biochemical and PCR tests to check the overexpression of metabolic enzymes and the Kdr L1014F mutation. We evaluated the effectiveness of Olyset<sup>®</sup> Plus, PermaNet<sup>®</sup> 3.0, and Interceptor<sup>®</sup> G2 nets using cone and tunnel tests on both laboratory and field populations of An. gambiae.</p><p><strong>Results: </strong>Overall, the highest mortality rate was 60% with pyrethroid and 98 to100% with chlorfenapyr. In cone tests, all three types of nets induced mortality rates above 80% in the susceptible laboratory strain of An. gambiae. Notably, Olyset<sup>®</sup> Plus showed the highest mortality rates for pyrethroid-resistant mosquitoes in cone tests, ranging from 81.03% (95% CI: 68.59-90.13) in Djougou to 96.08% (95% CI: 86.54-99.52) in Akpro-Missérété. PermaNet<sup>®</sup> 3.0 had variable rates, from 42.5% (95% CI: 27.04-59.11) in Djougou to 58.54% (95% CI: 42.11-73.68) in Porto-Novo. However, revealed good results for Interceptor<sup>®</sup> G2, with 94% (95% CI: 87.40-97.77) mortality and 89.09% blood sampling inhibition in local populations of An. gambiae. In comparison, Interceptor<sup>®</sup> had lower rates of 17% (95% CI: 10.23-25.82) and 60%, respectively.</p><p><strong>Conclusion: </strong>These results suggest that tunnel tests are effective for evaluating dual-active ingredient nets. Additionally, Interceptor<sup>®</sup> G2 and PBO nets like Olyset<sup>®</sup> Plus could be considered as alternatives against pyrethroid-resistant mosquitoes.</p>","PeriodicalId":23311,"journal":{"name":"Tropical Medicine and Health","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11059851/pdf/","citationCount":"0","resultStr":"{\"title\":\"Bio-efficacy of Olyset<sup>®</sup> Plus, PermaNet<sup>®</sup> 3.0 and Interceptor<sup>®</sup> G2 on pyrethroid-resistant populations of Anopheles gambiae s.l. prior to the June 2023 net distribution campaign in Benin, West Africa.\",\"authors\":\"David Mahouton Zoungbédji, Germain Gil Padonou, Arthur Sovi, Alphonse Keller Konkon, Albert Sourou Salako, Roseric Azondékon, Aboubakar Sidick, Juvénal Minassou Ahouandjinou, Linda Towakinou, Razaki Ossè, Rock Aïkpon, Cyriaque Affoukou, Lamine Baba-Moussa, Martin Akogbéto\",\"doi\":\"10.1186/s41182-024-00599-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>This study investigates the effectiveness of new-generation mosquito nets, like Olyset<sup>®</sup> Plus and PermaNet<sup>®</sup> 3.0, and dual-action nets such as Interceptor<sup>®</sup> G2, against pyrethroid-resistant Anopheles gambiae mosquitoes following the 2023 mass distribution of long-lasting insecticidal nets in Benin.</p><p><strong>Methods: </strong>We tested wild mosquito populations from six communes in Benin against various pyrethroid (permethrin 0.75%, alphacypermethrin 0.05%, and deltamethrin 0.05%) using WHO tube tests. Additionally, we exposed mosquitoes to chlorfenapyr 100 µg/ml using the CDC bottle bioassay method. A subset of mosquitoes underwent biochemical and PCR tests to check the overexpression of metabolic enzymes and the Kdr L1014F mutation. We evaluated the effectiveness of Olyset<sup>®</sup> Plus, PermaNet<sup>®</sup> 3.0, and Interceptor<sup>®</sup> G2 nets using cone and tunnel tests on both laboratory and field populations of An. gambiae.</p><p><strong>Results: </strong>Overall, the highest mortality rate was 60% with pyrethroid and 98 to100% with chlorfenapyr. In cone tests, all three types of nets induced mortality rates above 80% in the susceptible laboratory strain of An. gambiae. Notably, Olyset<sup>®</sup> Plus showed the highest mortality rates for pyrethroid-resistant mosquitoes in cone tests, ranging from 81.03% (95% CI: 68.59-90.13) in Djougou to 96.08% (95% CI: 86.54-99.52) in Akpro-Missérété. PermaNet<sup>®</sup> 3.0 had variable rates, from 42.5% (95% CI: 27.04-59.11) in Djougou to 58.54% (95% CI: 42.11-73.68) in Porto-Novo. However, revealed good results for Interceptor<sup>®</sup> G2, with 94% (95% CI: 87.40-97.77) mortality and 89.09% blood sampling inhibition in local populations of An. gambiae. In comparison, Interceptor<sup>®</sup> had lower rates of 17% (95% CI: 10.23-25.82) and 60%, respectively.</p><p><strong>Conclusion: </strong>These results suggest that tunnel tests are effective for evaluating dual-active ingredient nets. Additionally, Interceptor<sup>®</sup> G2 and PBO nets like Olyset<sup>®</sup> Plus could be considered as alternatives against pyrethroid-resistant mosquitoes.</p>\",\"PeriodicalId\":23311,\"journal\":{\"name\":\"Tropical Medicine and Health\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11059851/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical Medicine and Health\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s41182-024-00599-z\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"TROPICAL MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Medicine and Health","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s41182-024-00599-z","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"TROPICAL MEDICINE","Score":null,"Total":0}
Bio-efficacy of Olyset® Plus, PermaNet® 3.0 and Interceptor® G2 on pyrethroid-resistant populations of Anopheles gambiae s.l. prior to the June 2023 net distribution campaign in Benin, West Africa.
Background: This study investigates the effectiveness of new-generation mosquito nets, like Olyset® Plus and PermaNet® 3.0, and dual-action nets such as Interceptor® G2, against pyrethroid-resistant Anopheles gambiae mosquitoes following the 2023 mass distribution of long-lasting insecticidal nets in Benin.
Methods: We tested wild mosquito populations from six communes in Benin against various pyrethroid (permethrin 0.75%, alphacypermethrin 0.05%, and deltamethrin 0.05%) using WHO tube tests. Additionally, we exposed mosquitoes to chlorfenapyr 100 µg/ml using the CDC bottle bioassay method. A subset of mosquitoes underwent biochemical and PCR tests to check the overexpression of metabolic enzymes and the Kdr L1014F mutation. We evaluated the effectiveness of Olyset® Plus, PermaNet® 3.0, and Interceptor® G2 nets using cone and tunnel tests on both laboratory and field populations of An. gambiae.
Results: Overall, the highest mortality rate was 60% with pyrethroid and 98 to100% with chlorfenapyr. In cone tests, all three types of nets induced mortality rates above 80% in the susceptible laboratory strain of An. gambiae. Notably, Olyset® Plus showed the highest mortality rates for pyrethroid-resistant mosquitoes in cone tests, ranging from 81.03% (95% CI: 68.59-90.13) in Djougou to 96.08% (95% CI: 86.54-99.52) in Akpro-Missérété. PermaNet® 3.0 had variable rates, from 42.5% (95% CI: 27.04-59.11) in Djougou to 58.54% (95% CI: 42.11-73.68) in Porto-Novo. However, revealed good results for Interceptor® G2, with 94% (95% CI: 87.40-97.77) mortality and 89.09% blood sampling inhibition in local populations of An. gambiae. In comparison, Interceptor® had lower rates of 17% (95% CI: 10.23-25.82) and 60%, respectively.
Conclusion: These results suggest that tunnel tests are effective for evaluating dual-active ingredient nets. Additionally, Interceptor® G2 and PBO nets like Olyset® Plus could be considered as alternatives against pyrethroid-resistant mosquitoes.
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