纳米氧化铈颗粒对多柔比星诱导的心肌病代谢、凋亡、自噬和抗氧化变化的影响:可能的内在机制

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-10-01 DOI:10.61186/rbmb.12.3.495
Shorouk Elmorshdy Elsaeed Mohammed Elmorshdy, Gehan Ahmed Shaker, Zienab Helmy Eldken, Mahmoud Abdelbadie Salem, Amira Awadalla, Hany Mahmoud Abdel Shakour, Mohammed Elmahdy El Hosiny Sarhan, Abdelaziz Mohamed Hussein
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引用次数: 0

摘要

背景:方法:将 32 只成年雄性大鼠分为 4 组:i) 对照组;ii) NC 组;大鼠接受 NC(0.2 毫克/千克,i.p. 每天);iii) DOX 组;大鼠接受 DOX 4 毫克/千克(2 次注射,每次间隔 14 天);iv) DOX+NC 组,大鼠接受 DOX 但接受 NC、每天);iii)DOX 组;大鼠接受 DOX 4 毫克/千克(2 次注射,间隔 14 天);iv)DOX+NC 组,与 DOX 组相同,但大鼠接受 NC。实验结束时,记录心电图和心动图,评估心肌酶(CK-MB、LDH)和心肌氧化应激(MDA、过氧化氢酶和 GSH)的水平,以及 LC3 和 beclin1(自噬标记物)的表达、免疫组化法检测心脏组织中caspase3(细胞凋亡标志物)的表达,PCR法检测乙酰-CoA羧化酶α(ACCA)的表达,以及5'腺苷酸单磷酸激活蛋白激酶(AMPK)的水平。结果显示DOX组的校正QT间期延长,心肌酶(CK-MB和LDH)增加,心肌氧化应激(MDA高,过氧化氢酶和GSH低),心肌组织中ACCA、caspase-3、beclin1和LC3的表达,心肌AMPK水平降低,心肌收缩力(射血分数低,分数缩短)降低。另一方面,在服用 DOX 的同时服用 NC 可显著改善所有研究参数:结论:NC 对 DOX 诱导的心肌病有保护作用。结论:NC 对 DOX 诱导的心肌病具有心脏保护作用,这种作用可能是由于其抗氧化和抗凋亡作用,以及对 DOX 在心脏组织中诱导的自噬和代谢功能障碍的调节作用。
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Impact of Cerium Oxide Nanoparticles on Metabolic, Apoptotic, Autophagic and Antioxidant Changes in Doxorubicin-Induced Cardiomyopathy: Possible Underlying Mechanisms.

Background: In the current study, the effects of cerium oxide nanoparticles (nanocerium; NC) on doxorubicin (DOX)-induced cardiomyopathy and its possible underlying mechanisms were addressed.

Methods: 32 adult male rats were allocated into 4 groups; i) control group, ii) NC group; rats received NC (0.2 mg/kg, i.p., daily), iii) DOX group; rats received DOX 4 mg/kg (2 injections with a 14-day interval), and iv) DOX+NC group as DOX but rats received NC. At the end of the experiment, ECG and ECHO recordings and assessments of the levels of cardiac enzymes (CK-MB, LDH), and myocardial oxidative stress (MDA, catalase, and GSH), the expression of LC3 and beclin1 (markers of autophagy), caspase3 (marker of apoptosis) by immunohistochemistry, the expression of acetyl-CoA carboxylase alpha (ACCA) by PCR, and 5'adenosine monophosphate-activated protein kinase (AMPK) levels in the heart tissues were performed.

Results: The DOX group displayed a prolonged corrected QT interval, an increase in cardiac enzymes (CK-MB and LDH), myocardial oxidative stress (high MDA with low catalase and GSH), expression of ACCA, caspase-3, beclin1, and LC3 in myocardial tissues, with reduction in myocardial AMPK levels, and myocardial contractility (low ejection fraction, and fractional shortening). On the other hand, administration of NC with DOX resulted in significant improvement of all studied parameters.

Conclusion: NC offers a cardioprotective effect against DOX-induced cardiomyopathy. This effect might be due to its antioxidant and antiapoptotic effects as well as to the modulation of autophagy and metabolic dysfunctions induced by DOX in the heart tissues.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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