光敏剂存在时细胞对电离辐射和光的反应

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2024-03-07 DOI:10.1134/S0006350923050044
S. V. Akulinichev, S. I. Glukhov, A. V. Efremenko, D. A. Kokontsev, E. A. Kuznetsova, V. V. Martynova, A. V. Feofanov, I. A. Yakovlev
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摘要

摘要 研究了在人类肺腺癌 A549 细胞模型上联合使用 X 射线照射和光动力疗法的情况。研究发现,两种辐射的同步照射比它们的顺序使用或每种辐射的单独使用能更有效地降低肿瘤细胞的存活率。研究人员测定了两种辐照不同组合的细胞中涉及细胞周期控制(p21 和 DINOL)、细胞凋亡(BAX 和 PUMA)和 DNA 修复(PARP1、OGG1、Rad51 和 Lig4)的一些基因的 RNA 水平和表达情况。在所有处理中,DINOL 基因长非编码 RNA 的表达量最高。检测到的 BAX 和 PUMA 基因表达的增加表明细胞凋亡的发生。我们获得的结果证实了辐射和光动力疗法同时使用时的协同作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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The Cellular Response to Exposure to Ionizing Radiation and Light in the Presence of a Photosensitizer

The combined use of X-ray irradiation and photodynamic therapy on a model of human lung adenocarcinoma A549 cells has been investigated. It was found that synchronous exposure to two types of radiation contributed to a stronger decrease in the survival rate of tumor cells than their sequential use or each type of radiation separately. The levels of RNA and expression of a number of genes involved in cell cycle control (p21 and DINOL), apoptosis (BAX and PUMA) and DNA repair (PARP1, OGG1, Rad51, and Lig4) were determined for various combinations of these two types of irradiations of cells. The highest level of expression under all treatments was observed for the long noncoding RNA of DINOL gene. The detected increase in the expression of the BAX and PUMA genes indicated the development of apoptosis. The results we obtained confirmed the synergism of radiation and photodynamic therapy upon their simultaneous use.

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来源期刊
Biophysics
Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍: Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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