{"title":"从蒙古乌兰巴托市附近牛群中分离出的产志贺毒素大肠杆菌的特征。","authors":"Erdenebat Bulgan , Zolzaya Byambajav , Narantuya Ayushjav , Yuji Hirai , Misaki Tanaka , Nyam-Osor Purevdorj , Sandagdorj Badrakh , Akio Suzuki , Yusuke Komatsu , Toyotaka Sato , Motohiro Horiuchi","doi":"10.1016/j.jfp.2024.100294","DOIUrl":null,"url":null,"abstract":"<div><p>Shiga toxin-producin<em>g Escherichia coli</em> (STEC) are associated with severe infections including hemorrhagic colitis and hemolytic uremic syndrome in humans. Ruminants are known as reservoirs of STEC; however, no data are available on STEC in ruminants in Mongolia, where more than 5 million cattle and 25 million sheep are raised. To disclose the existence and characteristics of STEC in Mongolia, in this study, we isolated and characterized STEC from cattle in Mongolia. We collected 350 rectal swabs of cattle from 30 farms near Ulaanbaatar city and isolated 45 STEC from 21 farms. Rectal swabs were precultured with modified <em>Escherichia coli</em> broth and then inoculated to Cefixime-Tellurite Sorbitol MacConkey agar plate and/or CHROMagar STEC agar plate for the isolation of STEC. The isolation ratios in each farm were from 0% to 40%. Multiplex PCR for the estimation of O- and H-serotypes identified 12 O-genotypes (Og-types) and 11 <em>H</em>-genotypes (Hg-types) from 45 isolates; however, Og-types of 19 isolates could not be determined. <em>Stx</em> gene subtyping by PCR identified 2 <em>stx<sub>1</sub></em> subtypes (<em>1a</em> and <em>1c</em>) and 4 <em>stx<sub>2</sub></em> subtypes (<em>2a</em>, <em>2c</em>, <em>2d, and 2g</em>). Forty-five isolates were divided into 21 different groups based on the Og- and Hg-types, <em>stx</em> gene subtypes and the existence of virulence factors, <em>ehxA</em>, <em>eae</em>, and <em>saa</em>, which includes several major serotypes associated with human illness such as O26:<em>H</em>11 and O157:H7. The most dominant isolate, OgUT:H19 [<em>stx<sub>1a</sub></em> (+), <em>stx<sub>2a</sub></em> (+), <em>ehxA</em> (+) and <em>saa</em> (+)], was isolated from eight farms. This is the first report on the characterization of STEC in cattle in Mongolia, and the results suggest the importance of further monitoring of STEC contamination in the food chains as well as STEC infection in humans.</p></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0362028X24000784/pdfft?md5=92f630b649770212825ebeb1df8696f9&pid=1-s2.0-S0362028X24000784-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Characterization of Shiga Toxin-producing Escherichia coli Isolated from Cattle Around Ulaanbaatar City, Mongolia\",\"authors\":\"Erdenebat Bulgan , Zolzaya Byambajav , Narantuya Ayushjav , Yuji Hirai , Misaki Tanaka , Nyam-Osor Purevdorj , Sandagdorj Badrakh , Akio Suzuki , Yusuke Komatsu , Toyotaka Sato , Motohiro Horiuchi\",\"doi\":\"10.1016/j.jfp.2024.100294\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Shiga toxin-producin<em>g Escherichia coli</em> (STEC) are associated with severe infections including hemorrhagic colitis and hemolytic uremic syndrome in humans. Ruminants are known as reservoirs of STEC; however, no data are available on STEC in ruminants in Mongolia, where more than 5 million cattle and 25 million sheep are raised. To disclose the existence and characteristics of STEC in Mongolia, in this study, we isolated and characterized STEC from cattle in Mongolia. We collected 350 rectal swabs of cattle from 30 farms near Ulaanbaatar city and isolated 45 STEC from 21 farms. Rectal swabs were precultured with modified <em>Escherichia coli</em> broth and then inoculated to Cefixime-Tellurite Sorbitol MacConkey agar plate and/or CHROMagar STEC agar plate for the isolation of STEC. The isolation ratios in each farm were from 0% to 40%. Multiplex PCR for the estimation of O- and H-serotypes identified 12 O-genotypes (Og-types) and 11 <em>H</em>-genotypes (Hg-types) from 45 isolates; however, Og-types of 19 isolates could not be determined. <em>Stx</em> gene subtyping by PCR identified 2 <em>stx<sub>1</sub></em> subtypes (<em>1a</em> and <em>1c</em>) and 4 <em>stx<sub>2</sub></em> subtypes (<em>2a</em>, <em>2c</em>, <em>2d, and 2g</em>). Forty-five isolates were divided into 21 different groups based on the Og- and Hg-types, <em>stx</em> gene subtypes and the existence of virulence factors, <em>ehxA</em>, <em>eae</em>, and <em>saa</em>, which includes several major serotypes associated with human illness such as O26:<em>H</em>11 and O157:H7. The most dominant isolate, OgUT:H19 [<em>stx<sub>1a</sub></em> (+), <em>stx<sub>2a</sub></em> (+), <em>ehxA</em> (+) and <em>saa</em> (+)], was isolated from eight farms. This is the first report on the characterization of STEC in cattle in Mongolia, and the results suggest the importance of further monitoring of STEC contamination in the food chains as well as STEC infection in humans.</p></div>\",\"PeriodicalId\":15903,\"journal\":{\"name\":\"Journal of food protection\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-05-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0362028X24000784/pdfft?md5=92f630b649770212825ebeb1df8696f9&pid=1-s2.0-S0362028X24000784-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of food protection\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0362028X24000784\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of food protection","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0362028X24000784","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Characterization of Shiga Toxin-producing Escherichia coli Isolated from Cattle Around Ulaanbaatar City, Mongolia
Shiga toxin-producing Escherichia coli (STEC) are associated with severe infections including hemorrhagic colitis and hemolytic uremic syndrome in humans. Ruminants are known as reservoirs of STEC; however, no data are available on STEC in ruminants in Mongolia, where more than 5 million cattle and 25 million sheep are raised. To disclose the existence and characteristics of STEC in Mongolia, in this study, we isolated and characterized STEC from cattle in Mongolia. We collected 350 rectal swabs of cattle from 30 farms near Ulaanbaatar city and isolated 45 STEC from 21 farms. Rectal swabs were precultured with modified Escherichia coli broth and then inoculated to Cefixime-Tellurite Sorbitol MacConkey agar plate and/or CHROMagar STEC agar plate for the isolation of STEC. The isolation ratios in each farm were from 0% to 40%. Multiplex PCR for the estimation of O- and H-serotypes identified 12 O-genotypes (Og-types) and 11 H-genotypes (Hg-types) from 45 isolates; however, Og-types of 19 isolates could not be determined. Stx gene subtyping by PCR identified 2 stx1 subtypes (1a and 1c) and 4 stx2 subtypes (2a, 2c, 2d, and 2g). Forty-five isolates were divided into 21 different groups based on the Og- and Hg-types, stx gene subtypes and the existence of virulence factors, ehxA, eae, and saa, which includes several major serotypes associated with human illness such as O26:H11 and O157:H7. The most dominant isolate, OgUT:H19 [stx1a (+), stx2a (+), ehxA (+) and saa (+)], was isolated from eight farms. This is the first report on the characterization of STEC in cattle in Mongolia, and the results suggest the importance of further monitoring of STEC contamination in the food chains as well as STEC infection in humans.
期刊介绍:
The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with:
Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain;
Microbiological food quality and traditional/novel methods to assay microbiological food quality;
Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation;
Food fermentations and food-related probiotics;
Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers;
Risk assessments for food-related hazards;
Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods;
Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.