新型易吸附洗脱剂有助于解决非洲猪瘟病毒的环境污染问题。

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY AMB Express Pub Date : 2024-05-10 DOI:10.1186/s13568-024-01697-1
Li Zhang, Pengfei Zhao, Yingjun Xia, Yanli Hu, Chaofei Wang, Rui Fang, Junlong Zhao
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引用次数: 0

摘要

非洲猪瘟病毒(ASFV)是一种高致病性、传播迅速的病毒,在环境中具有很强的生存能力,这表明环境检测对所有养猪业的预防和控制都非常重要。然而,环境拭子的检测结果并不总能准确反映病毒污染状况,导致 ASFV 环境污染持续存在。qPCR分析表明,在环境拭子样品中,ASFV洗脱液处理的病毒拷贝数是传统洗脱液(0.85%生理盐水)处理的100倍。此外,屠宰场环境采样检测也验证了 ASFV 洗脱剂的高灵敏度。在土壤样品中,ASFV洗脱液的提取效果与TIANamp土壤DNA试剂盒相同,而0.85%盐溶液则没有提取效果。同时,这种洗脱液还能保护 ASFV 免受降解,无需冷藏即可在常温下运输样本。在临床实践中,我们监测了一个大型养猪场的 ASFV 环境污染状况。结果表明,每次环境消毒后,ASFV 的载量都会下降。这项研究为监测环境中 ASFV 的潜在威胁提供了有效的解决方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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A novel easy-to-desorb eluant contributes to address environmental contamination of African swine fever virus.

African swine fever virus (ASFV) is a highly pathogenic and rapidly disseminated virus with strong viability in the environment, suggesting the importance of environmental detection for prevention and control in all the pig industry. However, the detection results of environmental swabs cannot always reflect the accurate status of viral pollution, leading to persistent ASFV environmental contamination. In this study, we developed an ASFV eluant with higher environmental ASFV detection efficiency relative to 0.85% saline solution, which obtains the patent certificate issued by the China Intellectual Property Office (patent number:202010976050.9). qPCR analysis showed that in the environmental swab samples, the number of viral copies was 100 times higher for the ASFV eluant treatment than the traditional eluant treatment (0.85% saline solution). And besides, the high sensitivity of the ASFV eluant had be verified in a slaughterhouse environmental sampling detection. In soil samples, the ASFV eluent showed the same extraction effect as the TIANamp Soil DNA Kit, in contrast to no extraction effect for 0.85% saline solution. Simultaneously, this eluent could protect ASFV from degradation and allow the transportation of samples at ambient temperature without refrigeration. In clinical practice, we monitored the environmental contamination condition of the ASFV in a large-scale pig farm. The results shown that the ASFV load decreased after every disinfection in environment. This study provides an effective solution for surveilling the potential threat of ASFV in environment.

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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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