Mengying Wang, Dongqi Jiang, Xinyao Lu, Hong Zong and Bin Zhuge*,
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引用次数: 0
摘要
NAD 是一种氧化还原辅酶,是能量代谢的中心。在代谢工程改造中,NAD(H)供应不足往往会限制目标产物的积累。本研究发现,在有氧发酵过程中,甘油假丝酵母能够大量供应 NAD(H),其供应量是酿酒酵母的 7.6 倍。在不含氨基氮源的培养基中进行的有氧发酵表明,甘油酸酵母菌的 NAD 合成并不依赖于培养基中的 NAD 前体。反义 RNA 的抑制作用和转录本水平的检测表明,NAD 的主要供应途径是新生物合成途径。研究还进一步证明,通过 C. glycerinogenes ΔGPD 有氧发酵(80 克/升乙醇),NAD(H)供应不受代谢流量变化的影响。总之,C. glycerinogenes 提供大量 NAD(H) 的能力为解决合成生物学中的 NAD(H) 供应问题提供了一种新方法。
Large Flux Supply of NAD(H) under Aerobic Conditions by Candida glycerinogenes
NAD is a redox coenzyme and is the center of energy metabolism. In metabolic engineering modifications, an insufficient NAD(H) supply often limits the accumulation of target products. In this study, Candida glycerinogenes was found to be able to supply NAD(H) in large fluxes, up to 7.6 times more than Saccharomyces cerevisiae in aerobic fermentation. Aerobic fermentation in a medium without amino nitrogen sources demonstrated that C. glycerinogenes NAD synthesis was not dependent on NAD precursors in the medium. Inhibition by antisense RNA and the detection of transcript levels indicated that the main NAD supply pathway is the de novo biosynthesis pathway. It was further demonstrated that NAD(H) supply was unaffected by changes in metabolic flow through C. glycerinogenes ΔGPD aerobic fermentation (80 g/L ethanol). In conclusion, the ability of C. glycerinogenes to supply NAD(H) in large fluxes provides a new approach to solving the NAD(H) supply problem in synthetic biology.
期刊介绍:
The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism.
Topics may include, but are not limited to:
Design and optimization of genetic systems
Genetic circuit design and their principles for their organization into programs
Computational methods to aid the design of genetic systems
Experimental methods to quantify genetic parts, circuits, and metabolic fluxes
Genetic parts libraries: their creation, analysis, and ontological representation
Protein engineering including computational design
Metabolic engineering and cellular manufacturing, including biomass conversion
Natural product access, engineering, and production
Creative and innovative applications of cellular programming
Medical applications, tissue engineering, and the programming of therapeutic cells
Minimal cell design and construction
Genomics and genome replacement strategies
Viral engineering
Automated and robotic assembly platforms for synthetic biology
DNA synthesis methodologies
Metagenomics and synthetic metagenomic analysis
Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction
Gene optimization
Methods for genome-scale measurements of transcription and metabolomics
Systems biology and methods to integrate multiple data sources
in vitro and cell-free synthetic biology and molecular programming
Nucleic acid engineering.